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Maria De Lourdes T. M. Polizeli
Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14050-901, Brazil

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Short Biography

Graduated in Pharmacy and Biochemistry (1982) from the current Faculty of Pharmaceutical Sciences of Ribeirão Preto, FCFRP, University of São Paulo, USP. Master's degree in Biological Sciences (Biochemistry) from Ribeirão Preto Medical School, USP (1986) and a Ph.D. in Biochemistry from the same institution (1991). Post-doctorate in Cell Biology (1992/1993). Full Professor at the Department of Biology at FFCLRP-USP from 2018. Worked on the Commission Research FFCLRP-USP as Vice-President (2016-2018), and President (2018-2019). Vice-President of the Internal Biosafety Commission of FFCLRP-USP (2017-2019) and was a member of the Human Research Ethics Committee with FCFRP (2004 to 2014). At graduation, she teaches the disciplines Cellular Biology and Bioprospecting for Biotechnological Processes and various disciplines of Internships and Course Conclusion. She teaches the disciplines Cellular Signaling and Enzyme Immobilization. Research interests are focused on the areas of Cell Biology, Microbiology, Bioprocesses and Fermentation, Biochemistry, and Enzymology. She mainly works on the following themes: filamentous fungi, lipases, amylases, xylanases, and other enzymatic systems that act in the degradation of plant cell walls. She also maintains international research interactions with several countries. Currently, Dr. Polizeli has 170 manuscripts published, 11 chapters, and 5 books.

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Journal article
Published: 31 August 2021 in International Journal of Molecular Sciences
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Since laccase acts specifically in lignin, the major contributor to biomass recalcitrance, this biocatalyst represents an important alternative to the pretreatment of lignocellulosic biomass. Therefore, this study investigates the laccase pretreatment and climate change effects on the hydrolytic performance of Panicum maximum. Through a Trop-T-FACE system, P. maximum grew under current (Control (C)) and future climate conditions: elevated temperature (2 °C more than the ambient canopy temperature) combined with elevated atmospheric CO2 concentration(600 μmol mol−1), name as eT+eC. Pretreatment using a laccase-rich crude extract from Lentinus sajor caju was optimized through statistical strategies, resulting in an increase in the sugar yield of P. maximum biomass (up to 57%) comparing to non-treated biomass and enabling hydrolysis at higher solid loading, achieving up to 26 g L−1. These increments are related to lignin removal (up to 46%) and lignin hydrophilization catalyzed by laccase. Results from SEM, CLSM, FTIR, and GC-MS supported the laccase-catalyzed lignin removal. Moreover, laccase mitigates climate effects, and no significant differences in hydrolytic potential were found between C and eT+eC groups. This study shows that crude laccase pretreatment is a potential and sustainable method for biorefinery solutions and helped establish P. maximum as a promising energy crop.

ACS Style

Emanuelle Neiverth de Freitas; Robson Carlos Alnoch; Alex Graça Contato; Karoline Maria V. Nogueira; Eduardo José Crevelin; Luiz Alberto Beraldo de Moraes; Roberto Nascimento Silva; Carlos Alberto Martínez; Maria De Lourdes T. M. Polizeli. Enzymatic Pretreatment with Laccases from Lentinus sajor-caju Induces Structural Modification in Lignin and Enhances the Digestibility of Tropical Forage Grass (Panicum maximum) Grown under Future Climate Conditions. International Journal of Molecular Sciences 2021, 22, 9445 .

AMA Style

Emanuelle Neiverth de Freitas, Robson Carlos Alnoch, Alex Graça Contato, Karoline Maria V. Nogueira, Eduardo José Crevelin, Luiz Alberto Beraldo de Moraes, Roberto Nascimento Silva, Carlos Alberto Martínez, Maria De Lourdes T. M. Polizeli. Enzymatic Pretreatment with Laccases from Lentinus sajor-caju Induces Structural Modification in Lignin and Enhances the Digestibility of Tropical Forage Grass (Panicum maximum) Grown under Future Climate Conditions. International Journal of Molecular Sciences. 2021; 22 (17):9445.

Chicago/Turabian Style

Emanuelle Neiverth de Freitas; Robson Carlos Alnoch; Alex Graça Contato; Karoline Maria V. Nogueira; Eduardo José Crevelin; Luiz Alberto Beraldo de Moraes; Roberto Nascimento Silva; Carlos Alberto Martínez; Maria De Lourdes T. M. Polizeli. 2021. "Enzymatic Pretreatment with Laccases from Lentinus sajor-caju Induces Structural Modification in Lignin and Enhances the Digestibility of Tropical Forage Grass (Panicum maximum) Grown under Future Climate Conditions." International Journal of Molecular Sciences 22, no. 17: 9445.

Journal article
Published: 26 August 2021 in Industrial Crops and Products
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Panicum maximum represents an alternative as a dedicated energy crop for biorefinery. However, both pretreatment step and climate conditions impact its chemical composition and possibly its hydrolytic performance. Therefore, this study looks into integrating the future effect of the climate conditions and the pretreatment methods on the hydrolytic performance of P. maximum. Through a Trop-T-FACE system, P. maximum grown under three hypothetical future climate conditions: elevated temperature ((eT), which is 2 °C more than the ambient canopy temperature), elevated atmospheric CO2 concentration ((eC) up to 600 μmol mol-1) and the combined effects (eT + eC). Subsequently, the groups were subjected to the liquid hot water (LHW) and organosolv (ORG) pretreatments, which enhanced biomass digestibility by mostly hemicellulose and lignin removal, respectively. Furthermore, optimized hydrolysis and fluorescent protein-tagged carbohydrate-binding modules binding suggested that P. maximum pretreated by LHW has greater saccharification yields and higher cellulose surface accessibility/exposure. This shows that hemicellulose directly impacts P. maximum recalcitrance, and its removal was the optimum pretreatment strategy. Concerning the studied expected future climate conditions, LHW samples did not show significant differences in hydrolysis yields at low solids loading (2%). However, at 10 % solids, eC had higher conversion yields (likely due to minimum end-product inhibition). Similarly, for organosolv pretreated samples, eC (at 2 and 10 % solid loading) exhibited greater cellulose-glucan conversion yields among the climate conditions groups and the highest cellulose accessibility. Thus, this work contributes to understand better the influence of both studied pretreatment methods and future climate conditions on the conversion efficiency of a critical energy crop and might assist in selecting the best pretreatment method to mitigate the climate effects and achieve greater hydrolysis yields.

ACS Style

Emanuelle N. de Freitas; Vinay Khatri; Jie Wu; Masatsugu Takada; Ana Silvia de A. Scarcella; Carlos Alberto Martinez; Jack N. Saddler; Maria De Lourdes T.M. Polizeli. Structural and compositional changes induced by hydrothermal and organosolv pretreatments impacts enzymatic hydrolysis of a tropical forage grass grown under future climate conditions. Industrial Crops and Products 2021, 171, 113937 .

AMA Style

Emanuelle N. de Freitas, Vinay Khatri, Jie Wu, Masatsugu Takada, Ana Silvia de A. Scarcella, Carlos Alberto Martinez, Jack N. Saddler, Maria De Lourdes T.M. Polizeli. Structural and compositional changes induced by hydrothermal and organosolv pretreatments impacts enzymatic hydrolysis of a tropical forage grass grown under future climate conditions. Industrial Crops and Products. 2021; 171 ():113937.

Chicago/Turabian Style

Emanuelle N. de Freitas; Vinay Khatri; Jie Wu; Masatsugu Takada; Ana Silvia de A. Scarcella; Carlos Alberto Martinez; Jack N. Saddler; Maria De Lourdes T.M. Polizeli. 2021. "Structural and compositional changes induced by hydrothermal and organosolv pretreatments impacts enzymatic hydrolysis of a tropical forage grass grown under future climate conditions." Industrial Crops and Products 171, no. : 113937.

Journal article
Published: 15 July 2021 in International Journal of Biological Macromolecules
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A purified exo-polygalacturonase of Neosartorya glabra (EplNg) was successfully characterized. EplNg native presented 68.2 kDa, with 32% carbohydrate content. The deglycosylated form showed 46.3 kDa and isoelectric point of 5.4. The identity of EplNg was confirmed as an exo-polygalacturonase class I (EC 3.2.1.67) using mass spectrometry and Western-Blotting. Capillary electrophoresis indicated that only galacturonic acid was released by the action of EplNg on sodium polypectate, confirming an exoenzyme character. The structural model confers that EplNg has a core formed by twisted parallel β-sheets structure. Among twelve putative cysteines, ten were predicted to form disulfide bridges. The catalytic triad predicted is composed of Asp223, Asp245, and Asp246 aligned along with a distance in 4–5 Å, suggesting that EplNg probably does not perform the standard inverting catalytic mechanism described for the GH28 family. EplNg was active from 30 to 90 °C, with maximum activity at 65 °C, pH 5.0. The Km and Vmax determined using sodium polypectate were 6.9 mg·mL−1 and Vmax 690 μmol·min−1.mg−1, respectively. EplNg was active and stable over a wide range of pH values and temperatures, confirming the interesting properties EplNg and provide a basis for the development of the enzyme in different biotechnological processes.

ACS Style

Carla Cristina Villela Desagiacomo; Robson Carlos Alnoch; Vanessa Elisa Pinheiro; Mariana Cereia; Carla Botelho Machado; André Damasio; Marlei Josiele Augusto; Wellington Pedersoli; Roberto Nascimento Silva; Maria De Lourdes Teixeira De Moraes Polizeli. Structural model and functional properties of an exo-polygalacturonase from Neosartorya glabra. International Journal of Biological Macromolecules 2021, 186, 909 -918.

AMA Style

Carla Cristina Villela Desagiacomo, Robson Carlos Alnoch, Vanessa Elisa Pinheiro, Mariana Cereia, Carla Botelho Machado, André Damasio, Marlei Josiele Augusto, Wellington Pedersoli, Roberto Nascimento Silva, Maria De Lourdes Teixeira De Moraes Polizeli. Structural model and functional properties of an exo-polygalacturonase from Neosartorya glabra. International Journal of Biological Macromolecules. 2021; 186 ():909-918.

Chicago/Turabian Style

Carla Cristina Villela Desagiacomo; Robson Carlos Alnoch; Vanessa Elisa Pinheiro; Mariana Cereia; Carla Botelho Machado; André Damasio; Marlei Josiele Augusto; Wellington Pedersoli; Roberto Nascimento Silva; Maria De Lourdes Teixeira De Moraes Polizeli. 2021. "Structural model and functional properties of an exo-polygalacturonase from Neosartorya glabra." International Journal of Biological Macromolecules 186, no. : 909-918.

Original article
Published: 02 June 2021 in Biofuels, Bioproducts and Biorefining
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Biofuels contribute environment-friendly, renewable energy, minimizing dependence on fossil energy. The pretreatment of biomass is a practical step that accelerates and facilitates the hydrolysis of complex materials. This work aimed to screen, select, and study cocktail formulations for biomass hydrolysis, where the enzymes were provided both from a commercial source (Trichoderma reesei, Novozymes®) and through the cultivation of Aspergillus brasiliensis and Aspergillus tamarii Kita. Experimental mixture designs were used to optimize the enzymatic conversion of substrates into simple sugars. A crude extract rich in amylase (AAB) had a significant favorable influence on cornmeal hydrolysis by maximizing the yield of reducing sugars (RS) (173 μmol mL–1). Celluclast™, rich in cellulase, significantly affected the hydrolysis of banana peel, maximizing the RS yield (175 μmol mL–1). Variable degrees of enzyme synergism were evident from statistical analysis of the biomass hydrolysis. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

ACS Style

Vanessa Elisa Pinheiro; Ilona Sárvári Horváth; Magnus Lundin; Maria De Lourdes Teixeira De Moraes Polizeli. Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy. Biofuels, Bioproducts and Biorefining 2021, 1 .

AMA Style

Vanessa Elisa Pinheiro, Ilona Sárvári Horváth, Magnus Lundin, Maria De Lourdes Teixeira De Moraes Polizeli. Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy. Biofuels, Bioproducts and Biorefining. 2021; ():1.

Chicago/Turabian Style

Vanessa Elisa Pinheiro; Ilona Sárvári Horváth; Magnus Lundin; Maria De Lourdes Teixeira De Moraes Polizeli. 2021. "Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy." Biofuels, Bioproducts and Biorefining , no. : 1.

Journal article
Published: 18 April 2021 in Biotechnology Reports
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β-Glucosidases are a limiting factor in the conversion of cellulose to glucose for the subsequent ethanol production. Here, β-glucosidase production by Malbranchea pulchella was optimized using Composite Central Designs and Response Surface Methodologies from a medium designed. The coefficient of determination (R2) was 0.9960, F-value was very high, and the lack of fit was found to be non-significant. This indicates a statistic valid and predictive result. M. pulchella enzymatic extract was successfully tested as an enzymatic cocktail in a mixture design using sugarcane bagasse, soybean hull and barley bagasse. We proved that the optimization of the β-glucosidase production and the application in hydrolysis using unexpansive biomass and agricultural wastes can be accomplished by means of statistical methodologies. The strategy presented here can be useful for the improvement of enzyme production and the hydrolysis process, arising as an alternative for bioeconomy.

ACS Style

Lummy Maria Oliveira Monteiro; Ana Claudia Vici; Josana Maria Messias; Paulo Ricardo Heinen; Vanessa Elisa Pinheiro; Carem Gledes Vargas Rechia; Marcos S. Buckeridge; Maria De Lourdes Teixeira De Moraes Polizeli. Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs. Biotechnology Reports 2021, 30, e00618 .

AMA Style

Lummy Maria Oliveira Monteiro, Ana Claudia Vici, Josana Maria Messias, Paulo Ricardo Heinen, Vanessa Elisa Pinheiro, Carem Gledes Vargas Rechia, Marcos S. Buckeridge, Maria De Lourdes Teixeira De Moraes Polizeli. Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs. Biotechnology Reports. 2021; 30 ():e00618.

Chicago/Turabian Style

Lummy Maria Oliveira Monteiro; Ana Claudia Vici; Josana Maria Messias; Paulo Ricardo Heinen; Vanessa Elisa Pinheiro; Carem Gledes Vargas Rechia; Marcos S. Buckeridge; Maria De Lourdes Teixeira De Moraes Polizeli. 2021. "Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs." Biotechnology Reports 30, no. : e00618.

Review article
Published: 15 March 2021 in Journal of Agricultural and Food Chemistry
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Interest in chitin-degrading enzymes has grown over the years, and microbial chitinases are the most attractive and promising candidates for the control of plant pests (fungi and insects). Currently, there are many studies on chitinases produced by cultivable microorganisms; however, almost none of them have achieved acceptable applicability as a biopesticide in the field. Approximately 99% of the microorganisms from soil cannot be isolated by conventional culture-dependent methods, thus having an enormous biotechnological/genetic potential to be explored. On the basis of this, the present paper aims to provide a brief overview of the metagenomic opportunities that have been emerging and allowing access to the biochemical potential of uncultivable microorganisms through the direct mining of DNA sequences recovered from the environment. This work also shortly discussed the future perspectives of functional and sequence-based metagenomic approaches for the identification of new chitinase-coding genes with potential for applications in several agricultural and biotechnological industries, especially in biological control.

ACS Style

Thiago Machado Pasin; Tássio Brito de Oliveira; Ana Sílvia De Almeida Scarcella; Maria De Lourdes Teixeira De Moraes Polizeli; María-Eugenia Guazzaroni. Perspectives on Expanding the Repertoire of Novel Microbial Chitinases for Biological Control. Journal of Agricultural and Food Chemistry 2021, 69, 3284 -3288.

AMA Style

Thiago Machado Pasin, Tássio Brito de Oliveira, Ana Sílvia De Almeida Scarcella, Maria De Lourdes Teixeira De Moraes Polizeli, María-Eugenia Guazzaroni. Perspectives on Expanding the Repertoire of Novel Microbial Chitinases for Biological Control. Journal of Agricultural and Food Chemistry. 2021; 69 (11):3284-3288.

Chicago/Turabian Style

Thiago Machado Pasin; Tássio Brito de Oliveira; Ana Sílvia De Almeida Scarcella; Maria De Lourdes Teixeira De Moraes Polizeli; María-Eugenia Guazzaroni. 2021. "Perspectives on Expanding the Repertoire of Novel Microbial Chitinases for Biological Control." Journal of Agricultural and Food Chemistry 69, no. 11: 3284-3288.

Journal article
Published: 13 March 2021 in Energy
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Lignocellulosic biomasses are promising sources for the obtaining of clean energy through their bioconversion into ethanol. Their saccharification involves a multi-enzymatic system. Here, we evaluated the hydrolysis of a mixture of sugarcane bagasse varieties (SCB), Energy cane fiber (EC), and sugarcane bagasse from the SP80-3280 variety, all in natura, using fungal enzymatic extracts obtained from Mycothermus thermophilus and Trichoderma reesei RP698 cultures supplemented with various agro-industrial residues. The enzymatic extracts from both fungi, when grown in a corn cob and corn straw, led to the highest sugarcane hydrolysis. For M. thermophilus, the reducing sugars released (mg/mL) were 1.21 ± 0.12, 1.25 ± 0.14, and 0.98 ± 0.05 for SCB, EC and SP80-3280, respectively; for T. reesei, the reducing sugars were 0.84 ± 0.08, 0.89 ± 0.06 and 0.68 ± 0.03 for SCB, SP80-3280, and EC, respectively. The cocktail obtained from the co-cultivation of these fungi in corn straw at 35 °C showed the best hydrolysis results, the release of sugars (mg/mL) was 1.31 ± 0.06 (SCB), 2.18 ± 0.08 (EC) and 1.67 ± 0.09 (SP80-3280). Scanning electron microscopy and thermogravimetric analysis confirmed changes in the fiber structures after enzymatic hydrolysis. Thus, these fungi were shown to be promising for an enzymatic cocktail design and sugarcane biomass saccharification.

ACS Style

Ana Sílvia De Almeida Scarcella; Thiago Machado Pasin; Tássio Brito de Oliveira; Rosymar Coutinho de Lucas; Monica Stropa Ferreira-Nozawa; Emanuelle Neiverth de Freitas; Ana Claudia Vici; Marcos Silveira Buckeridge; Michele Michelin; Maria De Lourdes Teixeira De Moraes Polizeli. Saccharification of different sugarcane bagasse varieties by enzymatic cocktails produced by Mycothermus thermophilus and Trichoderma reesei RP698 cultures in agro-industrial residues. Energy 2021, 226, 120360 .

AMA Style

Ana Sílvia De Almeida Scarcella, Thiago Machado Pasin, Tássio Brito de Oliveira, Rosymar Coutinho de Lucas, Monica Stropa Ferreira-Nozawa, Emanuelle Neiverth de Freitas, Ana Claudia Vici, Marcos Silveira Buckeridge, Michele Michelin, Maria De Lourdes Teixeira De Moraes Polizeli. Saccharification of different sugarcane bagasse varieties by enzymatic cocktails produced by Mycothermus thermophilus and Trichoderma reesei RP698 cultures in agro-industrial residues. Energy. 2021; 226 ():120360.

Chicago/Turabian Style

Ana Sílvia De Almeida Scarcella; Thiago Machado Pasin; Tássio Brito de Oliveira; Rosymar Coutinho de Lucas; Monica Stropa Ferreira-Nozawa; Emanuelle Neiverth de Freitas; Ana Claudia Vici; Marcos Silveira Buckeridge; Michele Michelin; Maria De Lourdes Teixeira De Moraes Polizeli. 2021. "Saccharification of different sugarcane bagasse varieties by enzymatic cocktails produced by Mycothermus thermophilus and Trichoderma reesei RP698 cultures in agro-industrial residues." Energy 226, no. : 120360.

Journal article
Published: 05 March 2021 in Microorganisms
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The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using Hymenaea courbaril and Tamarindus indica seeds as carbon sources. α-L-arabinofuranosidase, acetyl xylan esterase, endo-1,5-α-L-arabinanase, β-D-galactosidase, β-D-glucosidase, β-glucanase, β-D-xylosidase, cellobiohydrolase, endoglucanase, lichenase, mannanase, polygalacturonase, endo-1,4-β-xylanase, and xyloglucanase activities were determined. The enzymes were produced for eight filamentous fungi: Aspergillus fumigatus, Trametes hirsuta, Lasiodiplodia sp., two strains of Trichoderma longibrachiatum, Neocosmospora perseae, Fusarium sp. and Thermothelomyces thermophilus. The best producers concerning enzymatic activity were T. thermophilus and T. longibrachiatum. The optimal conditions for enzyme production were the media supplemented with tamarind seeds, under agitation, for 72 h. This analysis was essential to demonstrate that cultivation conditions, static and under agitation, exert strong influences on the production of several enzymes produced by different fungi. The kind of sugarcane, pretreatment used, microorganisms, and carbon sources proved limiting sugar profile factors.

ACS Style

Alex Contato; Tássio de Oliveira; Guilherme Aranha; Emanuelle de Freitas; Ana Vici; Karoline Nogueira; Rosymar de Lucas; Ana Scarcella; Marcos Buckeridge; Roberto Silva; Maria Polizeli. Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse. Microorganisms 2021, 9, 533 .

AMA Style

Alex Contato, Tássio de Oliveira, Guilherme Aranha, Emanuelle de Freitas, Ana Vici, Karoline Nogueira, Rosymar de Lucas, Ana Scarcella, Marcos Buckeridge, Roberto Silva, Maria Polizeli. Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse. Microorganisms. 2021; 9 (3):533.

Chicago/Turabian Style

Alex Contato; Tássio de Oliveira; Guilherme Aranha; Emanuelle de Freitas; Ana Vici; Karoline Nogueira; Rosymar de Lucas; Ana Scarcella; Marcos Buckeridge; Roberto Silva; Maria Polizeli. 2021. "Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse." Microorganisms 9, no. 3: 533.

Original article
Published: 28 January 2021 in Biomass Conversion and Biorefinery
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Economic interest in sugarcane bagasse has significantly increased in recent years due to the worldwide demand for sustainable energy production. The use of sugarcane bagasse for holocellulase production has been a strategy for bioconversion of lignocellulosic residues into second-generation ethanol. The fungi secrete to the culture medium a cocktail of enzymes necessary to convert biomass into nutrients. Thus, this study aimed to analyze the production profile of holocellulases from Aspergillus and Humicola species, Trichoderma reesei RP698, and Mycothermus thermophilus grown in sugarcane bagasse, culm of energy cane, and culm of sugarcane SP80-3280. The capacity of the enzymatic pools in the hydrolysis of cell walls of these sugarcane varieties was also verified. M. thermophilus was the best producer of endoglucanase, cellobiohydrolase, β-glucosidase, xylanase, β-xylosidase, xyloglucanase, arabinanase, arabinofuranosidase, mannanase, and acetyl xylan esterase. T. reesei RP698 also produced and secreted a wide range of holocellulases to the medium. The saccharification of sugarcane bagasse, energy cane, and sugarcane SP80-3280 by the enzymatic cocktails obtained from M. thermophilus released 0.87 ± 0.05 mg.mL−1, 0.88 ± 0.07 mg.mL−1, and 1.10 ± 0.08 mg.mL−1 of reducing sugars, respectively. However, the application of T. reesei RP698 extracts showed a release of 0.85 ± 0.03 mg.mL−1, 0.40 ± 0.03 mg.mL−1, and 0.83 ± 0.03 mg.mL−1 of reducing sugars. Therefore, T. reesei RP698 and M. thermophilus showed to be good holocellulase producers, and their crude extracts presented a great capacity for the hydrolysis of the different kinds of sugarcane residues.

ACS Style

Ana Sílvia De Almeida Scarcella; Thiago Machado Pasin; Rosymar Coutinho De Lucas; Monica Stropa Ferreira-Nozawa; Tássio Brito De Oliveira; Alex Graça Contato; Adriana Grandis; Marcos Silveira Buckeridge; Maria De Lourdes Teixeira De Moraes Polizeli. Holocellulase production by filamentous fungi: potential in the hydrolysis of energy cane and other sugarcane varieties. Biomass Conversion and Biorefinery 2021, 1 -12.

AMA Style

Ana Sílvia De Almeida Scarcella, Thiago Machado Pasin, Rosymar Coutinho De Lucas, Monica Stropa Ferreira-Nozawa, Tássio Brito De Oliveira, Alex Graça Contato, Adriana Grandis, Marcos Silveira Buckeridge, Maria De Lourdes Teixeira De Moraes Polizeli. Holocellulase production by filamentous fungi: potential in the hydrolysis of energy cane and other sugarcane varieties. Biomass Conversion and Biorefinery. 2021; ():1-12.

Chicago/Turabian Style

Ana Sílvia De Almeida Scarcella; Thiago Machado Pasin; Rosymar Coutinho De Lucas; Monica Stropa Ferreira-Nozawa; Tássio Brito De Oliveira; Alex Graça Contato; Adriana Grandis; Marcos Silveira Buckeridge; Maria De Lourdes Teixeira De Moraes Polizeli. 2021. "Holocellulase production by filamentous fungi: potential in the hydrolysis of energy cane and other sugarcane varieties." Biomass Conversion and Biorefinery , no. : 1-12.

Journal article
Published: 17 November 2020 in Renewable Energy
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The plant cell wall is the most abundant carbon reservoir in nature and is a renewable source of biofuels. To break down this biomass and convert it into fermentable sugars, a set of multiple enzymes is needed. Here, we characterize the enzymatic repertoire necessary for the degradation of sugarcane bagasse “in natura” and pre-treated using Aspergillus clavatus as a model. 135 unique peptides were identified by Mass Spectrometry MS/MS. 23 of these proteins belong to classes of enzymes involved in biomass degradation and were differentially expressed on various substrates. Each pretreatment changed the sugarcane bagasse composition, which, in turn, led to the differential expression of A. clavatus holocellulases. The deconstruction of “in natura” bagasse demanded the largest set of enzymes due to the structural complexity of this material. Not only different sources of biomass but also different pretreatments of the same source will determine the enzymes required for the most efficient biomass conversion, avoiding the use of non-essential enzymes and consequent financial expense. Understanding A. clavatus nutritional strategies by proteomic analysis of secretome can improve the technology applied to biomass conversion and by-product synthesis.

ACS Style

Rosymar Coutinho de Lucas; Tássio Brito de Oliveira; Matheus Sanitá Lima; Thiago Machado Pasin; Ana Sílvia De Almeida Scarcella; Liliane Fraga Costa Ribeiro; Caio Carvalho; André Ricardo De Lima Damasio; Marcos Silveira Buckeridge; Rolf Alexander Prade; Fernando Segato; Maria De Lourdes Teixeira De Moraes Polizeli. The profile secretion of Aspergillus clavatus: Different pre-treatments of sugarcane bagasse distinctly induces holocellulases for the lignocellulosic biomass conversion into sugar. Renewable Energy 2020, 165, 748 -757.

AMA Style

Rosymar Coutinho de Lucas, Tássio Brito de Oliveira, Matheus Sanitá Lima, Thiago Machado Pasin, Ana Sílvia De Almeida Scarcella, Liliane Fraga Costa Ribeiro, Caio Carvalho, André Ricardo De Lima Damasio, Marcos Silveira Buckeridge, Rolf Alexander Prade, Fernando Segato, Maria De Lourdes Teixeira De Moraes Polizeli. The profile secretion of Aspergillus clavatus: Different pre-treatments of sugarcane bagasse distinctly induces holocellulases for the lignocellulosic biomass conversion into sugar. Renewable Energy. 2020; 165 ():748-757.

Chicago/Turabian Style

Rosymar Coutinho de Lucas; Tássio Brito de Oliveira; Matheus Sanitá Lima; Thiago Machado Pasin; Ana Sílvia De Almeida Scarcella; Liliane Fraga Costa Ribeiro; Caio Carvalho; André Ricardo De Lima Damasio; Marcos Silveira Buckeridge; Rolf Alexander Prade; Fernando Segato; Maria De Lourdes Teixeira De Moraes Polizeli. 2020. "The profile secretion of Aspergillus clavatus: Different pre-treatments of sugarcane bagasse distinctly induces holocellulases for the lignocellulosic biomass conversion into sugar." Renewable Energy 165, no. : 748-757.

Journal article
Published: 20 August 2020 in Process Biochemistry
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The enzymatic lignocellulosic biomass conversion into value-added products requires the use of enzyme-rich cocktails, including β-glucosidases that hydrolyze cellobiose and cellooligosaccharides to glucose. During hydrolysis occurs accumulation of monomers causing inhibition of some enzymes; thus, glucose/xylose tolerant β-glucosidases could overcome this drawback. The search of new tolerant enzymes showing additional properties, such as high activity, wide-pH range, and thermal stability is very relevant to improve the bioprocess. We describe a novel β-glucosidase GH1 from the thermophilic Anoxybacillus thermarum (BgAt), which stood out by the robustness combination of great glucose/xylose tolerance, thermal stability, and high Vmax. The recombinant his-tagged-BgAt was overexpressed in Escherichia coli, was purified in one step, showed a high glucose/xylose tolerance, and activity stimulation (presence of 0.4 M glucose/1.0 M xylose). The optimal activity was at 65 °C - pH 7.0. BgAt presented an extraordinary temperature stability (48 h – 50 °C), and pH stability (5.5 - 8.0). The novel enzyme showed outstanding Vmax values compared to other β-glucosidases. Using p-nitrophenyl-β-D-glucopyranoside as substrate the values were Vmax (7,614 U/mg), and KM (0.360 mM). These values suffer a displacement in Vmax to 14,026 U/mg (glucose), 14,886 U/mg (xylose), and KM 0.877 mM (glucose), and 1.410 mM (xylose).

ACS Style

Paula Zaghetto de Almeida; Tássio Brito de Oliveira; Rosymar Coutinho de Lucas; José Carlos Santos Salgado; Malena Martínez Pérez; Beatriz Gálan; José Luis García; Maria De Lourdes Teixeira De Moraes Polizeli. Heterologous production and biochemical characterization of a new highly glucose tolerant GH1 β-glucosidase from Anoxybacillus thermarum. Process Biochemistry 2020, 99, 1 -8.

AMA Style

Paula Zaghetto de Almeida, Tássio Brito de Oliveira, Rosymar Coutinho de Lucas, José Carlos Santos Salgado, Malena Martínez Pérez, Beatriz Gálan, José Luis García, Maria De Lourdes Teixeira De Moraes Polizeli. Heterologous production and biochemical characterization of a new highly glucose tolerant GH1 β-glucosidase from Anoxybacillus thermarum. Process Biochemistry. 2020; 99 ():1-8.

Chicago/Turabian Style

Paula Zaghetto de Almeida; Tássio Brito de Oliveira; Rosymar Coutinho de Lucas; José Carlos Santos Salgado; Malena Martínez Pérez; Beatriz Gálan; José Luis García; Maria De Lourdes Teixeira De Moraes Polizeli. 2020. "Heterologous production and biochemical characterization of a new highly glucose tolerant GH1 β-glucosidase from Anoxybacillus thermarum." Process Biochemistry 99, no. : 1-8.

Original article
Published: 08 August 2020 in Biotechnology and Applied Biochemistry
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Aspergillus terreus can produce different holocellulose‐degrading enzymes when grown in sugarcane bagasse, with predominant pectinase activity. Thus, pectinase was selected for purification and immobilization studies. Ion exchange and molecular exclusion chromatography studies were performed, after which it was possible to semi‐purify the enzyme with a yield of 80%. The crude extract pectinase (PECEB) and the partially purified enzyme (PEC2) were immobilized on monoamino‐N‐aminoethyl (MANAE)‐agarose with pectinase activity yields of 66% and 98%, respectively. After immobilization in MANAE‐agarose, the pectinase showed higher activity at acidic pH (pH 4.0) when compared to the non‐immobilized enzyme. It was also found that after the immobilization process, there was a three‐fold improvement in the enzyme's thermostability. Also, it was possible to reuse the immobilized enzyme for up to 5 cycles of hydrolysis with effective production of reducing sugars (0.196 mg/g of substrate). The industrial application test revealed a significant decrease in the viscosity of guava juice when the immobilized enzyme was used. PECEB, immobilized on MANAE‐agarose, was the enzyme sample that generated the highest pulp viscosity reduction (approximately 47%). Although additional studies are needed for practical industrial application, the results obtained herein reveal the potential of application of immobilized pectinase in the industry. This article is protected by copyright. All rights reserved

ACS Style

Raissa Pieroni Vaz; Ana Claudia Vici; Maria De Lourdes Teixeira De Moraes Polizeli; Pérola Oliveira Magalhães; Edivaldo Ximenes Ferreira Filho. Immobilization studies of a pectinase produced by Aspergillus terreus. Biotechnology and Applied Biochemistry 2020, 68, 197 -208.

AMA Style

Raissa Pieroni Vaz, Ana Claudia Vici, Maria De Lourdes Teixeira De Moraes Polizeli, Pérola Oliveira Magalhães, Edivaldo Ximenes Ferreira Filho. Immobilization studies of a pectinase produced by Aspergillus terreus. Biotechnology and Applied Biochemistry. 2020; 68 (1):197-208.

Chicago/Turabian Style

Raissa Pieroni Vaz; Ana Claudia Vici; Maria De Lourdes Teixeira De Moraes Polizeli; Pérola Oliveira Magalhães; Edivaldo Ximenes Ferreira Filho. 2020. "Immobilization studies of a pectinase produced by Aspergillus terreus." Biotechnology and Applied Biochemistry 68, no. 1: 197-208.

Review
Published: 28 July 2020 in Molecules
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Increasing environmental and sustainability concerns, caused by current population growth, has promoted a raising utilization of renewable bio-resources for the production of materials and energy. Recently, nanocellulose (NC) has been receiving great attention due to its many attractive features such as non-toxic nature, biocompatibility, and biodegradability, associated with its mechanical properties and those related to its nanoscale, emerging as a promising material in many sectors, namely packaging, regenerative medicine, and electronics, among others. Nanofibers and nanocrystals, derived from cellulose sources, have been mainly produced by mechanical and chemical treatments; however, the use of cellulases to obtain NC attracted much attention due to their environmentally friendly character. This review presents an overview of general concepts in NC production. Especial emphasis is given to enzymatic hydrolysis processes using cellulases and the utilization of pulp and paper industry residues. Integrated process for the production of NC and other high-value products through enzymatic hydrolysis is also approached. Major challenges found in this context are discussed along with its properties, potential application, and future perspectives of the use of enzymatic hydrolysis as a pretreatment in the scale-up of NC production.

ACS Style

Michele Michelin; Daniel G. Gomes; Aloia Romaní; Maria De Lourdes T. M. Polizeli; José A. Teixeira. Nanocellulose Production: Exploring the Enzymatic Route and Residues of Pulp and Paper Industry. Molecules 2020, 25, 3411 .

AMA Style

Michele Michelin, Daniel G. Gomes, Aloia Romaní, Maria De Lourdes T. M. Polizeli, José A. Teixeira. Nanocellulose Production: Exploring the Enzymatic Route and Residues of Pulp and Paper Industry. Molecules. 2020; 25 (15):3411.

Chicago/Turabian Style

Michele Michelin; Daniel G. Gomes; Aloia Romaní; Maria De Lourdes T. M. Polizeli; José A. Teixeira. 2020. "Nanocellulose Production: Exploring the Enzymatic Route and Residues of Pulp and Paper Industry." Molecules 25, no. 15: 3411.

Original article
Published: 02 May 2020 in Biomass Conversion and Biorefinery
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The polysaccharides in the primary plant cell wall are a renewable energy source for biofuel production. However, these polysaccharides are not readily available for bioconversion, and large enzyme sets are required to deconstruct them. Here, we aimed to improve the glucan conversion using recombinant hemicellulases and esterase as a treatment in exploded and sugarcane bagasses (SCB), followed by the addition of commercial CBH I to prevent its inhibition by hemicellulases products. A high secretion level of the recombinant enzymes was observed on SDS-PAGE. The highest activities were verified at a temperature and pH ranging from 40 to 55 °C and 4.5 to 6.0, respectively. The released reducing sugar analysis showed that all enzymes act better on SCB, with xylanase C (XynC) presenting the best activity (0.54 U/mg of protein). The high-performance liquid chromatography (HPLC) analysis demonstrated that 24 h of pretreatment was enough to reach maximum glucan conversion. The best synergy was achieved between XynC and CBH I on SCB, 1.4%. All results showed that the enzymes acted better on SCB, which can be related to the biomass composition and its molecular structure. The enzymatic pretreatment of SCB with XynC was essential to improve the glucan conversion by CBH I.

ACS Style

Rosymar Coutinho De Lucas; Tássio Brito De Oliveira; Matheus Sanitá Lima; Thiago Pasin; Ana Sílvia De Almeida Scarcella; Rolf Alexander Prade; Fernando Segato; Maria De Lourdes Teixeira De Moraes Polizeli. Effect of enzymatic pretreatment of sugarcane bagasse with recombinant hemicellulases and esterase prior to the application of the cellobiohydrolase CBH I Megazyme®. Biomass Conversion and Biorefinery 2020, 1 -9.

AMA Style

Rosymar Coutinho De Lucas, Tássio Brito De Oliveira, Matheus Sanitá Lima, Thiago Pasin, Ana Sílvia De Almeida Scarcella, Rolf Alexander Prade, Fernando Segato, Maria De Lourdes Teixeira De Moraes Polizeli. Effect of enzymatic pretreatment of sugarcane bagasse with recombinant hemicellulases and esterase prior to the application of the cellobiohydrolase CBH I Megazyme®. Biomass Conversion and Biorefinery. 2020; ():1-9.

Chicago/Turabian Style

Rosymar Coutinho De Lucas; Tássio Brito De Oliveira; Matheus Sanitá Lima; Thiago Pasin; Ana Sílvia De Almeida Scarcella; Rolf Alexander Prade; Fernando Segato; Maria De Lourdes Teixeira De Moraes Polizeli. 2020. "Effect of enzymatic pretreatment of sugarcane bagasse with recombinant hemicellulases and esterase prior to the application of the cellobiohydrolase CBH I Megazyme®." Biomass Conversion and Biorefinery , no. : 1-9.

Journal article
Published: 24 April 2020 in Scientific Reports
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β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides. They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance. In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source. Kinetic characterization revealed that the MpBgl3 was highly tolerant to glucose, which is in contrast to many Bgls that are completely inhibited by glucose. A 3D model of MpBgl3 was generated by molecular modeling and used for the evaluation of structural differences with a Bgl3 that is inhibited by glucose. Taken together, our results provide new clues to understand the glucose tolerance in GH3 β-glucosidases.

ACS Style

Lummy Maria Oliveira Monteiro; Ana Claudia Vici; Matheus Pinto Pinheiro; Paulo Ricardo Heinen; Arthur Henrique Cavalcante De Oliveira; Richard John Ward; Rolf Alexander Prade; Marcos S. Buckeridge; Maria De Lourdes Teixeira De Moraes Polizeli. A Highly Glucose Tolerant ß-Glucosidase from Malbranchea pulchella (MpBg3) Enables Cellulose Saccharification. Scientific Reports 2020, 10, 1 -12.

AMA Style

Lummy Maria Oliveira Monteiro, Ana Claudia Vici, Matheus Pinto Pinheiro, Paulo Ricardo Heinen, Arthur Henrique Cavalcante De Oliveira, Richard John Ward, Rolf Alexander Prade, Marcos S. Buckeridge, Maria De Lourdes Teixeira De Moraes Polizeli. A Highly Glucose Tolerant ß-Glucosidase from Malbranchea pulchella (MpBg3) Enables Cellulose Saccharification. Scientific Reports. 2020; 10 (1):1-12.

Chicago/Turabian Style

Lummy Maria Oliveira Monteiro; Ana Claudia Vici; Matheus Pinto Pinheiro; Paulo Ricardo Heinen; Arthur Henrique Cavalcante De Oliveira; Richard John Ward; Rolf Alexander Prade; Marcos S. Buckeridge; Maria De Lourdes Teixeira De Moraes Polizeli. 2020. "A Highly Glucose Tolerant ß-Glucosidase from Malbranchea pulchella (MpBg3) Enables Cellulose Saccharification." Scientific Reports 10, no. 1: 1-12.

Original article
Published: 03 April 2020 in Molecular Ecology
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Climate change is predicted to cause more extreme events, such as heatwaves, and different precipitation patterns. The effects of warming and short‐term drought on soil microbial communities, in particular fungal communities, remain largely unexplored under field conditions. Here, we evaluated how the fungal community of a tropical grassland soil responds to these changes. A field experiment was carried out in a temperature free‐air controlled enhancement (T‐FACE) facility in Ribeirão Preto, Brazil. The isolated and combined effects of drought and a 2 °C increase in temperature were investigated. Based on metabarcoding of the ITS2 region, a total of 771 OTUs were observed. While warming affected the community structure, drought affected the alpha diversity, and the interaction between warming and drought affected both diversity and structure. The change in community composition driven by warming affected only the less abundant species (>1% of the total sequences). The most affected aspect of the fungal communities was the diversity, which was increased by drought (P<0.05), mostly by reducing the dominance of a single species, as observed in the watered plots. In a phylogenetic context, some fungal taxa were favored by changes in temperature (Hypocreales) and drought (Sordariales) or disadvantaged by both (Pleosporales). It is worth highlighting that a water deficit increased the abundance of phytopathogenic fungi, such as Curvularia, Thielavia, and Fusarium species. Overall, our results provide evidence that fungal communities in tropical grassland soils have greater sensitivity to drought than to temperature, which might increase the incidence of certain soil‐borne diseases.

ACS Style

Tássio Brito De Oliveira; Rosymar Coutinho De Lucas; Ana Sílvia De Almeida Scarcella; Alex Graça Contato; Thiago Pasin; Carlos Martinez; Maria De Lourdes Teixeira De Moraes Polizeli. Fungal communities differentially respond to warming and drought in tropical grassland soil. Molecular Ecology 2020, 29, 1550 -1559.

AMA Style

Tássio Brito De Oliveira, Rosymar Coutinho De Lucas, Ana Sílvia De Almeida Scarcella, Alex Graça Contato, Thiago Pasin, Carlos Martinez, Maria De Lourdes Teixeira De Moraes Polizeli. Fungal communities differentially respond to warming and drought in tropical grassland soil. Molecular Ecology. 2020; 29 (8):1550-1559.

Chicago/Turabian Style

Tássio Brito De Oliveira; Rosymar Coutinho De Lucas; Ana Sílvia De Almeida Scarcella; Alex Graça Contato; Thiago Pasin; Carlos Martinez; Maria De Lourdes Teixeira De Moraes Polizeli. 2020. "Fungal communities differentially respond to warming and drought in tropical grassland soil." Molecular Ecology 29, no. 8: 1550-1559.

Chapter
Published: 01 April 2020 in Biorefinery of Alternative Resources: Targeting Green Fuels and Platform Chemicals
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Bioenergy is the term used for energy produced from lignocellulosic biomass. Most of the residues produced from agricultural and industrial activities present high levels of lignocellulose. They are mostly formed by rigid structures mainly containing hemicellulose and cellulose intermixed by lignin. These macromolecules are linked by covalent and hydrogen bonds, thus forming a complex architecture, which give a great resistance to their hydrolysis. This hampers the subsequent production of fermentable sugars and their fermentation to produce second-generation bioethanol. The technologies to obtain second-generation bioethanol, independent of the plant source, involve the hydrolysis of polysaccharides from the biomass in order to generate sugars that can be fermented by yeasts. This chapter addresses the importance of biomass for the production of green fuels. In this chapter, the potential of different lignocellulosic biomasses, especially the agricultural crop residues, is described. The composition of the main molecules forming the cell wall of different plants is provided. The enzymes that are involved in the deconstruction of plant cell walls as well as the release of fermentable sugars are discussed. The pretreatment and fermentation of biomass for the second-generation ethanol production by yeasts are described. Some challenges concerning the technologies are considered, but, on the other hand, some alternatives are also pointed out.

ACS Style

Thiago Pasin; Paula Zaghetto De Almeida; Ana Sílvia De Almeida Scarcella; Juliana Da Conceição Infante; Maria De Lourdes De Teixeira De Moraes Polizeli. Bioconversion of Agro-industrial Residues to Second-Generation Bioethanol. Biorefinery of Alternative Resources: Targeting Green Fuels and Platform Chemicals 2020, 23 -47.

AMA Style

Thiago Pasin, Paula Zaghetto De Almeida, Ana Sílvia De Almeida Scarcella, Juliana Da Conceição Infante, Maria De Lourdes De Teixeira De Moraes Polizeli. Bioconversion of Agro-industrial Residues to Second-Generation Bioethanol. Biorefinery of Alternative Resources: Targeting Green Fuels and Platform Chemicals. 2020; ():23-47.

Chicago/Turabian Style

Thiago Pasin; Paula Zaghetto De Almeida; Ana Sílvia De Almeida Scarcella; Juliana Da Conceição Infante; Maria De Lourdes De Teixeira De Moraes Polizeli. 2020. "Bioconversion of Agro-industrial Residues to Second-Generation Bioethanol." Biorefinery of Alternative Resources: Targeting Green Fuels and Platform Chemicals , no. : 23-47.

Journal article
Published: 10 February 2020 in Journal of Cleaner Production
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This paper describes the obtaining of fatty acids ethyl esters from Açaí (Euterpe oleracea Martius), Buriti (Mauritia flexuosa) and spent coffee grounds oils using a Beauveria bassiana lipase expressed in Aspergillus nidulans, which gene was cloned in pExpyr vector. Carbon sources were tested to enhance recombinant lipase secretion. Cassava peels, corn meal, corn syrup, sorghum seed and wheat bran induced higher lipase activity. Monosaccharides, when combined to previously cited carbon sources, interfered at lipase production: fructose and glucose enhanced lipase production, especially on sorghum seed and wheat bran cultures; while xylose diminished its production. Lipase was immobilized on hydrophobic resins, and derivatives were characterized using pNPP transesterification with ethanol as co-substrate. Sepabead-C18 derivative demonstrated as better pNPP transesterification catalyzer, being 2-fold higher than butyl derivative. The Sepabead-C18 derivative was biochemically characterized using different temperatures and solvents. The transesterification of Açaí, Buriti and coffee spent grounds oils were performed with cyclohexane, and the product content was quantified using gas-chromatography as 17.1%, 5.0%, and 3.4%, respectively. The results demonstrated the potential of immobilized heterologous lipases to produce ethyl ester from Brazilian natural oils and a common food waste. This work will improve the enzymatic methodologies for biodiesel industry.

ACS Style

Enrico Cerioni Spiropulos Gonçalves; Malena Martínez Pérez; Ana Claudia Vici; Jose Carlos Santos Salgado; Mariana De Souza Rocha; Paula Almeida; Juliana Da Conceição Infante; Ana Sílvia De Almeida Scarcella; Rosymar Coutinho de Lucas; Andressa Tironi Vieira; Anizio Faria; Antônio Carlos Ferreira Batista; Maria De Lourdes Teixeira De Moraes Polizeli. Potential biodiesel production from Brazilian plant oils and spent coffee grounds by Beauveria bassiana lipase 1 expressed in Aspergillus nidulans A773 using different agroindustry inputs. Journal of Cleaner Production 2020, 256, 120513 .

AMA Style

Enrico Cerioni Spiropulos Gonçalves, Malena Martínez Pérez, Ana Claudia Vici, Jose Carlos Santos Salgado, Mariana De Souza Rocha, Paula Almeida, Juliana Da Conceição Infante, Ana Sílvia De Almeida Scarcella, Rosymar Coutinho de Lucas, Andressa Tironi Vieira, Anizio Faria, Antônio Carlos Ferreira Batista, Maria De Lourdes Teixeira De Moraes Polizeli. Potential biodiesel production from Brazilian plant oils and spent coffee grounds by Beauveria bassiana lipase 1 expressed in Aspergillus nidulans A773 using different agroindustry inputs. Journal of Cleaner Production. 2020; 256 ():120513.

Chicago/Turabian Style

Enrico Cerioni Spiropulos Gonçalves; Malena Martínez Pérez; Ana Claudia Vici; Jose Carlos Santos Salgado; Mariana De Souza Rocha; Paula Almeida; Juliana Da Conceição Infante; Ana Sílvia De Almeida Scarcella; Rosymar Coutinho de Lucas; Andressa Tironi Vieira; Anizio Faria; Antônio Carlos Ferreira Batista; Maria De Lourdes Teixeira De Moraes Polizeli. 2020. "Potential biodiesel production from Brazilian plant oils and spent coffee grounds by Beauveria bassiana lipase 1 expressed in Aspergillus nidulans A773 using different agroindustry inputs." Journal of Cleaner Production 256, no. : 120513.

Article
Published: 21 January 2020 in Applied Biochemistry and Biotechnology
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The use of non-potable water (such as seawater) is an attractive alternative for water intensive processes such as biomass pretreatment and saccharification steps in the production of biochemicals and biofuels. Identification and application of halotolerant enzymes compatible with high-salt conditions may reduce the energy needed for non-potable water treatment and decrease waste treatment costs. Here we present the biochemical properties of a halotolerant endo-1,4-β-xylanase produced by Aspergillus clavatus in submerged fermentation, using paper sludge (XPS) and sugarcane bagasse (XSCB), and its potential application in the hydrolysis of agroindustrial residues. The peptide mass fingerprint and amino acid sequencing of the XPS and XSCB enzymes showed primary structure similarities with an endo-1,4-β-xylanase from Aspergillus clavatus (XYNA_ASPCL). Both enzyme preparations presented good thermal stability at 50 °C and were stable over a wide range of pH and Vmax up to 2450 U/mg for XPS. XPS and XSCB were almost fully stable even after 24 h of incubation in the presence of up to 3 M NaCl, and their activity were not affected by 500 mM NaCl. Both enzyme preparations were capable of hydrolyzing paper sludge and sugarcane bagasse to release reducing sugars. These characteristics make this xylanase attractive to be used in the hydrolysis of biomass, particularly with brackish water or seawater.

ACS Style

Thiago Pasin; Jose Carlos Santos Salgado; Ana Sílvia De Almeida Scarcella; Tássio Brito De Oliveira; Rosymar Coutinho De Lucas; Mariana Cereia; José César Rosa; Richard John Ward; Marcos Silveira Buckeridge; Maria De Lourdes Teixeira De Moraes Polizeli. A Halotolerant Endo-1,4-β-Xylanase from Aspergillus clavatus with Potential Application for Agroindustrial Residues Saccharification. Applied Biochemistry and Biotechnology 2020, 191, 1111 -1126.

AMA Style

Thiago Pasin, Jose Carlos Santos Salgado, Ana Sílvia De Almeida Scarcella, Tássio Brito De Oliveira, Rosymar Coutinho De Lucas, Mariana Cereia, José César Rosa, Richard John Ward, Marcos Silveira Buckeridge, Maria De Lourdes Teixeira De Moraes Polizeli. A Halotolerant Endo-1,4-β-Xylanase from Aspergillus clavatus with Potential Application for Agroindustrial Residues Saccharification. Applied Biochemistry and Biotechnology. 2020; 191 (3):1111-1126.

Chicago/Turabian Style

Thiago Pasin; Jose Carlos Santos Salgado; Ana Sílvia De Almeida Scarcella; Tássio Brito De Oliveira; Rosymar Coutinho De Lucas; Mariana Cereia; José César Rosa; Richard John Ward; Marcos Silveira Buckeridge; Maria De Lourdes Teixeira De Moraes Polizeli. 2020. "A Halotolerant Endo-1,4-β-Xylanase from Aspergillus clavatus with Potential Application for Agroindustrial Residues Saccharification." Applied Biochemistry and Biotechnology 191, no. 3: 1111-1126.

Research paper
Published: 11 November 2019 in Bioprocess and Biosystems Engineering
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Laccases are very interesting biocatalysts of recognized importance for several industrial applications. Its production by Trametes versicolor, a white-rot fungus, was induced by a combination of cotton gin wastes (1%), a lignocellulosic waste, and vinasse (15%), an industrial by-product from sugarcane industry. The use of these agro-industrial wastes are interesting, since it helps in reducing the enzyme production costs, due to their low cost and wide availability, as well as the environmental contamination issues, due to their improper disposal. Thus, laccase production was studied in submerged fermentation of T. versicolor using these agro-industrial wastes (cotton gin waste and vinasse) as carbon source and an additional nitrogen source (0.1% peptone). Three different bioreactors were evaluated for laccase production, such as BioFlo 310 bioreactor, aluminium tray and Erlenmeyer flasks to achieve high levels of laccase production. The highest specific production of laccase was found in BioFlo 310 bioreactor with 12 days of fermentation (55.24 U/mg prot.), which has been shown to be closely related to the oxygen supply to the microorganism through aeration of the fermentation medium. This study brings new insights into green biotechnology regarding vinasse utilization, which is frequently discharged in soils, rivers, and lakes causing adverse effects on agricultural soils and biota, as well as the cotton gin waste recovery.

ACS Style

Vanessa Elisa Pinheiro; Michele Michelin; Ana Claudia Vici; Paula Almeida; Maria De Lourdes Teixeira De Moraes Polizeli. Trametes versicolor laccase production using agricultural wastes: a comparative study in Erlenmeyer flasks, bioreactor and tray. Bioprocess and Biosystems Engineering 2019, 43, 507 -514.

AMA Style

Vanessa Elisa Pinheiro, Michele Michelin, Ana Claudia Vici, Paula Almeida, Maria De Lourdes Teixeira De Moraes Polizeli. Trametes versicolor laccase production using agricultural wastes: a comparative study in Erlenmeyer flasks, bioreactor and tray. Bioprocess and Biosystems Engineering. 2019; 43 (3):507-514.

Chicago/Turabian Style

Vanessa Elisa Pinheiro; Michele Michelin; Ana Claudia Vici; Paula Almeida; Maria De Lourdes Teixeira De Moraes Polizeli. 2019. "Trametes versicolor laccase production using agricultural wastes: a comparative study in Erlenmeyer flasks, bioreactor and tray." Bioprocess and Biosystems Engineering 43, no. 3: 507-514.