Antimicrobial resistance is an important health issue in human and veterinary medicine. The aim of this study was to monitor the antimicrobial resistance of three of the most important bacteria involved in porcine respiratory disease. A total of 465 isolates were tested during the 2017–2022 period for antimicrobial susceptibility for Actinobacillus pleuropneumoniae (n = 137), Streptococcus suis (n = 207), and Pasteurella multocida (n = 121) by disk diffusion method. The results were interpreted by CLSI breakpoints, where available. High rates of susceptibility (from 90 to >99%) were observed for cefquinome, ceftiofur, amoxicillin + clavulanic acid, amoxicillin, penicillin, ampicillin, florfenicol, enrofloxacin, marbofloxacin, and trimethoprim–sulfamethoxazole. A. pleuropneumoniae isolates showed high resistance to streptomycin (77%), gentamycin (45%), tilmicosin (39%) erythromycin (33%), oxytetracycline (19%), and tetracycline (18%). For S. suis, the highest rates of resistance were observed for streptomycin (98%), tetracycline (75%), oxytetracycline (72%), doxycycline (52%), and erythromycin (51%). P. multocida presented a high rate of resistance for streptomycin (63%), tilmicosin (29%), oxytetracycline (13%), and tetracycline (14%). Bacteria isolates maintained high susceptibility against antimicrobial agents usually used against the mainly respiratory tract pathogens of swine. Resistance for streptomycin, tetracycline, oxytetracycline, and tilmicosin was high for all the tested pathogens

Agonal thrombus is infrequently discussed in veterinary forensic pathology, being misdiagnosed as postmortem coagulation. The main purpose of the present study is to confirm that agonal thrombosis is an important tool in ruling out sudden death and to characterize it by gross, histological, and immunohistochemical approaches. The investigations have been conducted on 56 domestic carnivores. Fibrin was observed as rosette—like arrangements around platelet aggregates, loose network, wave—like pattern or short fibers and the additional tendency of lines of Zahn being noted inconsistently. All agonal thrombi had positive reactions for anti—CD61 for platelets, disposed in variable—sized clumps or in a linear pattern close to endothelial cells of endocardium. The same positive reaction has been noted to anti—fibrinogen and anti—fibrin antibodies. CD45, CD68 and von Willebrand factor had a very low to absent expression. Cardiac lesions were found in 22 cases (39.29%) suggesting predisposition to agonal thrombosis in animals with cardiovascular diseases. The results prove that agonal thrombus is a third category of blood coagulation that forms strictly during agonal death. The microscopical findings describe the agonal thrombus similar to the morphology of recent thrombus vera. Given the results, the agonal thrombus is a useful tool that confirms the agonal suffering prior to death.

The subject of the consumption of insects such as the house cricket (Acheta domesticus) and mealworm larvae (Tenebrio molitor) is an increasingly debated topic in the media considering the changes in food traditions influenced by inter-ethnic cultural exchanges as well as of the need to identify new nutritional sources with increased energy intake also related to legislative developments. The practical activity of the study was carried out in an authorized veterinary sanitary and food safety laboratory and aimed to study the transfer rate of lead from experimentally contaminated feed with this element at the value of 0.15 mg/kg in edible insects fed for 14 days with this feed. Quantitative determinations of lead in insects were made at the initial moment, later at 7 days and 14 days intervals, on 6 analytical lots for each type of insects. The method of lead determination was graphite furnace atomic absorption spectrometry. Analytical results demonstrated that the studied insects responded relatively uniformly to the degree of lead absorption during the first 7 days, namely an increase in lead levels of 41.2% in house crickets versus 40.25% in mealworm larvae.

Animal abuse and neglect represent important challenges in companion-animal practice, with veterinarians playing a central role in their identification and management. This study investigated how Romanian companion-animal veterinarians recognise and manage suspected cases of abuse and neglect, and explored the relationships between professional training, familiarity with animal welfare legislation, and clinical practices. A cross-sectional survey was conducted using an original online questionnaire completed by 137 veterinarians. Descriptive statistics and inferential analyses were applied. Although 73% of respondents reported encountering suspected abuse cases, 93.4% said they had never received formal training in abuse identification, and 78.1% worked in practices without specific protocols for managing such cases. Reporting rates were low, with 71.5% of veterinarians never having submitted an official report. Neglect was identified as the most frequently encountered form of abuse. Significant associations were observed between familiarity with animal welfare legislation, level of training, and awareness of neglect, and veterinarians who perceived themselves as less prepared were more likely to express a need for additional education. These findings highlight critical gaps in veterinary training and institutional support, emphasising the need for structured educational programmes, lifelong learning and continuing professional development, standardised clinical procedures, and closer collaboration with authorities to improve animal welfare outcomes.