The use of prestressed precast hollow core slabs has intensified as technological advances. However, the knowledge of the structural behavior when openings are inserted into this element is still limited, mainly due to the shear force. Therefore, the present study aims to analyze the structural behavior of the shear test of prestressed hollow core slabs with openings. In this paper, three types of hollow core slabs were tested: no openings, a central opening and side openings; using experimental and numerical methodology. The experimental test was carried out in the Federal University of São Carlos, and the numerical analysis used the software ABAQUS. All results were compared with three standard formulations (i.e., NBR, ACI and Eurocode), in order to verify its accuracy. In the end, the numerical results demonstrated that the developed model (CDP) presented results close to the experimental results. For the hollow core slab with a central opening, a rupture occurred in the web adjacent to the opening. On the other hand, for the hollow core slab with side openings, the rupture occurred at the edge of the web. Therefore, it was possible to conclude that the openings influence the main web tensions, being responsible for the diagonal model stress rupture. Finally, the Brazilian and Eurocode standard formulations proved to be good estimators of the resistant shear force.