Background and Aim: In the past, the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) infections in both humans and animals has increased across Thailand. Staphylococcus argenteus has been associated with infections among humans, exotic pets, and livestock. Both species have been identified in non-human primate species from geographically diverse locations but not from non-human primates in Thailand. This study aimed to determine the presence of MRSA/ methicillin-susceptible S. aureus (MSSA) and S. argenteus isolates collected from buccal swab samples in Macaca fascicularis at Kosumpee Forest Park (KFP), Maha Sarakham, Northeast Thailand. Materials and Methods: Aseptic buccal swab samples were collected from 30 free-ranging macaques in November 2018. All isolates were tested using multiple biochemical tests and S. aureus latex slide agglutination test. Presumptive S. aureus isolates were tested for the presence of the mecA gene using polymerase chain reaction (PCR) assays. The isolates were phenotypically determined to be resistant to a β-lactam antibiotic using the disk diffusion method with a 30 μg cefoxitin disk. The isolates were analyzed by PCR for the non-ribosomal peptide synthetase (NRPS) gene to distinguish S. argenteus from S. aureus. Results: Fifteen macaques (50%) were colonized with S. aureus and 21 isolates were characterized. Three of the macaques carried both the MRSA and MSSA isolate. One animal carried both MRSA and S. argenteus isolate, and one animal carried only S. argenteus. The NRPS gene analysis confirmed that 2 isolates (9.52%) were S. argenteus and 19 isolates (90.48%) were S. aureus [five MSSA and 14 MRSA]. Conclusion: This study is the first to identify MRSA/MSSA and S. argenteus in wild free-ranging M. fascicularis from Thailand at the KFP in Maha Sarakham. This study is also the first report on the occurrence of S. argenteus carriage in M. fascicularis from Thailand.

Microplastic pollution is a global concern arising from the extensive production and use of plastics. The prevalence of microplastics (MPs) in the environment is escalating due in large part to the excessive use of plastics in various human-related activities. Consequently, animals are being exposed to MPs through dietary intake, which poses significant health risks to the wild populations. The objective of the study was to assess the concentration of MPs in the feces of wild long-tailed macaques (Macaca fascicularis) in the Kosumpee Forest Park (KFP) located in Northeast Thailand. KFP is situated in close proximity to the town of Kosum Phisai and experiences considerable human–primate interaction. Fresh fecal drops from 50 adult macaques were collected and sampled. MP presence in the feces was measured using density separation through visual identification under a stereomicroscope. We found a total of 396 MP particles in the feces with an average of 7.9 particles/macaque. Two forms of MPs were found in the macaques’ feces including fibers (391 pieces; 98.73%) and asymmetric fragments (5 pieces; 1.27%), with sizes mostly ranging under 1000 µm. The most observed color of MPs was blue (152 pieces; 38.48%). This study highlights the impact of anthropogenic waste and the potential health problems that can be caused to wild animals via microplastic pollution. The results contribute to the ongoing discussions on environmental health within the One Health framework.

The Staphylococcus (S.) aureus complex, including methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA), and S. argenteus are bacterial pathogens that are responsible for both human and animal infection. However, insights into the molecular characteristics of MRSA, MSSA, and S. argenteus carriages in wildlife, especially in long-tailed macaques, rarely have been reported in Thailand. The objective of this study was to assess molecular characterization of MRSA, MSSA, and S. argenteus strains isolated from free-ranging long-tailed macaques (Macaca fascicularis) at Kosumpee Forest Park, Maha Sarakham, Thailand. A total of 21 secondary bacterial isolates (including 14 MRSA, 5 MSSA, and 2 S. argenteus) obtained from the buccal mucosa of 17 macaques were analysed by a Polymerase chain reaction (PCR) to identify several virulence genes, including pvl, tst, hla, hlb clfA, spa (x-region), spa (IgG biding region), and coa. The most prevalent virulence genes were clfA, coa, and the spa IgG biding region which presented in all isolates. These data indicated that MRSA, MSSA, and S. argenteus isolates from the wild macaques at Kosumpee Forest Park possess a unique molecular profile, harbouring high numbers of virulence genes. These findings suggest that wild macaques may potentially serve as carriers for distribution of virulent staphylococcal bacteria in the study area.

The life cycle and larval diet of Amphipsyche meridiana Ulmer 1902 were investigated in a natural freshwater habitat at Kasetsart University's Kamphaeng Saen Campus in Nakhon Pathom Province, Thailand. Larvae were collected monthly from December 2021 to November 2022. Most species of Hydropsychidae have five larval instars. The first to fifth larval instars were found in the stream throughout the year. Head capsule widths of the first to fifth instar larvae were 0.1–0.3, 0.4–0.6, 0.7–0.9, 1.0–1.2, and 1.3–1.6 millimeters, respectively. The highest number of larvae was found in July 2022. Adult emergence occurred throughout the year, with the highest number occurring in April 2022. A. meridiana's life cycle was characterized as multivoltine, with year-round recruitment. The Canonical Correspondence Analysis (CCA) was used to determine how conditions in the environment influenced larval abundance. The factors influencing the amounts of larvae are water depth, water temperature, pH, dissolved oxygen, ammonia-nitrogen, and sulfate. The larvae of this species are omnivorous filterers. The main food types found in the gut were blue-green algae and green algae, comprising 34.11% and 33.07% of its ingestion. In addition, the digestive tract was abundant with diatoms, rotifers, and insect fragments, accounting for 32.82% of the diet.

Background: Leptospirosis is a zoonotic disease that is ubiquitously distributed and is classified as a re-emerging infectious disease in humans and animals. Many serovars are carried by wildlife; all of them are capable of causing illness in humans. The purpose of this study was to investigate the prevalence of Leptospirosis in wild long-tailed macaques (Macaca fascicularis) at Kosumpee Forest Park, Mahasarakham, Thailand. Methods: A cross-sectional study was conducted at the park. Blood samples were collected via saphenous vein from 30 free-ranging long-tailed macaques. All samples were tested by the microscopic agglutination test. The LipL32 gene was used to detect pathogenic Leptospira in blood samples by conventional polymerase chain reaction. Results: Screening of the 30 wild macaques showed an overall Leptospira seroreactivity of 13.33%. Three of 30 macaques reacted against Leptospira serovar Shermani and one macaque was infected with Leptospira serovar Sejroe. None of the macaques presented clinical signs of leptospirosis. None of the blood samples showed the detection of the LipL32 gene. Conclusions: The results indicate that the long-tailed macaques at Kosumpee Forest Park may act as natural reservoirs for Leptospirosis. Further, the results provide evidence-based information indicating that several pathogenic Leptospira serovars are circulating in the wild macaques in the study area.

Microplastic contamination in food systems has emerged as a growing concern for food safety and public health. The presence of microplastics in raw milk may represent a potential exposure pathway for both animals and humans. This study investigated the occurrence and characteristics of microplastics in raw milk collected from smallholder dairy farms in Northeastern Thailand. Hand-milked raw milk and bulk tank milk samples were obtained from ten farms and analyzed for microplastic contamination. Suspected microplastic particles were identified and quantified using stereomicroscopy and characterized according to their shape, color, and size, while polymer composition was confirmed by Fourier Transform Infrared Spectroscopy. Microplastics were detected in both hand-milked and bulk tank milk samples, with fiber-shaped particles being the most frequently observed. The majority of detected particles were 0.05–0.15 mm in size and predominantly yellow in color. Polymer analysis revealed that Polydimethylsiloxane, followed by a semi-synthetic composite of Elastane and Rayon. These findings demonstrate that microplastics can be present in raw milk produced by smallholder dairy farms, highlighting the need for improved farm management and milk-handling practices to reduce contamination risks. From a One Health perspective, reducing plastic use and enhancing hygiene during milking and milk storage may help protect animal health, food safety, and consumer well-being.