Currently there is a growing concern about the presence of emerging contaminants (EC) in water bodies and their potential ecotoxicological effects. Pharmaceuticals, a type of EC, are widely distributed in the environment and their main entry is through wastewater from treatment plants, since these systems are not designed to remove EC. In this sense, the photo-Fenton process, an advanced oxidation process, has proven to be highly efficient in degrading new potentially harmful contaminants. However, this process is affected by multiple factors, which makes it necessary to implement a methodological strategy to optimize the photo-Fenton process. Within these, the Taguchi method provides robust and low-cost solutions with the least number of experiments, providing knowledge of the contribution of each of the factors studied to the response variable. In addition to the above, the Taguchi method can be coupled to a Grey relational analysis (Grey–Taguchi method), which will allow the optimization of more than one response variable at the same time. This paper discusses the parameters that affect the photo-Fenton process and the application of designs of experiments to optimize the process.

A Gram-negative bacterium designated as strain TQ07 was isolated from a sawdust sample collected from a sawmill in the community of ''El Salto'' in the state of Durango, Mexico. This strain was previously reported for its ability to degrade chloranilic acid, a derivative of both biotic and abiotic degradation of pentachlorophenol. Genome sequencing was carried out using the Illumina NextSeq 500 platform. The draft genome of strain TQ07 was 5016,215 bp in length, with 4,504 protein-coding sequences and an average G + C content of 60.3 mol%. A phylogenetic tree supports the assignment of strain TQ07 as a member of the genus Herbaspirillum. Comparison of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values revealed that strain TQ07 shared 98.02% and 84.3% identity, respectively, with the genome of H. lusitanum P6-12 (DSM-17,154^T^). These values indicate that strain TQ07 belongs to the same species as strain P6-12. Other bacterial strains were found to contain all or some orthologs of genes involved in chloranilic acid degradation. Additionally, strain TQ07 possesses genes encoding proteins for Cd²⁺, Cu²⁺, Cr⁶⁺, and As⁵⁺ tolerance. The draft genome sequence of H. lusitanum TQ07 has been deposited in the DDBJ/EMBL/GenBank databases under the accession number PRJNA484228. This genome sequence data provides insightful information for taxonomic characterization and further biotechnological exploitation of H. lusitanum TQ07 for the bioremediation of treated wood.