The goal of this work is to develop a sustainable value chain of carbonaceous adsorbents that can be produced from the solid fibrous digestate (SFD) of biogas plants and further applied in integrated desulfurization-upgrading (CO₂/CH₄ separation) processes of biogas to yield high-purity biomethane. For this purpose, physical and chemical activation of the SFD-derived BC was optimized to afford micro-mesoporous activated carbons (ACs) of high BET surface area (590–2300 m²g⁻¹) and enhanced pore volume (0.57–1.0 cm³g⁻¹). Gas breakthrough experiments from fixed bed columns of the obtained ACs, using real biogas mixture as feedstock, unveiled that the physical and chemical activation led to different types of ACs, which were sufficient for biogas upgrade and biogas desulfurization, respectively. Performing breakthrough experiments at three temperatures close to ambient, it was possible to define the optimum conditions for enhanced H₂S/CO₂ separation. It was also concluded that the H₂S adsorption capacity was significantly affected by the restriction to gas diffusion. Hence, the best performance was obtained at 50 °C, and the maximum observed in the H₂S adsorption capacity vs. the temperature was attributed to the counterbalance between adsorption and diffusion processes.

Ammonia and sulfide derived from the reduction of sulfate by the sulfate-reducing bacteria (SRB) are two of the most common inhibitors in anaerobic digestion. Zeolites and bentonites are characterized as porous materials able to adsorb both ammonia and sulfur compounds and seem to be promising candidates as additives in anaerobic digestion to counteract this co-inhibition. In this study, bentonite and zeolite 13X were subjected to alkali modification at different concentrations of NaOH to alter their physicochemical properties, and their effect on the alleviation of ammonia and sulfate co-inhibition in anaerobic digestion of cow manure was examined. The methane production in 13X treatments (13X without NaOH, 13X02-NaOH 0.2 M and 13X1-NaOH 1 M) was elevated by increasing the NaOH concentration in the modification step, resulting in a significance increase by 8.96%, 11.0% and 15.56% in 13X treatments compared to the treatment without additive. Bentonite treatments did not show the same behavior on the toxicity mitigation. The results appear to be influenced by the combined effect of 13X zeolites on the sulfur compounds adsorption and on the increase in pH and Na⁺ concentration in the batch reactors.

The market for plant-based proteins, particularly alternatives to dairy products, is growing due to health benefits and environmental concerns. Many people are adopting plant-based diets, due to lactose intolerance, vegan and vegetarian lifestyles; thus, promoting lactose-free and cholesterol-free alternatives to milk-derived products such as ice cream. Rice, which is a lactose and gluten free product, as well as low in fat, and calories, could be a promising substitute for dairy milk in foods such as ice cream. In the current study, the development of a novel recipe for ice cream based on brown rice milk was investigated. After standardizing the rice milk production protocol, six combinations of hydrocolloids were used as emulsifiers, as well as four sweeteners for granulated sugar substitution were tested. In all formulations, meltdown and overrun parameters were measured and at the final stage organoleptic evaluation was conducted to determine the level of sensory panel acceptance. In conclusion, it was evident that the rice milk-based ice cream, which combines xanthan gum, guar gum, k-carrageenan, with 30% sugar substitution with stevia, was the formulation with the highest acceptance in the physiological characteristics and sensory evaluation.

The current study investigated the performance of a moving bed biofilm reactor (MBBR), when adding 3D-printed biocarriers fabricated with 13X and bentonite (MBBR 3D), when using K1 commercial biocarriers (MBBR K1) and when not adding biocarriers at all (control MBBR). For the evaluation of the MBBR efficiency, various physicochemical parameters were measured, while biofilm extracted from the biocarriers was evaluated. The findings suggest that there is an optimal biodegradation of the organic load in all MBBR units. The nitrification and denitrification processes were improved in MBBR 3D as compared to the control MBBR and MBBR K1. The dry mass of the biofilm in the 3D-printed biocarriers was two orders of magnitude larger than in the K1 biocarriers. Moreover, in the K1 biocarriers the mass of the biofilm varied in relation to time, since it could not be protected inside the holes, something that did not happen with the 3D-printed biocarriers. Finally, it was found, mostly in MBBR 3D and less in MBBR K1, that the growth of nitrifying bacteria and heterotrophs inside the units increased the biomass production in the form of soluble microbial products, which in turn favored the adhesion of biomass on the surface of biocarriers.

Lameness is one of the most significant problems in cattle breeding. It is a major factor that causes discomfort and significantly reduces the welfare of affected animals. Lameness can result in a decrease in milk production or, if not detected early enough, may require the animal to be culled, leading to severe direct and indirect economic consequences for the business. The delayed recognition of lameness is often due to the methods used for detection, which mainly rely on the observation of animal mobility by the breeder. These methods almost exclude the early detection of the problem. This work aims to establish a new detection system that will be able to identify on time, reliably, and at an early stage the lameness symptoms based on the movement parameters of the animals.

In the present work, a comparative evaluation of an MBBR unit performance was carried out for the following cases: when adding 3D-printed biocarriers fabricated with 13X and bentonite, when using K1 commercial biocarriers and when not adding biocarriers at all. For the evaluation of the MBBR efficiency, various physicochemical parameters were measured, while static light scattering and optical microscopy observations were additionally used. Finally, biofilm extracted from the biocarriers was evaluated. The findings suggest that there is an optimal biodegradation of the organic load in all MBBR units. The nitrification and denitrification process were improved at the 3D MBBR as compared to the control MBBR and MBBR K1. The dry mass of the biofilm in the 3D-printed biocarriers was two orders of magnitude larger than the one in the K1 biocarriers. What is more, in the K1 biocarriers the mass of the biofilm varied in relation to time, due to the fact that it could not be kept inside the holes, something that was not observed to happen with the 3D-printed biocarriers. Finally, it was observed, mostly in the 3D MBBR and less in the K1 MBBR, that the growth of nitrifying bacteria and heterotrophs inside the units increased the biomass production in the form of SMP, which in turn favored the adhesion of biomass on the surface of biocarriers.