Surface modification of polydimethylsiloxane (PDMS) for organ‐on‐a‐chip (OOC) systems is fundamental for the success of cell physiological assays. Although UV light is commonly used for this purpose, surface chemical modifications are only temporary. To overcome these limitations, an alternative approach is proposed: a physicochemical modification using ozone and the heterofunctional crosslinker sulfo‐SANPAH (SS). This simple one‐step approach is carried out on PDMS microchannels of OOC platforms. A broad physicochemical characterization based on spectroscopy and microscopy techniques confirms successful modification of the PDMS surface. The PDMS surface contact angle decreases after addition of SS (from 101.4° to 79.9°) but decreases as low as 20.8° after extracellular matrix deposition, demonstrating successful transition of the surface from hydrophobic to hydrophilic. For biological characterization, human liver sinusoidal endothelial cells (LSEC) and murine primary hepatocytes are cultured in OOC channels for up to 7 days. LSECs and hepatocytes establish strong cell adhesions under laminar flow and form a well‐established canaliculi network, respectively. This one‐step method of PDMS surface modification for OOC is simpler than others reported in the literature (e.g., based on 3‐aminopropyltriethoxysilane) and ideal for beginners interested in using microfluidic platforms for cell culture.

In this work, we present an accessible benchtop fabrication technique to obtain a planar array of gold nanowrinkled surface electrodes (ANSE) for the construction of electrochemical cells, specifically to monitor soluble biomarkers of interest in cell culture environments. We present a complete characterization of the array and its response as an electrochemical cell. To validate our sensor, we evaluated the device sensitivity to detect nitric oxide (NO), an important molecule produced by endothelial cells as a response to environmental signals such as mechanics and growth factors. While testing measurements of nitric oxide in aqueous solutions with isotonic salt concentrations, we evidenced the influence of the environmental conditions for such electrochemical measurements, showing that the aqueous medium, usually not accounted for, significantly impacts the outcome. Finally, we present the application of the electrochemical sensor for the detection of nitric oxide released from stimulated endothelial cells as a proof of concept.

Two-photon polymerization (2PP) enables the fabrication of complex three dimensional microstructures with submicron precision, making it a powerful tool for mechanobiology studies. In this work we present a highly reproducible 2PP- based method to fabricate high aspect ratio micropillars, achieving aspect ratios up to 1:20 while maintaining structural stability. These micropillars were employed to quantify the contractile forces exerted by pituitary pericytes in response to ATP stimulation, providing novel insights into their mechanobiology. Using a custom-designed perfusion chamber and confocal microscopy, we measured both intracellular calcium dynamics and pillar displacements, revealing that pericytes generate contractile forces primarily through their peripheral extensions, with forces reaching up to 77.81 ± 26.85 nN post-stimulation. The study also introduces a novel protocol for isolating and culturing primary pituitary pericytes, confirming their identity through immunostaining for PDGFR-β and NG2 markers. Our results demonstrate that pericytes exhibit a delayed but sustained contractile response to ATP, correlating with intracellular calcium signaling. This work advances our understanding of pericyte mechanotransduction and their role in microvascular regulation, offering a robust platform for future mechanobiology research.