A 16-day rearing trial was performed to investigate the influence of two supplemental levels (5% and 10%) of six dietary fat sources (linseed oil, peanut oil, coconut oil, soybean oil, lard oil and fish oil) on the growth, development and nutrient composition of black solider fly larvae. Our results demonstrated that the pre-pupa rate of larvae was linearly influenced by dietary C18:0, C18:3n-3 and C18:2n-6 content (pre-pupa rate = 0.927 × C18:0 content + 0.301 × C18:3n-3 content-0.258 × C18:2n-6 content p < 0.001)), while final body weight was linearly influenced by that of C16:0 (final body weight = 0.758 × C16:0 content, p = 0.004). Larval nutrient composition was significantly affected by dietary fat sources and levels, with crude protein, fat and ash content of larvae varying between 52.0 and 57.5, 15.0 and 23.8, and 5.6 and 7.2% dry matter. A higher level of C12:0 (17.4–28.5%), C14:0 (3.9–8.0%) and C16:1n-9 (1.3–4.3%) was determined in larvae fed the diets containing little of them. In comparison, C16:0, C18:1n-9, C18:2n-6 and C18:3n-3 proportions in larvae were linearly related with those in diets, with the slope of the linear equations varying from 0.39 to 0.60. It can be concluded that sufficient C16:0, C18:0 and C18:3n-3 supply is beneficial for larvae growth. Larvae could produce and retain C12:0, C14:0, and C16:1n-9 in vivo, but C16:0, C18:1n-9, C18:2n-6 and C18:3n-3 could only be partly incorporated from diets and the process may be enhanced by a higher amount of dietary fat. Based on the above observation, an accurately calculated amount of black soldier fly larvae could be formulated into aquafeed as the main source of saturated fatty acids and partial source of mono-unsaturated and poly-unsaturated fatty acids to save fish oil.