The study aimed to determine the biochemical reference ranges for lambs from birth to 1 year of age in the tropics. Data were obtained from experiments performed in several institutions and commercial farms using Santa Inês, Dorper, Lacaune, Morada Nova, Bergamacia and Suffolk lambs reared under different conditions (grazing, feedlot, semi-feedlot) from 2006 to 2017. Serum energy-, protein-, mineral-, and enzyme-related metabolites were evaluated. The metabolic energy profile included data from glucose, cholesterol, triglycerides, fructosamine, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and very-low-density lipoprotein cholesterol; the metabolic protein profile included total protein, uric acid, urea, albumin, and creatinine; the metabolic mineral profile included data on calcium, phosphorus, and magnesium; and the metabolic enzymatic profile included the enzymes aspartate aminotransferase, gamma-glutamyl transferase, alkaline phosphatase, and creatine kinase. The reference ranges were estimated using confidence intervals with a 95% confidence level, and percentiles were estimated or bootstrapped nonparametrically when the data were not normally distributed. The serum biochemical reference ranges determined for lambs are strongly divergent from those established by one of the most cited books on the topic, especially considering the high serum urea and cholesterol concentrations and low levels of blood glucose observed. Therefore, the serum biochemical reference ranges for lambs from birth to 1 year of age in the tropics differ from international data, which consider adult sheep in temperate climate zones.

Late pregnancy is accompanied by dramatic changes in the metabolism of sheep, in which the nutrient requirements increase and the metabolic capacity of ewes is under severe stress due to the rapid foetal growth and development of the mammary gland. The blood metabolic profile can be used to monitor these alterations, which can lead to metabolic disorders such as pregnancy toxaemia. However, data available on serum parameters in sheep do not consider physiological state. Therefore, the present study aimed to determine the biochemical reference ranges for pregnant ewes, including serum energy, protein and enzyme-related metabolites. Data from a variety of metabolites were obtained from experiments performed in several institutions and commercial farms using Santa Inês, Dorper, Lacaune, Morada Nova, Bergamacia and Suffolk ewes reared under different conditions (grazing, feedlot, semi-feedlot, collective and/or individual pens, and metabolic cages) from 2006 to 2017. All animals were healthy and without feed restriction. Data from ewes with any clinical manifestations were removed. The metabolic energy profile included data of glucose, cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein and very-low-density lipoprotein levels; the metabolic protein profile included the metabolites total protein, uric acid, urea, albumin, and creatinine; and the metabolic enzymatic profile included the enzymes aspartate aminotransferase, gamma-glutamyl transferase and alkaline phosphatase. The reference ranges were estimated using the software RefVal 4.11. Dixon’s test was used to identify and remove outliers. The confidence intervals and percentiles were estimated using the nonparametric method of bootstrap when data were not normally distributed. A 95% confidence level was used. The serum biochemical reference ranges for pregnant sheep determined in our study were strongly divergent from those established by one of the most cited books on the topic, especially considering the high serum urea and cholesterol concentrations and low levels of blood glucose observed. Therefore, it is essential to consider physiological status when evaluating the blood metabolic profile of pregnant ewes in order to maintain an adequate nutritional management and to prevent health disorders that may lead to productive and reproductive losses.

O trabalho teve como objetivo definir intervalos de referência para valores de metabólitos energéticos, proteicos e enzimáticos em ovinos. Os dados utilizados foram oriundos de vários experimentos realizados entre 2006 e 2018, em diversas instituições de ensino superior e pesquisa. Foi utilizado banco de dados diversificado com observações de ovinos de diferentes raças, genótipos, idades e categorias fisiológicas, criados em diferentes sistemas de manejo. Todos os animais utilizados eram saudáveis e dados de animais que apresentaram quaisquer manifestações clínicas foram descartados. Para determinar o perfil metabólico energético foram obtidos dados de glicose, colesterol, triglicerídeos, frutosamina, HDL (lipoproteína de alta densidade), LDL (lipoproteína de baixa densidade) e VLDL (lipoproteína de muito baixa densidade); para o perfil proteico, dados de proteínas totais, ácido úrico, ureia, albumina e creatinina; e para o enzimático, dados de AST (aspartato aminotransferase), GGT (gama glutamil transferase) e FA (fosfatase alcalina). As análises laboratoriais foram realizadas nos aparelhos Analisador bioquímico semiautomático modelo BIO - 2000 da marca Bioplus© e Analisador automático de bioquímica modelo PKL-125 da marca MHLab®. Para a estimativa e determinação dos valores de referência, foi utilizado programa RefVal 4.11, sendo os valores outliers removidos da avaliação. Após a determinação dos valores de referência comparou-se os dados obtidos com tabelas internacionais estabelecidas na literatura, sendo possível observar que os intervalos encontrados neste trabalho foram mais amplos, abrangendo melhor resultados obtidos em experimentos realizados no Brasil.

Two studies evaluated the productive characteristics of young Nellore heifers receiving different days on feed (DOF) to determine the biological slaughter endpoint. In Experiment 1 (Exp. 1), fifty-one Nellore heifers [324 ± 19.3 kg of body weight (BW); 16 ± 1 months] were split into three DOF lengths (45, 75, or 105 days), while in Experiment 2 (Exp. 2), thirty-six Nellore heifers (362 ± 25.5 kg of BW; 18 ± 1 months of age) were split into three DOF lengths (45, 90, or 135 days). In both studies, all animals were distributed in complete randomized blocks according to initial BW and stratified via carcass ultrasound. The diet was supplied ad libitum, allowing 3% of refusals. The point at which the animals achieved 25% of ether extract of shrunk body weight (EE_SBW) was defined as the biological endpoint. Thus, relationships were made between some characteristics obtained in both studies. Positive linear relationships were found between backfat thickness (BFT) vs. EE_SBW (p < 0.001, r = 0.84) and BFT vs. body weight (p < 0.001, r = 0.77). Our results suggest that the biological slaughter endpoint for young Nellore heifers is 6.97 mm of backfat thickness or 402 kg shrunk body weight, corresponding to around 75 DOF.

This study evaluated different herbage allowances from mid to late pregnancy on pre- and postpartum physiological responses, milk production, and the performance of Nellore cows and the preweaning growth of their female offspring. Sixty multiparous Nellore cows were blocked by their body weight (BW; 425 ± 36 kg) and body condition score (BCS; 3.67 ± 0.23, scale 1–5) and randomly allocated to twelve pastures. Treatments consisted of two different herbage allowances (HA) during pregnancy: low HA (LHA; 2.80 kg DM/kg of BW) and high HA (HHA; 7.60 kg DM/kg of BW). Both treatment groups were fed 1 g/kg BW of a protein supplement. After calving, all cow–calf pairs were combined in a single group. The effects of maternal treatment × day of the study were detected for herbage mass and allowance, the stocking rate and forage crude protein, and for cow BW, BCS, and carcass measures (p < 0.01). Milk yield corrected to 4% fat, while the levels of fat total solids and cow plasma IGF-1 and urea were different (p ≤ 0.04) between treatments. HHA offspring was heavier (p ≤ 0.05) at 120 days and at weaning. A high herbage allowance can be implemented from mid-gestation until calving to increase cow prepartum performance, post-partum milk yield and composition, and positively modulate female offspring preweaning growth.

The objective of this study was to evaluate the effect of adding exogenous enzymes to the diet on nutrient intake and digestibility, nitrogen balance, ingestive behavior and rumen movement of ewe lambs. Five ewe lambs, Dorper x Santa Inês crossbred, with an average age of 7 ± 1 months, average weight of 36.40 ± 2.36 kg were assigned in a 5x5 Latin square design. The treatments consisted of adding exogenous enzymes to the concentrate: Allzyme, Fibrozyme®, Amaize®, Mix and Control. Analyses of variance were applied and means were compared by the SNK test, and non-parametric Kruskal Wallis test at 5% significance. The dry matter intake in relation to body weight, crude protein intake and nitrogen intake were higher with the use of amylolytic enzyme compared to the other treatments (p <0.05). Nitrogen balance was higher with the use of amylolytic enzymes and the Allzyme® enzyme complex (p <0.05). A longer time spent in total chewing was observed with the inclusion of fibrolytic and amylolytic enzymes without changes in rumen movement. The use of exogenous enzymes promotes better use of nutrients, with high digestibility of dry matter, neutral detergent fiber and crude protein.