In the present study, we isolated a trypsin-producing strain DMN6 from the leather waste and identified it as Bacillus licheniformis through a two-step screening strategy. The trypsin activity was increased up to 140 from 20 U/mL through culture optimization. The enzyme was purified to electrophoretic homogeneity with a molecular mass of 44 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the specific activity of purified enzyme is 350 U/mg with Nα-Benzoyl-l-arginine ethylester as the substrate. The optimum temperature and pH for the trypsin are 65 °C and pH 9.0, respectively. Also, the enzyme can be significantly activated by Ba²⁺. This enzyme is relatively stable in alkaline environment and displays excellent activity at low temperatures. It could retain over 95% of enzyme activity after 180 min of incubation at 45 °C. The distinguished activity under low temperature and prominent stability enhance its catalytic potential. In the current work, the open reading frame was obtained with a length of 1371 nucleotides that encoded a protein of 456 amino acids. These data would warrant the B. licheniformis trypsin as a promising candidate for catalytic application in collagen preparation and leather bating through further protein engineering.

To improve environmental quality in enclosed piggeries, a monitoring and control system was designed based on a track inspection robot. The system includes a track mobile monitoring platform, an environmental control system, and a monitor terminal. The track mobile monitoring platform consists of three main components: a single-track motion device, a main box containing electronic components, and an environmental sampling device. It is capable of detecting various environmental parameters such as temperature, humidity, NH₃ concentration, CO₂ concentration, light intensity, H₂S concentration, dust concentration, and wind speed at different heights below the track. Additionally, it can control on-site environmental control equipment such as lighting systems, ventilation systems, temperature control systems, and manure cleaning systems. The networked terminal devices enable real-time monitoring of field equipment operating status. An adaptive fuzzy PID control algorithm is embedded in the system to regulate the temperature of the piggery. Field tests conducted on a closed nursery piggery revealed that the system effectively controlled the maximum temperature range within 2 °C. The concentrations of CO₂, NH₃, and PM2.5 were maintained at a maximum of 1092 mg∙m⁻³, 16.8 mg∙m⁻³, and 35 μg∙m⁻³, respectively. The light intensity ranged from 51 to 57 Lux, while the wind speed remained stable at approximately 0.35 m∙s⁻¹. The H₂S concentration was significantly lower than the standard value, and the lowest relative humidity recorded was 18% RH at high temperatures. Regular humidification is required in closed piggeries and other breeding places when the system does not trigger the wet curtain humidification and cooling function, as the relative humidity is lower than the standard value. By controlling the temperature, the system combined with a humidification device can meet environmental requirements. The control method is simple and effective, with a wide range of applications, and holds great potential in the field of agricultural environmental control.