Some Aedinii mosquitoes are of high importance in the transmission of the sylvatic YFV. Usually, their eggs are very resistant and depend on the rain for their hatching. The present study evaluated the effect of multiple mosquito-egg immersions and the sex ratio of male and female specimens from Atlantic Forest remnants in the state of Rio de Janeiro, Brazil. Three sampling sites were selected in the municipality of Casimiro de Abreu, where 50 ovitraps were randomly installed to collect eggs from the ground level up to different heights, from August 2018 to December 2020. The mosquito sex ratios were compared between seasons and forest sites, using the generalized linear mixed model (GLMM), which included sampling months and trees as random effects. A total of 33,091 mosquito eggs were collected, of which 6152 eggs were already hatched (18%) and 26,939 were unhatched; of these, approximately 76% subsequently hatched. We found that 25% of the eggs corresponded to four species: Aedes albopictus (n = 1277), Ae. terrens (n = 793), Haemagogus janthinomys (n = 89), and Hg. leucocelaenus (n = 3033). The sex ratio (male:female) was variable concerning the sampling sites and the season. For most species, GLMM estimates found no difference in the variation of the average sex ratio as a function of these predictors, and there was no evidence of temporal autocorrelation in the mosquito data. The number of immersions necessary for hatching the eggs differed between mosquito species, and eggs collected in the dry season hatched both in the first immersions and the subsequent events. Co-occurrence of Aedes terrens and Hg. leucocelaenus was the most frequently observed pairwise species combination. Considering recurrent arbovirus outbreaks in Brazil and their burden on the human population, our study helps to shed light on how these vectors behave in nature; therefore, they can be used in surveillance programs.

Diverse larval habitats significantly influence female mosquito oviposition. Utilizing traps that simulate these habitats is helpful in the study of the bioecology and characteristics of pathogen-transmitting species during oviposition. This study evaluated the feasibility of different traps in natural environments by comparing sampling methods and detecting the oviposition of epidemiologically important mosquitoes, with emphasis on Haemagogus species, in a fragment of the Atlantic Forest in Silva Jardim, Rio de Janeiro State, Brazil. Monthly collections were conducted from March 2021 to October 2023 using four types of traps: plastic containers, tires, bamboo, and sapucaia. Immatures were collected from these traps using a pipette, placed in plastic bags, and transported to the laboratory. Tire was the most efficient trap, showing the highest mosquito abundance (n = 1239) and number of species (S = 11). Conversely, the plastic container trap exhibited the lowest diversity (H = 0.43), with only two species and a low mosquito abundance (n = 26). The bamboo trap captured six species and recorded the second-highest diversity index (H = 1.04), while the sapucaia trap captured five species and had the third-highest diversity index (H = 0.91). Of the total immatures collected, 1817 reached adulthood, comprising 13 species, two of which are vectors of the sylvatic yellow fever virus: Haemagogus leucocelaenus and Haemagogus janthinomys. In conclusion, detecting key vectors of the sylvatic yellow fever virus in Brazil highlights the need for ongoing entomological and epidemiological surveillance in the study area and its vicinity. These efforts are crucial for monitoring vector presence and activity, identifying potential transmission hotspots, and devising effective control and prevention strategies.

Evaluating the development process of mosquito species under the influence of temperature is essential for understanding their ecology and geographical distribution, as well as assessing their potential as vectors of pathogens. Aedes (Protomacleaya) terrens, a species recognized for its susceptibility and competence in transmitting the chikungunya virus, serves as a relevant model for research in this context. This study aimed to analyze the influence of temperature on egg hatching and the development cycle of this species to expand knowledge on its biology and implications for public health. During the experiment, 800 eggs were used, collected through 10 ovitraps in a forest remnant located in Uruaçu, Goiás, Brazil. The total number of eggs was divided into four groups, exposed to constant temperatures of 15 ± 2 °C, 20 ± 2 °C, 25 ± 2 °C, and 30 ± 2 °C. After hatching, first-instar larvae were individually separated and monitored daily under controlled conditions until adult emergence. The highest hatching rate occurred at 25 °C, showing an optimal point around 27 °C. Throughout development, temperature significantly reduced the duration of each stage, with the fastest complete cycle at 30 °C, a difference of approximately 10–12 days when compared to 20 °C and approximately 47 days when compared to 25 °C. These results offer valuable insights into the temperature sensitivity of Ae. terrens across its developmental stages, suggesting that each stage has its own optimal temperature. Thus, small variations in responses to environmental conditions and differentiation between sexes may become more pronounced throughout development. In this sense, temperature can affect not only the development and survival of dipterans but also the capacity for virus transmission, as the pathogen influences the reproduction rate and longevity of the vectors.