In the next twenty years, the scarcity of food shortage and drinking water will appear in Egypt due to the growth of industries and agriculture. This paper develops a conceptual design of the new technologies in the field of water–energy–food in new cities. Border lines are the internal relationship, external influence, and linkage system evaluation for WEF nexus. The major problems of using fossil energy in desalination are emissions and non-renewability, as well as the preference for dispersed freshwater production instead of concentrated output. The design of a desalination system that is integrated with renewable energies is critical these days. This type of system can also reduce the production of environmental pollutants due to reduced energy consumption and transfer of freshwater. GIS data from the United Nations have confirmed the existence of an underground reservoir in Wadi-Dara that can cultivate 1000 acres using smart farming techniques to reach a circular economy for an integrated solution between the water–energy nexus. The possibility of cultivating a hundred acres in Wadi-Dara on the Red Sea coast exists, through which about one million people could be settled. In this comprehensive review, we conducted a deep study in order to establish a sustainable integrated lifestyle in the Dara Valley region in terms of the availability of potable water, clean energy, and agriculture. Sustainable integrated solutions were conducted for seawater desalination using beach sand filtration wells as a pretreatment for seawater using renewable energy, e.g., wind energy (18% wind turbines), and photovoltaic panels (77% PV panels). Strategic food will be cultivated using smart farming that includes an open ponds cultivation system of microalgal cells to synthesis (5.0% of bio-fuel (. Aqua agriculture and aquaponics will cultivate marine culture and integrate mangrove, a shrimp aquaculture. A municipal waste water treatment is conceived for the irrigation of shrubby forests and landscapes. Mixotrophic cultures were explored to achieve a sustained ecological balance. Food, poultry and animal waste management, as well as a cooker factory, were included in the overall design. The environmental impact assessment (EIA) study shows a low risk due to anticipated net zero emissions, a 75% green city, and optimal waste recycling. This research assists in combining research efforts to address the challenging processes in nexus research and build resilient and sustainable water, energy, and food systems.

Escherichia coli is a major cause of community‐acquired urinary tract infections (CA‐UTIs). In this study, we investigated the antimicrobial resistance patterns, the distribution of phylogenetic groups, and the prevalence and characteristics of integron-bearing E. coli isolates from outpatients with CA‐UTIs in El-Minia governorate, in Upper Egypt. Out of the 583 urine samples collected, 134 were positive for E. coli, from which the most resistant isolates (n = 80) were selected for further analysis. The majority of these isolates (62.5%, 50/80) showed multidrug resistance profiles. Group B2 was the most predominant phylogenetic group (52.5%), followed by group F (21.25%), Clades I or II (12.5%), and finally isolates of unknown phylogroup (13.75%). Of the 80 isolates, 7 (8.75%) carried class 1 integrons, which contained 3 different types of integrated gene cassettes, including those conferring resistance to streptomycin/spectinomycin, trimethoprim, and some open reading frames of unknown function (gcuF). In conclusion, the types and combinations of the gene cassettes in our study may reflect the specific selective pressures to which the isolates were subjected within the study region, therefore, providing valuable data for future intervention strategies that are precisely tailored to prevent the dissemination of the uropathogenic E. coli strains circulating within Upper Egypt.

This study targeted developing a novel Zinc oxide with alpha hematite nanoflowers (NFs)-loaded bee venom (Bv) (Bv-ZnO@αFe₂O₃ NFs) as a bio-natural product from bees to combine both the advantages of combination magnetic properties and the antimicrobial and anti-biofilm properties on isolated coliform bacteria from the effluent of wastewater treatment plants. About 24 isolates of treated wastewater isolates were multidrug resistant (MDR). The phylogenetic grouping of Escherichia coli (E. coli) and Klebsiella pneumonia (K. pneumonia) showed that the largest group was Group A, followed by Group B2 and Group B1. Fourier transform infrared (FTIR), The X-ray diffraction (XRD), and scanning electron microscopy-energy dispersive X-ray analysis (SEM− EDX) validated the coating operation’s contact with Bv onto ZnO@αFe₂O₃ NFs. According to high-resolution transmission electron microscopy (TEM) and selected area electron diffraction (SAED), pattern analyses for prepared nanoformulations exhibited a spherical shape of αFe₂O₃ (~9–15 nm), and floral needle shapes with uniform distribution of size with aggregation of ZnOαFe₂O₃ and Bv-ZnO@αFe₂O₃ NFs around (~100–200 nm). The toxicity of Bv-ZnO@αFe₂O₃ NFs was comparable up to 125 µg mL⁻¹, when it reached 64.79% (IC₅₀, 107.18 µg mL⁻¹). The antibacterial activity showed different zones of inhibition against different isolates. The biofilm inhibitory activity of NPs and NFs showed a highly significant reduction (p < 0.001) in treated biofilms with ZnO@αFe₂O₃ and Bv-ZnO@αFe₂O₃. In essence, ZnO@αFe₂O₃ and Bv-ZnO@αFe₂O₃ NFs are promising antimicrobials for inhibiting the growth and biofilm of MDR E. coli and K. pneumonia isolates, thereby, biocontrol of wastewater.