In the present investigation, commercially pure titanium is welded to AISI 316L stainless steel by intermixing niobium as filler material in a lap joint configuration. For this purpose, a pulsed Nd:YAG laser with various pulse durations and pulse peak powers is employed to obtain different mixing conditions for the materials. It will be demonstrated that, despite the implementation of the filler material, the weld seams are characterized by a high affinity for cracking, which in turn can be attributed to the formation of hard intermetallic compounds. Nevertheless, utilization of optimized process parameters can yield crack-free specimens in a reproducible manner through equable intermixing of otherwise critical alloy elements. Lap-shear forces of up to 140 N can be achieved with a single weld seam measuring 2.5 mm in length. By increasing the joint area with four adjacent weld seams, maximum loads up to 320 N are attained, thus exceeding the yield strength of the applied stainless steel. Considering the biocompatibility of the niobium filler material used, this work provides the foundation for this dissimilar material combination to be implemented in future medical technology applications.