In this paper, water-soluble 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized cellulose with a high degree of oxidation was prepared by a two-step process using bamboo dissolving pulp. The first step was to destroy the cellulose crystal I by NaOH/urea solution to obtain cellulose powder with decreased crystallinity. The second step was to oxidize the cellulose powder by TEMPO oxidation. The TEMPO-oxidized cellulose was analyzed by Fourier transform infrared spectroscopy (FTIR), conductimetry, X-ray diffraction (XRD), fiber analyzer, and transmission electron microscopy (TEM). FTIR showed that the hydroxymethyl groups in cellulose chains were converted into carboxyl groups. The degree of oxidation measured by conductimetry titration was as high as 91.0%. The TEMPO-oxidized cellulose was soluble in water for valuable polyelectrolytes and intermediates.

In the present study, the immobilization of free pectinase onto polystyrene resin beads via crosslinking with glutaraldehyde was investigated. The immobilized pectinase was characterized by Fourier transform infrared spectroscopy and confocal laser scanning microscopy. After optimizing the immobilization conditions, the optimum pH of immobilized pectinase shifted from 8.0 to 8.5 and the optimum temperature shifted from 45 to 60 °C, showing its improved stability to temperature and pH compared with the free pectinase. The Michaelis–Menten constant K_m value of free and immobilized pectinase was determined to be 1.95 and 5.36 mM, respectively. The storage stability of immobilized pectinase was demonstrated with 36.8% of the initial activity preserved after 30 days at 25 °C. The reusability of the immobilized pectinase activity was 54.6% of its initial activity after being recycled six times. Therefore, based on the findings mentioned above, it can be inferred that this simple immobilization technique for pectinase appears to be promising for industrial applications.