Groundwater is considered as good alternative to potable water because of its low turbidity and perceived low contamination. The study assessed the physio-chemical and heavy metals concentrations in eight randomly selected boreholes water at Muledane village in Limpopo Province of South Africa and the results were compared with South African National standard permissible limit. The impacts of heavy metals on human health was further determined by performing quantitative risk assessment through ingestion and dermal adsorption of heavy metals separately for adults and children in order to estimate the magnitude of heavy metals in the borehole samples. Parameters such as turbidity, nitrate, iron, manganese and chromium in some investigated boreholes did not comply with standard limits sets for domestic water use. Multivariate analyses using principal component analysis and hierarchical cluster analysis revealed natural and anthropogenic activities as sources of heavy metal contamination in the borehole water samples. The calculated non-carcinogenic effects using hazard quotient toxicity potential, cumulative hazard index and chronic daily intake of groundwater through ingestion and dermal adsorption pathways were less than a unity, which showed that consumption of the water could pose little or no significant health risk. However, maximum estimated values for an individual exceeded the risk limit of 10−6 and 10−4 with the highest estimated carcinogenic exposure risk (CRing) for Cr and Pb in the groundwater. This could pose potential health risk to both adults and children in the investigated area. Therefore, precaution needs to be taken to avoid potential CRing of people in Muledane area especially, children using the borehole water.

Enteric viruses are common waterborne pathogens found in environmental water bodies contaminated with either raw or partially treated sewage discharge. Examples of these viruses include adenovirus, rotavirus, noroviruses, and other caliciviruses and enteroviruses like coxsackievirus and polioviruses. They have been linked with gastroenteritis, while some enteric viruses have also been implicated in more severe infections such as encephalitis, meningitis, hepatitis (hepatitis A and E viruses), cancer (polyomavirus), and myocarditis (enteroviruses). Therefore, this review presents information on the occurrence of enteric viruses of public health importance, diseases associated with human exposure to enteric viruses, assessment of their presence in contaminated water, and their removal in water and wastewater sources. In order to prevent illnesses associated with human exposure to viral contaminated water, we suggest the regular viral monitoring of treated wastewater before discharging it into the environment. Furthermore, we highlight the need for more research to focus on the development of more holistic disinfection methods that will inactivate waterborne viruses in municipal wastewater discharges, as this is highly needed to curtail the public health effects of human exposure to contaminated water. Moreover, such a method must be devoid of disinfection by-products that have mutagenic and carcinogenic potential.

Water contamination is a global health problem, and the need for safe water is ever-growing due to the public health implications of unsafe water. Contaminated water could contain pathogenic bacteria, protozoa, and viruses that are implicated in several debilitating human diseases. The prevalence and survival of waterborne viruses differ from bacteria and other waterborne microorganisms. In addition, viruses are responsible for more severe waterborne diseases such as gastroenteritis, myocarditis, and encephalitis among others, hence the need for dedicated attention to viral inactivation. Disinfection is vital to water treatment because it removes pathogens, including viruses. The commonly used methods and techniques of disinfection for viral inactivation in water comprise physical disinfection such as membrane filtration, ultraviolet (UV) irradiation, and conventional chemical processes such as chlorine, monochloramine, chlorine dioxide, and ozone among others. However, the production of disinfection by-products (DBPs) that accompanies chemical methods of disinfection is an issue of great concern due to the increase in the risks of harm to humans, for example, the development of cancer of the bladder and adverse reproductive outcomes. Therefore, this review examines the conventional disinfection approaches alongside emerging disinfection technologies, such as photocatalytic disinfection, cavitation, and electrochemical disinfection. Moreover, the merits, limitations, and log reduction values (LRVs) of the different disinfection methods discussed were compared concerning virus removal efficiency. Future research needs to merge single disinfection techniques into one to achieve improved viral disinfection, and the development of medicinal plant-based materials as disinfectants due to their antimicrobial and safety benefits to avoid toxicity is also highlighted.

Anaerobic digestion (AD) technology has become popular and is widely used due to its ability to produce renewable energy from wastes. The bioenergy produced in anaerobic digesters could be directly used as fuel, thereby reducing the release of biogas to the atmosphere. Due to the limited knowledge on the different process disturbances and microbial composition that are vital for the efficient operation of AD systems, models and control strategies with respect to external influences are needed without wasting time and resources. Different simple and complex mechanistic and data-driven modeling approaches have been developed to describe the processes taking place in the AD system. Microbial activities have been incorporated in some of these models to serve as a predictive tool in biological processes. The flexibility and power of computational intelligence of evolutionary algorithms (EAs) as direct search algorithms to solve multiobjective problems and generate Pareto-optimal solutions have also been exploited. Thus, this paper reviews state-of-the-art models based on the computational optimization methods for renewable and sustainable energy optimization. This paper discusses the different types of model approaches to enhance AD processes for bioenergy generation. The optimization and control strategies using EAs for advanced reactor performance and biogas production are highlighted. This information would be of interest to a dynamic group of researchers, including microbiologists and process engineers, thereby offering the latest research advances and importance of AD technology in the production of renewable energy.