We examine the transformative potential of artificial intelligence (AI) in medical imaging diagnosis, focusing on improving diagnostic accuracy and efficiency through advanced algorithms. It addresses the significant challenges preventing immediate clinical adoption of AI, specifically from technical, ethical, and legal perspectives. The aim is to highlight the current state of AI in medical imaging and outline the necessary steps to ensure safe, effective, and ethically sound clinical implementation. We conduct a comprehensive discussion, with special emphasis on the technical requirements for robust AI models, the ethical frameworks needed for responsible deployment, and the legal implications, including data privacy and regulatory compliance. Explainable artificial intelligence (XAI) is examined as a means to increase transparency and build trust among healthcare professionals and patients. The analysis reveals key challenges to AI integration in clinical settings, including the need for extensive high-quality datasets, model reliability, advanced infrastructure, and compliance with regulatory standards. The lack of explainability in AI outputs remains a barrier, with XAI identified as crucial for meeting transparency standards and enhancing trust among end users. Overcoming these barriers requires a collaborative, multidisciplinary approach to integrate AI into clinical practice responsibly. Addressing technical, ethical, and legal issues will support a softer transition, fostering a more accurate, efficient, and patient-centered healthcare system where AI augments traditional medical practices.

Zebrafish (Danio rerio) eyes are widely used in modeling studies of human ophthalmic diseases, including glaucoma and myopia. These pathologies cause morphological variations in the anterior chamber elements, which can be quantitatively measured using morphometric parameters, such as the corneal curvature, central corneal thickness, and anterior chamber angle. In the present work, an automated method is presented for iris and corneal segmentation, as well as the determination of the above-mentioned morphometry from optical coherence tomography (OCT) scans of zebrafish. The proposed method consists of four stages; namely, preprocessing, segmentation, postprocessing, and extraction of morphometric parameters. The first stage is composed of a combination of wavelet and Fourier transforms as well as gamma correction for artifact removal/reduction. The segmentation step is achieved using the U-net convolutional neural network. The postprocessing stage is composed of multilevel thresholding and morphological operations. Finally, three algorithms are proposed for automated morphological extraction in the last step. The morphology obtained using our automated framework is compared against manual measurements to assess the effectiveness of the method. The obtained results show that our scheme allows reliable determination of the morphometric parameters, thereby allowing efficient assessment for massive studies on zebrafish anterior chamber morphology using OCT scans.