UMR AGAP - Equipe Diversité, adaptation et amélioration de la vigne (DAAV)Background and Aims: Trichome('hair') density on the underside of grapevine leaves has long been used in classifying Vitis species and identifying grapevine ( Vitis vinifera L.) cultivars. Grapevine leaf trichomes, however, are of two types but few studies have examined their microanatomy and relative spatial distribution. This study addresses those aspects.Methods and Results: The anatomy and spatial distribution of erect and prostrate trichomes on abaxial surfaces of leaves of 13 V. vinifera cultivars were examined microscopically. Erect trichomes are conical, ca. 300 mu m long. Prostrate trichomes are cylindrical, ca. 2000 mu m long, becoming senescent and flattened as leaves age. The relative abundance and spatial density of each trichome type varies among grapevine cultivars. When both types become entangled they form a 'hair coat', ca. 1.5 times thicker than the leaf lamina, supported by erect trichomes.Conclusions: Grape leaf trichomes are non-glandular, uniseriate structures of two distinct types, 'erect' and 'prostrate'. Both types arise from the epidermis but both the arrangement of their basal cells forming the point of attachment and their fluorescent properties differ markedly.Significance of the Study: The microanatomical features and fluorescent characteristics of grape leaf trichomes may facilitate improved ampelographic discrimination of grapevine cultivars

The ‘Narcea rose’ is a recently described yet ancient rose cultivar of interest to the perfume industry. Given its excellent adaptation to the conditions of the place where it was rediscovered, the possibilities of its horticultural/industrial production have been under examination for some time. The hydrodistillation process produces a red-to-brownish mixture of water and rose petals that could contain compounds that could be used in other industrial procedures. Their recovery and further utilization would reduce disposal costs and improve the sustainability of relevant industries. This work reports the quantification, by high-performance liquid chromatography (HPLC–MS) and quadrupole time of flight Q-TOF analyses, of the polyphenol content in the waste water. This waste was found to contain high concentrations of quercetin, gallic acid and ellagic acid, as well as smaller concentrations of kaempferol and its derivatives, all of which can influence plant, human and animal health.

Prostate (PC) and breast cancer (BC) are heterogeneous hormonal cancers. Treatment resistance and adverse effects are the main limitations of conventional chemotherapy treatment. The use of sensitizing agents could improve the effectiveness of chemotherapeutic drugs as well as obviate these limitations. This study analyzes the effect of single catechin (CAT), procyanidin B2 (ProB2) treatment as well as the co-adjuvant treatment of each of these compounds with docetaxel (DOCE). We used PC- and BC-derived cell lines (PC3, DU-145, T47D, MCF-7 and MDA-MB-231). The short and long-term pro-apoptotic, anti-proliferative and anti-migratory effects were analyzed. RT-qPCR was used to discover molecular bases of the therapeutic efficacy of these compounds. ProB2 treatment induced a two- to five-fold increase in anti-proliferative and pro-apoptotic effects compared to single DOCE treatment, and also had a more sensitizing effect than DOCE on DU145 cells. Regarding BC cells, ProB2- and CAT-mediated sensitization to DOCE anti-proliferative and pro-apoptotic effects was cell-independent and cell-dependent, respectively. Combined treatment led to high-efficacy effects on MCF-7 cells, which were associated to the up-regulation of CDKN1A, BAX, caspase 9 and E-cadherin mRNA under combined treatment compared to single DOCE treatment. CAT and ProB2 can enhance the efficacy of DOCE therapy on PC and BC cells by the sensitizing mechanism.

Olives (Olea europaea L.) are an important crop in the Mediterranean Basin, but it is not well-known that they have also been grown in other areas, such as Galicia in northwestern Spain. Although commercial production ended long ago in this peripheral growing region, it remains home to olive resources that are well-adapted to the prevailing environmental conditions, providing a valuable but largely undocumented source of genetic variation. Following a survey of Galicia to locate examples of centuries-old olive trees, those detected were subjected to molecular characterization using a set of microsatellite markers, as well as full botanical characterization using the features established by the International Union for the Protection of New Varieties of Plants, along with others proposed by the present authors. These procedures allowed 11 undescribed varieties to be identified, which are new genetic resources that might be of use in olive improvement programs or studies of how the species adapts to different climates. The trees also underwent preliminary health checks, allowing disease-free specimens of each variety to be propagated. The addition of this material to the Community Plant Variety Office’s register of commercial varieties is underway.

The present work reports the discovery and the complete characterisation of an ancient cultivated rose variety found growing in a private garden in the southwest of the Principality of Asturias (northern Spain). The variety is here given the name Narcea. The majority of roses currently cultivated belong to the so-called group of ‘Modern Roses’, all of which were obtained after 1867 via artificial crosses and improvement programmes. All are destined for ornamental use. Until the 19th century, the great majority of the many ancient cultivated roses in Europe were used in perfumery and cosmetics, or had medicinal uses.Rosa damascenaandRosa centifolliaare still grown and used by the French and Bulgarian perfume industries. The Asturian Massif of the Cantabrian Mountain Range provides a natural habitat for some 75% of the wild members of the genusRosa, but until now there was no evidence that this area was home to ancient cultivated roses. A complete botanical description is here provided for a discovered ancient rose. It is also characterised according to a series of sequence tagged microsatellite sites, and its agronomic features are reported. In addition, a histological description (optical and scanning electronic microscope studies) of the petals is offered, along with an analysis of the volatile compounds present in these organs as determined by solid phase microextraction and gas chromatography-mass spectroscopy. The results reveal the uniqueness of this ancient type of rose and suggest it may be of interest to the perfume industry.

Grenache Noir and Syrah are two of the grapevine ( Vitis vinifera L.) cultivars used to a great extent worldwide. They have very different leaf morphologies from an ampelographic (botanical) point of view. This might also be related to differences in the anatomy and micro-morphology of their leaves. The goal of the present work was to compare these cultivars’ leaf anatomy and morphology. Adult leaves from both cultivars were characterised using a range of microscopy techniques. Grenache Noir had a significantly smaller leaf surface area, but a significantly thicker leaf blade, than Syrah. It also had significantly larger stomata and a larger stomatal index than Syrah. The distribution of mesophyll tissues was similar in both cultivars, but the upper epidermis was significantly thicker in Grenache Noir, and the palisade parenchyma cells were longer in Syrah. The mesophyll tissues of both cultivars contained abundant idioblasts carrying crystals of calcium oxalate and mucilage. This work reveals quantitative and qualitative differences in the anatomy and morphology of mature Grenache Noir and Syrah leaves. Further work is needed to determine how these anatomical and morphological differences may be connected with different responses at the functional level.