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Prof. Quirico Migheli
Università degli Studi di Sassari — UNISS Department of Agriculture

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0 Plant Pathology
0 mycotoxin
0 Aspergillus
0 Fusarium
0 pathogenic fungi

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Fusarium
mycotoxin
Aspergillus
pathogenic fungi
Plant Pathology

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Short Biography

Quirico Migheli is professor of Plant pathology at the Department of Agricultural Sciences, University of Sassari, Italy. Since 2016 is Editor-in-Chief of the T&F journal Biocontrol Science & Technology. Currently chair of the BSc degree in Security and International Cooperation at the University of Sassari. His research interests include: food safety and food security issues; molecular and physiological plant-pathogen interactions; biological control of mycotoxin-producing fungi; risk assessment of biocontrol agents. Bibliometrics: H-index: 31 (Scopus) - 38 (Scholar); i10-Index: 81 (Scholar).

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Research article
Published: 14 May 2021 in Mycologia
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Several Fusarium species cause disease on human hosts, including commonly fatal infections in immunocompromised individuals. Recently, cases of hospitalized patients affected by fusaria were reported in the Tyrrhenian Island of Sardinia, Italy. To precisely characterize the Fusarium species and haplotypes present in hospitals of the region, a multilocus DNA sequence typing (MLST) approach was applied. Water distribution systems in four departments belonging to four Sardinian hospitals were sampled. Fusarium species and sequence types (STs) were identified using MLST based on sequences of the elongation factor 1-alpha (EF-1α) gene, the nuclear ribosomal DNA intergenic spacer region (IGS rDNA), and/or a portion of the second-largest subunit of RNA polymerase (RPB2) gene. The majority of isolates obtained from Sardinian hospitals (90.7%) were identified as representatives of the Fusarium oxysporum species complex (FOSC), followed by those of the F. solani species complex (FSSC) (8.2%), and F. dimerum (1.1% of all isolates). Ten STs were found among the FOSC and FSSC, with more than 60% of the isolates identified as either FOSC ST 33 or FSSC 1 (F. petroliphilum). More than half of the FOSC isolates obtained from the water systems in all four hospitals belonged to the worldwide distributed clonal lineage ST 33. This haplotype is the most prevalent among the FOSC in different countries, being responsible for the vast majority of cases of human fusariosis.

ACS Style

Virgilio Balmas; Francesca Fancellu; Silvana Sanna; Barbara Scherm; Quirico Migheli; Ismael Malbrán. Water distribution systems in Sardinian hospitals host invasive clonal lineages of the Fusarium oxysporum and Fusarium solani species complexes. Mycologia 2021, 1 -9.

AMA Style

Virgilio Balmas, Francesca Fancellu, Silvana Sanna, Barbara Scherm, Quirico Migheli, Ismael Malbrán. Water distribution systems in Sardinian hospitals host invasive clonal lineages of the Fusarium oxysporum and Fusarium solani species complexes. Mycologia. 2021; ():1-9.

Chicago/Turabian Style

Virgilio Balmas; Francesca Fancellu; Silvana Sanna; Barbara Scherm; Quirico Migheli; Ismael Malbrán. 2021. "Water distribution systems in Sardinian hospitals host invasive clonal lineages of the Fusarium oxysporum and Fusarium solani species complexes." Mycologia , no. : 1-9.

Journal article
Published: 22 February 2021 in Environmental Technology & Innovation
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Mycotoxins, the metabolites of toxigenic fungi are important contaminants of food and agriculture industry throughout the world. Among the different strategies to minimize the mycotoxins synthesis and decontamination of food; yeasts and their derivatives are used as efficient biological control agents. In this study, cell walls (CW) of six yeast strains; two commercial baking {Saccharomyces cerevisiae (Sc 1 and Sc2)}, two low-fermenting (Candida intermedia and Lachancea thermotolerans), and two non-fermenting (Cyberlindnera jadinii and Candida friedrichii) were explored to inhibit A. flavus growth, aflatoxin (AF) synthesis and remove mycotoxins from contaminated buffers (at pH 3, 5 and 7) and milk. The volatiles of non-fermenting yeasts showed a significant inhibitory effect on of A. flavus growth (up to 79%) and aflatoxin synthesis potential. The highest mycotoxins binding activities of Sc strains were noted against ochratoxin A (92%), AFB2 (66%), AFG2 (59%) and AFB1 (31%). The highest and lowest binding activities occurred at pH 7 and pH 3, respectively. On the other hand, all yeast CWs significantly (44%–54%) reduced aflatoxin M1 (AFM1) from contaminated milk. These results show clearly that yeasts (particularly Sc) can be used as efficient biocontrol and decontamination agents in food industry or the management of mycotoxin contamination.

ACS Style

Zahoor Ul Hassan; Roda Al Thani; Fathy A. Atia; Mohammed Alsafran; Quirico Migheli; Samir Jaoua. Application of yeasts and yeast derivatives for the biological control of toxigenic fungi and their toxic metabolites. Environmental Technology & Innovation 2021, 22, 101447 .

AMA Style

Zahoor Ul Hassan, Roda Al Thani, Fathy A. Atia, Mohammed Alsafran, Quirico Migheli, Samir Jaoua. Application of yeasts and yeast derivatives for the biological control of toxigenic fungi and their toxic metabolites. Environmental Technology & Innovation. 2021; 22 ():101447.

Chicago/Turabian Style

Zahoor Ul Hassan; Roda Al Thani; Fathy A. Atia; Mohammed Alsafran; Quirico Migheli; Samir Jaoua. 2021. "Application of yeasts and yeast derivatives for the biological control of toxigenic fungi and their toxic metabolites." Environmental Technology & Innovation 22, no. : 101447.

Journal article
Published: 27 January 2021 in Molecules
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Onychomycosis is a common nail infection mainly caused by species belonging to the F. oxysporum, F. solani, and F. fujikuroi species complexes. The aim of this study was to evaluate the in vitro susceptibility of six representative strains of clinically relevant Fusarium spp. toward a set of natural-occurring hydroxycinnamic acids and their derivatives with the purpose to develop naturally occurring products in order to cope with emerging resistance phenomena. By introducing a prenylated chain at one of the hydroxy groups of trans-cinnamic acids 1–3, ten prenylated derivatives (coded 4–13) were preliminarily investigated in solid Fusarium minimal medium (FMM). Minimal inhibitory concentration (MIC) and lethal dose 50 (LD50) values were then determined in liquid FMM for the most active selected antifungal p-coumaric acid 3,3′-dimethyl allyl ester 13, in comparison with the conventional fungicides terbinafine (TRB) and amphotericin B (AmB), through the quantification of the fungal growth. Significant growth inhibition was observed for prenylated derivatives 4–13, evidencing ester 13 as the most active. This compound presented MIC and LD50 values (62–250 µM and 7.8–125 µM, respectively) comparable to those determined for TRB and AmB in the majority of the tested pathogenic strains. The position and size of the prenylated chain and the presence of a free phenol OH group appear crucial for the antifungal activity. This work represents the first report on the activity of prenylated cinnamic esters and ethers against clinical Fusarium spp. and opens new avenues in the development of alternative antifungal compounds based on a drug repositioning strategy.

ACS Style

Safa Oufensou; Stefano Casalini; Virgilio Balmas; Paola Carta; Wiem Chtioui; Maria Dettori; Davide Fabbri; Quirico Migheli; Giovanna Delogu. Prenylated Trans-Cinnamic Esters and Ethers Against Clinical Fusarium spp.: Repositioning of Natural Compounds in Antimicrobial Discovery. Molecules 2021, 26, 658 .

AMA Style

Safa Oufensou, Stefano Casalini, Virgilio Balmas, Paola Carta, Wiem Chtioui, Maria Dettori, Davide Fabbri, Quirico Migheli, Giovanna Delogu. Prenylated Trans-Cinnamic Esters and Ethers Against Clinical Fusarium spp.: Repositioning of Natural Compounds in Antimicrobial Discovery. Molecules. 2021; 26 (3):658.

Chicago/Turabian Style

Safa Oufensou; Stefano Casalini; Virgilio Balmas; Paola Carta; Wiem Chtioui; Maria Dettori; Davide Fabbri; Quirico Migheli; Giovanna Delogu. 2021. "Prenylated Trans-Cinnamic Esters and Ethers Against Clinical Fusarium spp.: Repositioning of Natural Compounds in Antimicrobial Discovery." Molecules 26, no. 3: 658.

Research article
Published: 17 January 2021 in Biocontrol Science and Technology
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ACS Style

Quirico Migheli. Editorial. Biocontrol Science and Technology 2021, 31, 602 -603.

AMA Style

Quirico Migheli. Editorial. Biocontrol Science and Technology. 2021; 31 (6):602-603.

Chicago/Turabian Style

Quirico Migheli. 2021. "Editorial." Biocontrol Science and Technology 31, no. 6: 602-603.

Journal article
Published: 24 December 2020 in Fermentation
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Red yeasts grow on food wastes, show antagonistic activity against food-spoilage microorganisms, produce food supplements, and may be utilized as feed-supplements themselves to positively modulate the quali-quantitative composition of intestinal microbiota. Therefore, they show a variety of possible biotechnological applications in agri-food-related processes. Here, to further explore the biotechnological potential of red yeasts, eleven strains ascribed to different species of the genera Rhodotorula and Sporobolomyces, differing for biomass and carotenoids production, were characterized in vitro for biofilm formation, invasive growth, and growth at the temperature range of 20–40 °C and in vivo for their antagonistic activity against the fungal pathogen and patulin producer Penicillium expansum. Most of them formed cellular MAT and showed invasive growth as well as adhesion to plastic materials. Four strains determined a significant reduction of fruit decay caused by P. expansum on apple fruit while the remaining seven showed different degrees of biocontrol activity. Finally, none of them grew at body temperature (>37 °C). Statistical analyses of both qualitative and quantitative phenotypic data, including biomass and carotenoids production, gathered further information on the most interesting strains for the biotechnological exploitation of red yeasts in agri-food-related process.

ACS Style

Giacomo Zara; Maria Grazia Farbo; Chiara Multineddu; Quirico Migheli; Marilena Budroni; Severino Zara; Ilaria Mannazzu. Exploring the Biodiversity of Red Yeasts for In Vitro and In Vivo Phenotypes Relevant to Agri-Food-Related Processes. Fermentation 2020, 7, 2 .

AMA Style

Giacomo Zara, Maria Grazia Farbo, Chiara Multineddu, Quirico Migheli, Marilena Budroni, Severino Zara, Ilaria Mannazzu. Exploring the Biodiversity of Red Yeasts for In Vitro and In Vivo Phenotypes Relevant to Agri-Food-Related Processes. Fermentation. 2020; 7 (1):2.

Chicago/Turabian Style

Giacomo Zara; Maria Grazia Farbo; Chiara Multineddu; Quirico Migheli; Marilena Budroni; Severino Zara; Ilaria Mannazzu. 2020. "Exploring the Biodiversity of Red Yeasts for In Vitro and In Vivo Phenotypes Relevant to Agri-Food-Related Processes." Fermentation 7, no. 1: 2.

Research article
Published: 17 November 2020 in Phytopathology®
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Scientific communication is facilitated by a data-driven, scientifically sound taxonomy that considers the end-user's needs and established successful practice. Previously (Geiser et al. 2013; Phytopathology 103:400-408. 2013), the Fusarium community voiced near unanimous support for a concept of Fusarium that represented a clade comprising all agriculturally and clinically important Fusarium species, including the F. solani Species Complex (FSSC). Subsequently, this concept was challenged by one research group (Lombard et al. 2015 Studies in Mycology 80: 189-245) who proposed dividing Fusarium into seven genera, including the FSSC as the genus Neocosmospora, with subsequent justification based on claims that the Geiser et al. (2013) concept of Fusarium is polyphyletic (Sandoval-Denis et al. 2018; Persoonia 41:109-129). Here we test this claim, and provide a phylogeny based on exonic nucleotide sequences of 19 orthologous protein-coding genes that strongly support the monophyly of Fusarium including the FSSC. We reassert the practical and scientific argument in support of a Fusarium that includes the FSSC and several other basal lineages, consistent with the longstanding use of this name among plant pathologists, medical mycologists, quarantine officials, regulatory agencies, students and researchers with a stake in its taxonomy. In recognition of this monophyly, 40 species recently described as Neocosmospora were recombined in Fusarium, and nine others were renamed Fusarium. Here the global Fusarium community voices strong support for the inclusion of the FSSC in Fusarium, as it remains the best scientific, nomenclatural and practical taxonomic option available.

ACS Style

David M Geiser; Abdullah Al-Hatmi; Takayuki Aoki; Tsutomu Arie; Virgilio Balmas; Irene Barnes; Gary C Bergstrom; M.K. K. Bhattacharyya; Cheryl L. Blomquist; Robert Bowden; Balázs Brankovics; Daren W. Brown; Lester William Burgess; Kathryn Bushley; Mark Busman; José F. Cano-Lira; Joseph D. Carrillo; Hao-Xun Chang; Chi-Yu Chen; Wanquan Chen; Martin I. Chilvers; Sofia Noemi Chulze; Jeffrey J. Coleman; Christina A. Cuomo; Z. Wilhelm De Beer; G. Sybren De Hoog; Johanna Del Castillo-Múnera; Emerson Del Ponte; Javier Diéguez-Uribeondo; Antonio Di Pietro; Vérnonique Edel-Hermann; Wade H Elmer; Lynn Epstein; Akif Eskalen; Maria Carmela Esposto; Kathryne L. Everts; Sylvia P. Fernández-Pavía; Gilvan Ferreira Da Silva; Nora A Foroud; Gerda Fourie; Rasmus J.N. Frandsen; Stanley Freeman; Michael Freitag; Omer Frenkel; Kevin K Fuller; Tatiana Gagkaeva; Donald Max Gardiner; Anthony E. Glenn; Scott Gold; Tom Gordon; Nancy F. Gregory; Marieka Gryzenhout; Josep Guarro; Beth Gugino; Santiago Gutiérrez; Kim Hammond-Kosack; Linda J. Harris; Mónika Homa; Cheng-Fang Hong; László Hornok; Jenn-Wen Huang; Macit Ilkit; Adriaana Jacobs; Karin Jacobs; Cong Jiang; Maria Del Mar Jimenez-Gasco; Seogchan Kang; Matthew T Kasson; Kemal Kazan; John Carlyle Kennell; Hyeseon Kim; Harold Corby Kistler; Gretchen A. Kuldau; Tomasz Kulik; Oliver Kurzai; Imane Laraba; Matthew H. Laurence; Theresa Yun Lee; Yin-Won Lee; Yong-Hwan Lee; John F. Leslie; Edward C.Y. Liew; Lily W. Lofton; Antonio Logrieco; Manuel Sánchez López-Berges; Alicia Graciela Luque; Erik Lysøe; Li-Jun Ma; Robert E Marra; Frank N. Martin; Sara Ruth May; Susan McCormick; Chyanna T McGee; Jacques F. Meis; Quirico Migheli; Nik Mohd Izham Mohamed Nor; Michel Monod; Antonio Moretti; Diane Mostert; Giuseppina. Mulé; Françoise Munaut; Gary P Munkvold; Paul Nicholson; Marcio Nucci; Kerry O'Donnell; Matias Pasquali; Ludwig H. Pfenning; Anna Prigitano; Robert Proctor; Stéphane Ranque; Stephen Rehner; Martijn Rep; Gerardo Rodríguez-Alvarado; Lindy J Rose; Mitchell George Roth; Carmen Ruiz-Roldán; Amgad A Saleh; Baharuddin Salleh; Hyunkyu Sang; Mercedes Scandiani; Jonathan Scauflaire; David Schmale; Dylan Pg Short; Adnan Šišić; Jason Smith; Christopher W. Smyth; Hokyoung Son; Ellie Spahr; Jason E Stajich; Emma Steenkamp; Christian Steinberg; Rajagopal Subramaniam; Haruhisa Suga; Brett Anthony Summerell; Antonella Susca; Cassandra Lynn Swett; Christopher Toomajian; Terry Jarianna Torres-Cruz; Anna Maria Tortorano; Martin Urban; Lisa J. Vaillancourt; Gary E Vallad; Theo Van Der Lee; Dan Vanderpool; Anne D. Van Diepeningen; Martha Vaughan; Eduard Venter; Marcele Vermeulen; Paul E. Verweij; Altus Viljoen; Cees Waalwijk; Emma C. Wallace; Grit Walther; Jie Wang; Todd Ward; Brian Wickes; Nathan P. Wiederhold; Michael J. Wingfield; Ana K.M. Wood; Jin-Rong Xu; X. B. Yang; Tapani Yli-Matilla; Sung-Hwan Yun; Latiffah Zakaria; Hao Zhang; Ning Zhang; Sean Zhang; Xue Zhang. Phylogenomic analysis of a 55.1 kb 19-gene dataset resolves a monophyletic Fusarium that includes the Fusarium solani Species Complex. Phytopathology® 2020, 1 .

AMA Style

David M Geiser, Abdullah Al-Hatmi, Takayuki Aoki, Tsutomu Arie, Virgilio Balmas, Irene Barnes, Gary C Bergstrom, M.K. K. Bhattacharyya, Cheryl L. Blomquist, Robert Bowden, Balázs Brankovics, Daren W. Brown, Lester William Burgess, Kathryn Bushley, Mark Busman, José F. Cano-Lira, Joseph D. Carrillo, Hao-Xun Chang, Chi-Yu Chen, Wanquan Chen, Martin I. Chilvers, Sofia Noemi Chulze, Jeffrey J. Coleman, Christina A. Cuomo, Z. Wilhelm De Beer, G. Sybren De Hoog, Johanna Del Castillo-Múnera, Emerson Del Ponte, Javier Diéguez-Uribeondo, Antonio Di Pietro, Vérnonique Edel-Hermann, Wade H Elmer, Lynn Epstein, Akif Eskalen, Maria Carmela Esposto, Kathryne L. Everts, Sylvia P. Fernández-Pavía, Gilvan Ferreira Da Silva, Nora A Foroud, Gerda Fourie, Rasmus J.N. Frandsen, Stanley Freeman, Michael Freitag, Omer Frenkel, Kevin K Fuller, Tatiana Gagkaeva, Donald Max Gardiner, Anthony E. Glenn, Scott Gold, Tom Gordon, Nancy F. Gregory, Marieka Gryzenhout, Josep Guarro, Beth Gugino, Santiago Gutiérrez, Kim Hammond-Kosack, Linda J. Harris, Mónika Homa, Cheng-Fang Hong, László Hornok, Jenn-Wen Huang, Macit Ilkit, Adriaana Jacobs, Karin Jacobs, Cong Jiang, Maria Del Mar Jimenez-Gasco, Seogchan Kang, Matthew T Kasson, Kemal Kazan, John Carlyle Kennell, Hyeseon Kim, Harold Corby Kistler, Gretchen A. Kuldau, Tomasz Kulik, Oliver Kurzai, Imane Laraba, Matthew H. Laurence, Theresa Yun Lee, Yin-Won Lee, Yong-Hwan Lee, John F. Leslie, Edward C.Y. Liew, Lily W. Lofton, Antonio Logrieco, Manuel Sánchez López-Berges, Alicia Graciela Luque, Erik Lysøe, Li-Jun Ma, Robert E Marra, Frank N. Martin, Sara Ruth May, Susan McCormick, Chyanna T McGee, Jacques F. Meis, Quirico Migheli, Nik Mohd Izham Mohamed Nor, Michel Monod, Antonio Moretti, Diane Mostert, Giuseppina. Mulé, Françoise Munaut, Gary P Munkvold, Paul Nicholson, Marcio Nucci, Kerry O'Donnell, Matias Pasquali, Ludwig H. Pfenning, Anna Prigitano, Robert Proctor, Stéphane Ranque, Stephen Rehner, Martijn Rep, Gerardo Rodríguez-Alvarado, Lindy J Rose, Mitchell George Roth, Carmen Ruiz-Roldán, Amgad A Saleh, Baharuddin Salleh, Hyunkyu Sang, Mercedes Scandiani, Jonathan Scauflaire, David Schmale, Dylan Pg Short, Adnan Šišić, Jason Smith, Christopher W. Smyth, Hokyoung Son, Ellie Spahr, Jason E Stajich, Emma Steenkamp, Christian Steinberg, Rajagopal Subramaniam, Haruhisa Suga, Brett Anthony Summerell, Antonella Susca, Cassandra Lynn Swett, Christopher Toomajian, Terry Jarianna Torres-Cruz, Anna Maria Tortorano, Martin Urban, Lisa J. Vaillancourt, Gary E Vallad, Theo Van Der Lee, Dan Vanderpool, Anne D. Van Diepeningen, Martha Vaughan, Eduard Venter, Marcele Vermeulen, Paul E. Verweij, Altus Viljoen, Cees Waalwijk, Emma C. Wallace, Grit Walther, Jie Wang, Todd Ward, Brian Wickes, Nathan P. Wiederhold, Michael J. Wingfield, Ana K.M. Wood, Jin-Rong Xu, X. B. Yang, Tapani Yli-Matilla, Sung-Hwan Yun, Latiffah Zakaria, Hao Zhang, Ning Zhang, Sean Zhang, Xue Zhang. Phylogenomic analysis of a 55.1 kb 19-gene dataset resolves a monophyletic Fusarium that includes the Fusarium solani Species Complex. Phytopathology®. 2020; ():1.

Chicago/Turabian Style

David M Geiser; Abdullah Al-Hatmi; Takayuki Aoki; Tsutomu Arie; Virgilio Balmas; Irene Barnes; Gary C Bergstrom; M.K. K. Bhattacharyya; Cheryl L. Blomquist; Robert Bowden; Balázs Brankovics; Daren W. Brown; Lester William Burgess; Kathryn Bushley; Mark Busman; José F. Cano-Lira; Joseph D. Carrillo; Hao-Xun Chang; Chi-Yu Chen; Wanquan Chen; Martin I. Chilvers; Sofia Noemi Chulze; Jeffrey J. Coleman; Christina A. Cuomo; Z. Wilhelm De Beer; G. Sybren De Hoog; Johanna Del Castillo-Múnera; Emerson Del Ponte; Javier Diéguez-Uribeondo; Antonio Di Pietro; Vérnonique Edel-Hermann; Wade H Elmer; Lynn Epstein; Akif Eskalen; Maria Carmela Esposto; Kathryne L. Everts; Sylvia P. Fernández-Pavía; Gilvan Ferreira Da Silva; Nora A Foroud; Gerda Fourie; Rasmus J.N. Frandsen; Stanley Freeman; Michael Freitag; Omer Frenkel; Kevin K Fuller; Tatiana Gagkaeva; Donald Max Gardiner; Anthony E. Glenn; Scott Gold; Tom Gordon; Nancy F. Gregory; Marieka Gryzenhout; Josep Guarro; Beth Gugino; Santiago Gutiérrez; Kim Hammond-Kosack; Linda J. Harris; Mónika Homa; Cheng-Fang Hong; László Hornok; Jenn-Wen Huang; Macit Ilkit; Adriaana Jacobs; Karin Jacobs; Cong Jiang; Maria Del Mar Jimenez-Gasco; Seogchan Kang; Matthew T Kasson; Kemal Kazan; John Carlyle Kennell; Hyeseon Kim; Harold Corby Kistler; Gretchen A. Kuldau; Tomasz Kulik; Oliver Kurzai; Imane Laraba; Matthew H. Laurence; Theresa Yun Lee; Yin-Won Lee; Yong-Hwan Lee; John F. Leslie; Edward C.Y. Liew; Lily W. Lofton; Antonio Logrieco; Manuel Sánchez López-Berges; Alicia Graciela Luque; Erik Lysøe; Li-Jun Ma; Robert E Marra; Frank N. Martin; Sara Ruth May; Susan McCormick; Chyanna T McGee; Jacques F. Meis; Quirico Migheli; Nik Mohd Izham Mohamed Nor; Michel Monod; Antonio Moretti; Diane Mostert; Giuseppina. Mulé; Françoise Munaut; Gary P Munkvold; Paul Nicholson; Marcio Nucci; Kerry O'Donnell; Matias Pasquali; Ludwig H. Pfenning; Anna Prigitano; Robert Proctor; Stéphane Ranque; Stephen Rehner; Martijn Rep; Gerardo Rodríguez-Alvarado; Lindy J Rose; Mitchell George Roth; Carmen Ruiz-Roldán; Amgad A Saleh; Baharuddin Salleh; Hyunkyu Sang; Mercedes Scandiani; Jonathan Scauflaire; David Schmale; Dylan Pg Short; Adnan Šišić; Jason Smith; Christopher W. Smyth; Hokyoung Son; Ellie Spahr; Jason E Stajich; Emma Steenkamp; Christian Steinberg; Rajagopal Subramaniam; Haruhisa Suga; Brett Anthony Summerell; Antonella Susca; Cassandra Lynn Swett; Christopher Toomajian; Terry Jarianna Torres-Cruz; Anna Maria Tortorano; Martin Urban; Lisa J. Vaillancourt; Gary E Vallad; Theo Van Der Lee; Dan Vanderpool; Anne D. Van Diepeningen; Martha Vaughan; Eduard Venter; Marcele Vermeulen; Paul E. Verweij; Altus Viljoen; Cees Waalwijk; Emma C. Wallace; Grit Walther; Jie Wang; Todd Ward; Brian Wickes; Nathan P. Wiederhold; Michael J. Wingfield; Ana K.M. Wood; Jin-Rong Xu; X. B. Yang; Tapani Yli-Matilla; Sung-Hwan Yun; Latiffah Zakaria; Hao Zhang; Ning Zhang; Sean Zhang; Xue Zhang. 2020. "Phylogenomic analysis of a 55.1 kb 19-gene dataset resolves a monophyletic Fusarium that includes the Fusarium solani Species Complex." Phytopathology® , no. : 1.

Review article
Published: 07 February 2020 in Frontiers in Microbiology
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The use of synthetic fungicides represents the most common strategy to control plant pathogens. Excessive and/or long-term distribution of chemicals is responsible for increased levels of environmental pollution, as well as adverse health consequence to humans and animals. These issues are deeply influencing public perception, as reflected by the increasing demand for safer and eco-friendly agricultural commodities and their by-products. A steadily increasing number of research efforts is now devoted to explore the use of safer and innovative approaches to control plant pathogens. The use of microorganisms as biological control agents (BCAs) represents one of the most durable and promising strategies. Among the panoply of microbial mechanisms exerted by BCAs, the production of volatile organic compounds (VOCs) represents an intriguing issue, mostly exploitable in circumstances where a direct contact between the pathogen and its antagonist is not practicable. VOCs are potentially produced by all living microorganisms, and may be active in the biocontrol of phytopathogenic oomycetes, fungi, and bacteria by means of antimicrobial activity and/or other cross-talk interactions. Their biological effects, the reduced residuals in the environment and on agricultural commodities, and the ease of application in different agricultural systems make the use of VOCs a promising and sustainable approach to replace synthetic fungicides in the control of plant pathogens. In this review, we focus on VOCs produced by bacteria and fungi and on their role in the cross-talk existing between the plant pathogens and their host. Biologic systemic effect of the microbial volatile blends on both pathogen and host plant cells is also briefly reviewed.

ACS Style

Bruno Tilocca; Aocheng Cao; Quirico Migheli. Scent of a Killer: Microbial Volatilome and Its Role in the Biological Control of Plant Pathogens. Frontiers in Microbiology 2020, 11, 41 .

AMA Style

Bruno Tilocca, Aocheng Cao, Quirico Migheli. Scent of a Killer: Microbial Volatilome and Its Role in the Biological Control of Plant Pathogens. Frontiers in Microbiology. 2020; 11 ():41.

Chicago/Turabian Style

Bruno Tilocca; Aocheng Cao; Quirico Migheli. 2020. "Scent of a Killer: Microbial Volatilome and Its Role in the Biological Control of Plant Pathogens." Frontiers in Microbiology 11, no. : 41.

Journal article
Published: 02 December 2019 in Toxins
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Mycotoxins are secondary metabolites produced by certain filamentous fungi, causing human and animal health issues upon the ingestion of contaminated food and feed. Among the safest approaches to the control of mycotoxigenic fungi and mycotoxin detoxification is the application of microbial biocontrol agents. Burkholderia cepacia is known for producing metabolites active against a broad number of pathogenic fungi. In this study, the antifungal potential of a Qatari strain of Burkholderia cepacia (QBC03) was explored. QBC03 exhibited antifungal activity against a wide range of mycotoxigenic, as well as phytopathogenic, fungal genera and species. The QBC03 culture supernatant significantly inhibited the growth of Aspergillus carbonarius, Fusarium culmorum and Penicillium verrucosum in PDA medium, as well as A. carbonarius and P. verrucosum biomass in PDB medium. The QBC03 culture supernatant was found to dramatically reduce the synthesis of ochratoxin A (OTA) by A. carbonarius, in addition to inducing mycelia malformation. The antifungal activity of QBC03’s culture extract was retained following thermal treatment at 100 °C for 30 min. The findings of the present study advocate that QBC03 is a suitable biocontrol agent against toxigenic fungi, due to the inhibitory activity of its thermostable metabolites.

ACS Style

Randa Zeidan; Zahoor Ul-Hassan; Roda Al-Thani; Quirico Migheli; Samir Jaoua. In-Vitro Application of a Qatari Burkholderia cepacia strain (QBC03) in the Biocontrol of Mycotoxigenic Fungi and in the Reduction of Ochratoxin A biosynthesis by Aspergillus carbonarius. Toxins 2019, 11, 700 .

AMA Style

Randa Zeidan, Zahoor Ul-Hassan, Roda Al-Thani, Quirico Migheli, Samir Jaoua. In-Vitro Application of a Qatari Burkholderia cepacia strain (QBC03) in the Biocontrol of Mycotoxigenic Fungi and in the Reduction of Ochratoxin A biosynthesis by Aspergillus carbonarius. Toxins. 2019; 11 (12):700.

Chicago/Turabian Style

Randa Zeidan; Zahoor Ul-Hassan; Roda Al-Thani; Quirico Migheli; Samir Jaoua. 2019. "In-Vitro Application of a Qatari Burkholderia cepacia strain (QBC03) in the Biocontrol of Mycotoxigenic Fungi and in the Reduction of Ochratoxin A biosynthesis by Aspergillus carbonarius." Toxins 11, no. 12: 700.

Review
Published: 01 October 2019 in World Journal of Microbiology and Biotechnology
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Yeasts occur in all environments and have been described as potent antagonists of various plant pathogens. Due to their antagonistic ability, undemanding cultivation requirements, and limited biosafety concerns, many of these unicellular fungi have been considered for biocontrol applications. Here, we review the fundamental research on the mechanisms (e.g., competition, enzyme secretion, toxin production, volatiles, mycoparasitism, induction of resistance) by which biocontrol yeasts exert their activity as plant protection agents. In a second part, we focus on five yeast species (Candida oleophila, Aureobasidium pullulans, Metschnikowia fructicola, Cryptococcus albidus, Saccharomyces cerevisiae) that are or have been registered for the application as biocontrol products. These examples demonstrate the potential of yeasts for commercial biocontrol usage, but this review also highlights the scarcity of fundamental studies on yeast biocontrol mechanisms and of registered yeast-based biocontrol products. Yeast biocontrol mechanisms thus represent a largely unexplored field of research and plentiful opportunities for the development of commercial, yeast-based applications for plant protection exist.

ACS Style

Florian M. Freimoser; Maria Paula Rueda-Mejia; Bruno Tilocca; Quirico Migheli. Biocontrol yeasts: mechanisms and applications. World Journal of Microbiology and Biotechnology 2019, 35, 1 -19.

AMA Style

Florian M. Freimoser, Maria Paula Rueda-Mejia, Bruno Tilocca, Quirico Migheli. Biocontrol yeasts: mechanisms and applications. World Journal of Microbiology and Biotechnology. 2019; 35 (10):1-19.

Chicago/Turabian Style

Florian M. Freimoser; Maria Paula Rueda-Mejia; Bruno Tilocca; Quirico Migheli. 2019. "Biocontrol yeasts: mechanisms and applications." World Journal of Microbiology and Biotechnology 35, no. 10: 1-19.

Disease note
Published: 29 July 2019 in Journal of Plant Pathology
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Bruno Tilocca; Safa Oufensou; Eleonora Atzeri; Giacomo Loddo; Gianluigi Murgia; Stefania Oggiano; Virgilio Balmas; Quirico Migheli. First report of a member of the Fusarium oxysporum species complex on Euphorbia pulcherrima Willd. ex Klotzsch in Italy. Journal of Plant Pathology 2019, 102, 233 -233.

AMA Style

Bruno Tilocca, Safa Oufensou, Eleonora Atzeri, Giacomo Loddo, Gianluigi Murgia, Stefania Oggiano, Virgilio Balmas, Quirico Migheli. First report of a member of the Fusarium oxysporum species complex on Euphorbia pulcherrima Willd. ex Klotzsch in Italy. Journal of Plant Pathology. 2019; 102 (1):233-233.

Chicago/Turabian Style

Bruno Tilocca; Safa Oufensou; Eleonora Atzeri; Giacomo Loddo; Gianluigi Murgia; Stefania Oggiano; Virgilio Balmas; Quirico Migheli. 2019. "First report of a member of the Fusarium oxysporum species complex on Euphorbia pulcherrima Willd. ex Klotzsch in Italy." Journal of Plant Pathology 102, no. 1: 233-233.

Journal article
Published: 10 July 2019 in International Journal of Food Microbiology
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The use of yeast-derived volatile organic compounds (VOCs) represents a promising strategy for the biological control of various plant pathogens, including mycotoxin-producing fungi. Previous studies demonstrated the efficacy of the low-fermenting yeast Candida intermedia isolate 253 in reducing growth, sporulation, and ochratoxin A biosynthesis by Aspergillus carbonarius MPVA566. This study aimed to investigate whether the inhibitory effect of the yeast volatilome is solely attributable to 2-phenylethanol, its major component, or if a synergistic effect of all volatilome components is required to achieve an effective control of the fungal growth and metabolism. Microbiological methods, HPLC measurements and a UPLC-MS/MS approach were used to investigate the metabolic profile of A. carbonarius MPVA566 at different growing conditions: standard incubation (control), exposed to C. intermedia 253 volatilome, and incubation in the presence of 2-phenylethanol. Both yeast volatilome and 2-phenylethanol succeeded in the macroscopic inhibition of the radial mycelial growth, along with a significant reduction of ochratoxin A production. Functional classification of the fungal proteome identified in the diverse growing conditions revealed a different impact of both yeast VOCs and 2-phenylethanol exposure on the fungal proteome. Yeast VOCs target an array of metabolic routes of fungal system biology, including a marked reduction in protein biosynthesis, proliferative activity, mitochondrial metabolism, and particularly in detoxification of toxic substances. Exposure to 2-phenylethanol only partially mimicked the metabolic effects observed by the whole yeast volatilome, with protein biosynthesis and proliferative activity being reduced when compared with the control samples, but still far from the VOCs-exposed condition. This study represents the first investigation on the effects of yeast-derived volatilome and 2-phenylethanol on the metabolism of a mycotoxigenic fungus by means of proteomics analysis. 2-Phenylethanol (PubChem CID: 6054); ochratoxin-A (PubChem CID: 442530); sodium dodecyl sulfate (PubChem CID: 3423265); dithiothreitol (PubChem CID: 446094); phenylmethylsulfonyl fluoride (PubChem CID: 4784); iodoacetamide (PubChem CID: 3727); ammonium bicarbonate (PubChem CID: 14013); acetic acid (PubChem CID: 176); and acetonitrile (PubChem CID: 6342).

ACS Style

Bruno Tilocca; Virgilio Balmas; Zahoor Ul Hassan; Samir Jaoua; Quirico Migheli. A proteomic investigation of Aspergillus carbonarius exposed to yeast volatilome or to its major component 2-phenylethanol reveals major shifts in fungal metabolism. International Journal of Food Microbiology 2019, 306, 108265 .

AMA Style

Bruno Tilocca, Virgilio Balmas, Zahoor Ul Hassan, Samir Jaoua, Quirico Migheli. A proteomic investigation of Aspergillus carbonarius exposed to yeast volatilome or to its major component 2-phenylethanol reveals major shifts in fungal metabolism. International Journal of Food Microbiology. 2019; 306 ():108265.

Chicago/Turabian Style

Bruno Tilocca; Virgilio Balmas; Zahoor Ul Hassan; Samir Jaoua; Quirico Migheli. 2019. "A proteomic investigation of Aspergillus carbonarius exposed to yeast volatilome or to its major component 2-phenylethanol reveals major shifts in fungal metabolism." International Journal of Food Microbiology 306, no. : 108265.

Journal article
Published: 28 May 2019 in Sensors
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A preliminary assessment of properties of the commercial product Chemiplus 2DS HB (BI-QEM Specialties SpA) is proposed. Cyclic voltammetry of this oligomer containing sulfate/sulfone groups shows a single oxidative peak at +0.866 V vs. Ag/AgCl, and its passivating process on Pt electrode suggests the formation of a non-conductive layer. Electrode modification was achieved by exploiting the constant potential amperometry setting potential at +0.900 V vs. Ag/AgCl. A substantial change in the oxidative currents from electroactive species H2O2 and ascorbic acid (AA) were observed on Pt/Chemiplus 2DS HB sensors compared to unmodified Pt. Furthermore, the influence of different storage conditions on modified sensors was examined. A storage solution containing AA concentration from 0.1 until 10 mM maintained effective AA rejection of Pt/Chemiplus 2DS HB after 7 days from construction; H2O2 oxidation capability was also retained. Sulfone and sulfonate groups of Chemiplus 2DS HB are likely responsible for the dimensionality of the film and the electrostatic interaction leading to a self-blocking/self-rejection of AA. The way Pt/Chemiplus can reveal the AA presence depends on the maintaining of AA rejection, and this peculiarity can distinguish it from other sensors or biosensors.

ACS Style

Patrizia Monti; Quirico Migheli; Andrea R. Bartiromo; Antonio Pauciulo; Rocco Gliubizzi; Salvatore Marceddu; Pier A. Serra; Giovanna Delogu. A Storage-Dependent Platinum Functionalization with a Commercial Pre-Polymer Useful for Hydrogen Peroxide and Ascorbic Acid Detection. Sensors 2019, 19, 2435 .

AMA Style

Patrizia Monti, Quirico Migheli, Andrea R. Bartiromo, Antonio Pauciulo, Rocco Gliubizzi, Salvatore Marceddu, Pier A. Serra, Giovanna Delogu. A Storage-Dependent Platinum Functionalization with a Commercial Pre-Polymer Useful for Hydrogen Peroxide and Ascorbic Acid Detection. Sensors. 2019; 19 (11):2435.

Chicago/Turabian Style

Patrizia Monti; Quirico Migheli; Andrea R. Bartiromo; Antonio Pauciulo; Rocco Gliubizzi; Salvatore Marceddu; Pier A. Serra; Giovanna Delogu. 2019. "A Storage-Dependent Platinum Functionalization with a Commercial Pre-Polymer Useful for Hydrogen Peroxide and Ascorbic Acid Detection." Sensors 19, no. 11: 2435.

Journal article
Published: 29 April 2019 in Food Control
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This study aimed to explore the incidence of Fusarium fungi and their mycotoxins in marketed feed in Qatar. Samples of mixed-grain-cereal, maize, wheat, millet, barley, oat and soybean were tested for the presence of zearalenone (ZEN), fumonisins, T2/HT2 and deoxynevalenol (DON) mycotoxins. PCR-based identification of the Fusarium isolates was followed by investigation into genes involved in trichothecenes (tri gene cluster), fumonisins (fum gene cluster) and ZEN (pks gene) biosynthesis. In-vitro mycotoxin production potential of the isolates was compared with their molecular profiles. Mixed-grain cereals showed highest contamination with Fusarium, followed by maize and wheat. The incidence of ZEN was highest in mixed grain samples (87.5%) followed by maize (50%), millet (42.85%) and wheat (40%). In case of DON; 62.5%, 30%, 40%, 14.28%, 50% and 66.67% of the tested mixed cereal grain, maize, wheat, millet, barley and soybean samples were positive. Based on PCR results, F. verticillioides showed highest frequency distribution (34%), followed by F. graminearum (16%), F. oxysporum (15%), F. proliferatum (13%), F. culmorum (8%), F. solani (7%), F. subglutinans (4%) and F. avenaceum (3%) in the feed cereals. All the isolates of F. verticillioides presented both fum1 and fum13 genes, and produced fumonisin mycotoxins in-vitro. All the isolates of F. graminearum and F. culmorum had the tri5 and tri6 genes and resulted in DON accumulation in rice media. In addition to DON, all the F. culmorum isolates produced ZEN in artificially contaminated media, in line with their molecular profile of having tri5, tri6 and pks13 genes. The findings of present study confirmed the occurrence of toxigenic Fusarium and their mycotoxins in animal feed, all below the maximum limit set by EU. Further, the in-vitro mycotoxin production potential of the isolates was in agreement with their tri, fum and pks genetic profiles.

ACS Style

Zahoor Ul Hassan; Roda Al Thani; Virgilio Balmas; Quirico Migheli; Samir Jaoua. Prevalence of Fusarium fungi and their toxins in marketed feed. Food Control 2019, 104, 224 -230.

AMA Style

Zahoor Ul Hassan, Roda Al Thani, Virgilio Balmas, Quirico Migheli, Samir Jaoua. Prevalence of Fusarium fungi and their toxins in marketed feed. Food Control. 2019; 104 ():224-230.

Chicago/Turabian Style

Zahoor Ul Hassan; Roda Al Thani; Virgilio Balmas; Quirico Migheli; Samir Jaoua. 2019. "Prevalence of Fusarium fungi and their toxins in marketed feed." Food Control 104, no. : 224-230.

Journal article
Published: 01 November 2018 in International Journal of Food Microbiology
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Many foods and beverages in temperate and tropical regions are prone to contamination by ochratoxin A (OTA), one of the most harmful mycotoxins for human and animal health. Aspergillus ochraceus and Aspergillus carbonarius are considered among the main responsible for OTA contamination. We have previously demonstrated that four low or non- fermenting yeasts are able to control the growth and sporulation of OTA-producing Aspergilli both in vitro and on detached grape berries: the biocontrol effect was partly due to the release of volatile organic compounds (VOCs). Aiming to further characterise the effect of VOCs produced by biocontrol yeast strains, we observed that, beside vegetative growth and sporulation, the volatile compounds significantly reduced the production of OTA by two A. carbonarius and A. ochraceus isolates. Exposure to yeast VOCs also affected gene expression in both species, as confirmed by downregulation of polyketide synthase, non-ribosomal peptide synthase, monooxygenase, and the regulatory genes laeA and veA. The main compound of yeast VOCs was 2-phenylethanol, as detected by Headspace-Solid Phase Microextraction-Gas Chromatography-Tandem Mass Spectrometry (HS-SPME-GC–MS) analysis. Yeast VOCs represent a promising tool for the containment of growth and development of mycotoxigenic fungi, and a valuable aid to guarantee food safety and quality.

ACS Style

Maria Grazia Farbo; Pietro Paolo Urgeghe; Stefano Fiori; Angela Marcello; Stefania Oggiano; Virgilio Balmas; Zahoor Ul Hassan; Samir Jaoua; Quirico Migheli. Effect of yeast volatile organic compounds on ochratoxin A-producing Aspergillus carbonarius and A. ochraceus. International Journal of Food Microbiology 2018, 284, 1 -10.

AMA Style

Maria Grazia Farbo, Pietro Paolo Urgeghe, Stefano Fiori, Angela Marcello, Stefania Oggiano, Virgilio Balmas, Zahoor Ul Hassan, Samir Jaoua, Quirico Migheli. Effect of yeast volatile organic compounds on ochratoxin A-producing Aspergillus carbonarius and A. ochraceus. International Journal of Food Microbiology. 2018; 284 ():1-10.

Chicago/Turabian Style

Maria Grazia Farbo; Pietro Paolo Urgeghe; Stefano Fiori; Angela Marcello; Stefania Oggiano; Virgilio Balmas; Zahoor Ul Hassan; Samir Jaoua; Quirico Migheli. 2018. "Effect of yeast volatile organic compounds on ochratoxin A-producing Aspergillus carbonarius and A. ochraceus." International Journal of Food Microbiology 284, no. : 1-10.

Editorial
Published: 03 October 2018 in Biocontrol Science and Technology
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Prakya Sreerama Kumar; Quirico Migheli; Charles H. Pickett; Travis Glare. Editorial. Biocontrol Science and Technology 2018, 28, 913 -913.

AMA Style

Prakya Sreerama Kumar, Quirico Migheli, Charles H. Pickett, Travis Glare. Editorial. Biocontrol Science and Technology. 2018; 28 (10):913-913.

Chicago/Turabian Style

Prakya Sreerama Kumar; Quirico Migheli; Charles H. Pickett; Travis Glare. 2018. "Editorial." Biocontrol Science and Technology 28, no. 10: 913-913.

Journal article
Published: 01 October 2018 in Food Control
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This study was designed to explore the incidence of aflatoxin M1 (AFM1) in milk and dairy products marketed in Qatar. Milk (n=72), yogurt (n=21), cheese (n=46), butter (n=18) and laban (n=25) samples were initially screened by competitive enzyme linked immunosorbent assay (ELISA) for the presence of AFM1, followed by confirmation with ultra-high-performance liquid chromatography (UHPLC). AFM1 was detected in 85%, 76%, 85%, 67% and 76% of the milk, yogurt, cheese, butter and laban samples, respectively. The levels of AFM1 in pasteurized vs ultra-high temperature (UHT) milk and full- vs low-fat yogurts showed a non-significant difference. Halloumi and Kashkaval cheese varieties showed a significantly higher AFM1 contents as compared to Mozzarella, Edam, Cheddar, cream and Moshalal cheese. Likewise, unsalted butter demonstrated significantly (p<0.05) higher AFM1 values than salted butter samples. None of the tested samples presented AFM1 levels above the EU maximum limits of 50 ng/L or kg for milk, yogurt and butter, and 250 ng/kg for cheese. From the finding of present study, it can be concluded that, although high percentage of dairy products marketed in Qatar demonstrated AFM1 contents, but do not represent a public health concern considering the EU maximum limits.

ACS Style

Zahoor Ul Hassan; Roda Al-Thani; Fathy A. Atia; Saeed Almeer; Virgilio Balmas; Quirico Migheli; Samir Jaoua. Evidence of low levels of aflatoxin M1 in milk and dairy products marketed in Qatar. Food Control 2018, 92, 25 -29.

AMA Style

Zahoor Ul Hassan, Roda Al-Thani, Fathy A. Atia, Saeed Almeer, Virgilio Balmas, Quirico Migheli, Samir Jaoua. Evidence of low levels of aflatoxin M1 in milk and dairy products marketed in Qatar. Food Control. 2018; 92 ():25-29.

Chicago/Turabian Style

Zahoor Ul Hassan; Roda Al-Thani; Fathy A. Atia; Saeed Almeer; Virgilio Balmas; Quirico Migheli; Samir Jaoua. 2018. "Evidence of low levels of aflatoxin M1 in milk and dairy products marketed in Qatar." Food Control 92, no. : 25-29.

Journal article
Published: 31 August 2018 in Science of The Total Environment
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The fumigant 1,3-dichloropropene (1,3-D) is widely-used to control pathogenic bacteria, fungi, nematodes and insects in soil before a crop is planted. Although fumigants in general have been reported to have a ‘fertilizer effect’ in the soil by increasing nitrogen availability, little is known of how a specific fumigant such as 1,3-D affects available nitrogen. This study used real-time quantitative PCR (qPCR) and 16S rRNA gene amplicon sequencing techniques to investigate the effects of 1,3-D on microorganisms involved in nitrogen cycling that were present in 2 soils: Jiangxi lateritic red soil and Beijing fluvo-aquic soil. The fumigant 1,3-D temporarily decreased the abundance of 11 functional genes involved in nitrogen-fixing, nitrification and denitrification in both soil types. Different nitrogen cycling groups recovered to the unfumigated level in various incubation phases. Microorganisms containing nifH, nxrB, napA and qnorB genes were most vulnerable to 1,3-D fumigation. However, a stronger and longer inhibition effect of 1,3-D on these 11 functional genes was observed in Jiangxi soil than in Beijing soil. At the same time, the abundance of nifH, AOBamoA, nirS, qnorB and cnorB genes was significantly increased 59 days after 1,3-D fumigation. Fumigation with 1,3-D significantly reduced the nitrogen-fixing bacteria Azospirillum and Paenibacillus; the nitrifiers Nitrosomonas and Nitrospira; and the denitrifiers Pseudomonas, Paracoccus and Sphingomonas. Conversely, fumigation with 1,3-D increased the nitrogen-fixing bacteria Bradyrhizobium and Rhizobium; the nitrification bacteria Nitrosospira and Nitrolancea; and the denitrification bacteria Sphingobium, Alcanivorax, Bacillus, Streptomyces and Aeromonas. Fumigation with 1,3-D therefore caused significant shifts in the species composition and number of microbes directly involved in nitrogen cycling in the short-term. These results contribute toward a better understanding of the impact of 1,3-D fumigation on various types of soil nitrogen-cycling groups.

ACS Style

Wensheng Fang; Dongdong Yan; Qiuxia Wang; Bin Huang; Zongjie Ren; Xianli Wang; Xiaoning Wang; Yuan Li; Canbin Ouyang; Quirico Migheli; Aocheng Cao. Changes in the abundance and community composition of different nitrogen cycling groups in response to fumigation with 1,3-dichloropropene. Science of The Total Environment 2018, 650, 44 -55.

AMA Style

Wensheng Fang, Dongdong Yan, Qiuxia Wang, Bin Huang, Zongjie Ren, Xianli Wang, Xiaoning Wang, Yuan Li, Canbin Ouyang, Quirico Migheli, Aocheng Cao. Changes in the abundance and community composition of different nitrogen cycling groups in response to fumigation with 1,3-dichloropropene. Science of The Total Environment. 2018; 650 ():44-55.

Chicago/Turabian Style

Wensheng Fang; Dongdong Yan; Qiuxia Wang; Bin Huang; Zongjie Ren; Xianli Wang; Xiaoning Wang; Yuan Li; Canbin Ouyang; Quirico Migheli; Aocheng Cao. 2018. "Changes in the abundance and community composition of different nitrogen cycling groups in response to fumigation with 1,3-dichloropropene." Science of The Total Environment 650, no. : 44-55.

Journal article
Published: 10 April 2018 in Talanta
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Interference rejection in amperometric biosensors can be more effective introducing some modifiers during electro-deposition of permselective film. Addition of β-cyclodextrin (βCD), a cyclic oligosaccharide composed of seven glucose units, to the ortho-phenylendiamine (oPD) monomer were already demonstrated to provide an enhancement in ascorbic acid (AA) rejection. Here we evaluated the improvement in permselectivity of poly-eugenol and poly-magnolol films electro-polymerized in presence of different amounts of βCD or eugenol-βCD inclusion complex for amperometric biosensor application. Starting from Pt-Ir wire as transducer several microsensors were covered with polymeric films doped with βCD-based modifiers through constant potential amperometry. Characterization of modified polymers was achieved by scanning electron microscopy and permselectivity analysis. Poly-magnolol film in combination with βCD showed a worsening in permselectivity compared to poly-magnolol alone. In contrast, the introduction of βCD-based modifier enhanced the interference rejection toward the archetypal interferent AA, while slightly affecting permeability toward H2O2 compared to the poly-eugenol without modifier. The AA rejection seems to be influenced by the availability of βCD cavity as well as film performance due to concentration of βCD-Eugenol inclusion complex. A poly-eugenol film co-polymerized with 2 mM βCD-eugenol inclusion complex showed a permselectivity equal to poly-orthophenylendiamine film (PPD), with a lower permeability to AA, likely to be related with a self-blocking mechanism. Based on these results, a biosensor for glutamate was constructed with a poly-eugenol doped with βCD-eugenol as permselective layer and its permselectivity, stability and lifetime were determined.

ACS Style

Patrizia Monti; Gaia Rocchitta; Salvatore Marceddu; Maria A. Dettori; Davide Fabbri; Samir Jaoua; Quirico Migheli; Giovanna Delogu; Pier A. Serra. Use of β-cyclodextrin as enhancer of ascorbic acid rejection in permselective films for amperometric biosensor applications. Talanta 2018, 186, 53 -59.

AMA Style

Patrizia Monti, Gaia Rocchitta, Salvatore Marceddu, Maria A. Dettori, Davide Fabbri, Samir Jaoua, Quirico Migheli, Giovanna Delogu, Pier A. Serra. Use of β-cyclodextrin as enhancer of ascorbic acid rejection in permselective films for amperometric biosensor applications. Talanta. 2018; 186 ():53-59.

Chicago/Turabian Style

Patrizia Monti; Gaia Rocchitta; Salvatore Marceddu; Maria A. Dettori; Davide Fabbri; Samir Jaoua; Quirico Migheli; Giovanna Delogu; Pier A. Serra. 2018. "Use of β-cyclodextrin as enhancer of ascorbic acid rejection in permselective films for amperometric biosensor applications." Talanta 186, no. : 53-59.

Journal article
Published: 01 February 2018 in Food Control
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Zahoor Ul Hassan; Roda Fahad Al-Thani; Quirico Migheli; Samir Jaoua. Detection of toxigenic mycobiota and mycotoxins in cereal feed market. Food Control 2018, 84, 389 -394.

AMA Style

Zahoor Ul Hassan, Roda Fahad Al-Thani, Quirico Migheli, Samir Jaoua. Detection of toxigenic mycobiota and mycotoxins in cereal feed market. Food Control. 2018; 84 ():389-394.

Chicago/Turabian Style

Zahoor Ul Hassan; Roda Fahad Al-Thani; Quirico Migheli; Samir Jaoua. 2018. "Detection of toxigenic mycobiota and mycotoxins in cereal feed market." Food Control 84, no. : 389-394.

Journal article
Published: 31 May 2017 in Molecular Plant Pathology
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Fusarium culmorum is a soil‐borne fungal pathogen which causes foot and root rot and Fusarium head blight on small‐grain cereals, in particular wheat and barley. It causes significant yield and quality losses and results in the contamination of kernels with type B trichothecene mycotoxins. Our knowledge of the pathogenicity factors of this fungus is still limited. A transposon tagging approach based on the mimp1/impala double‐component system has allowed us to select a mutant altered in multiple metabolic and morphological processes, trichothecene production and virulence. The flanking regions of mimp1 were used to seek homologies in the F. culmorum genome, and revealed that mimp1 had reinserted within the last exon of a gene encoding a hypothetical protein of 318 amino acids which contains a ROGDI‐like leucine zipper domain, supposedly playing a protein–protein interaction or regulatory role. By functional complementation and bioinformatic analysis, we characterized the gene as the yeast Rav2 homologue, confirming the high level of divergence in multicellular fungi. Deletion of FcRav2 or its orthologous gene in F. graminearum highlighted its ability to influence a number of functions, including virulence, trichothecene type B biosynthesis, resistance to azoles and resistance to osmotic and oxidative stress. Our results indicate that the FcRav2 protein (and possibly the RAVE complex as a whole) may become a suitable target for new antifungal drug development or the plant‐mediated resistance response in filamentous fungi of agricultural interest.

ACS Style

Francesca Spanu; Barbara Scherm; Irene Camboni; Virgilio Balmas; Giovanna Pani; Safa Oufensou; Nicolo’ Macciotta; Matias Pasquali; Quirico Migheli. FcRav2, a gene with a ROGDI domain involved in Fusarium head blight and crown rot on durum wheat caused by Fusarium culmorum. Molecular Plant Pathology 2017, 19, 677 -688.

AMA Style

Francesca Spanu, Barbara Scherm, Irene Camboni, Virgilio Balmas, Giovanna Pani, Safa Oufensou, Nicolo’ Macciotta, Matias Pasquali, Quirico Migheli. FcRav2, a gene with a ROGDI domain involved in Fusarium head blight and crown rot on durum wheat caused by Fusarium culmorum. Molecular Plant Pathology. 2017; 19 (3):677-688.

Chicago/Turabian Style

Francesca Spanu; Barbara Scherm; Irene Camboni; Virgilio Balmas; Giovanna Pani; Safa Oufensou; Nicolo’ Macciotta; Matias Pasquali; Quirico Migheli. 2017. "FcRav2, a gene with a ROGDI domain involved in Fusarium head blight and crown rot on durum wheat caused by Fusarium culmorum." Molecular Plant Pathology 19, no. 3: 677-688.