We demonstrate the possibility of applying surface-enhanced Raman spectroscopy (SERS) combined with machine learning technology to detect and differentiate influenza type A and B viruses in a buffer environment. The SERS spectra of the influenza viruses do not possess specific peaks that allow for their straight classification and detection. Machine learning technologies (particularly, the support vector machine method) enabled the differentiation of samples containing influenza A and B viruses using SERS with an accuracy of 93% at a concentration of 200 μg/mL. The minimum detectable concentration of the virus in the sample using the proposed approach was ~0.05 μg/mL of protein (according to the Lowry protein assay), and the detection accuracy of a sample with this pathogen concentration was 84%.

Productivity and nutritional value of poaceous crops sown as a single crop and intercropped with spring rape have been studied. Field and laboratory studies were conducted in 2012–2014 on meadow chernozem mealy-carbonate soil in the forest-steppezone of Trans-BaikalTerritory. Agricultural technology used for fodder crop cultivation was common for this area. The objects of the research were the following recognized varieties: oats Metis, spring rye Onokhoiskaya, spring triticale Ukro, spring rape Shpat. The experimental work was carried out in accordance with the generally accepted guidelines for fi eld experiments. Poaceous crops and spring rape were assessed in terms of their adaptability to growing conditions and their economically valuable characteristics were shown. The possibility of increasing the productivity and nutritional value of fodder agrocenoses by intercropping spring rape with poaceous crops was established. In multi-crop agrocenoses, the yield increased and the quality of feed raw material improved. The best results of agrocenoses in multi-crop sowings were achieved by spring triticale intercropped with spring rape and oats intercropped with spring rape: the yield of green mass was 22.1-23.5 t/ha, the amount of dry matter 5.09-5.19, feed units 3.65-3.83 t/ha, digestible protein 598.6-654.9 kg/ha, gross energy 54.055.5 GJ/ha, availability of digestible protein164171 gper feed unit. In terms of productivity, spring triticale and oats intercropped with spring rape surpassed single-crop poaceous agrocenoses by the following parameters: feed units by 1.1-1.9 times, digestible protein by 1.8-3.6 times, gross energy by 1.3-2.6 times, with 10.6-10.7 MJ of dry matter in one kilogram of exchangeable energy.

The results of field and laboratory research for 2015–2017 are presented and analyzed on the cultivation of traditional and uncommon poaceous crops. Recognized varieties of barley, oats, and millet were studied in single-crop sowings and intercropped with a high-protein cabbage crop – oilseed radish. The research was conducted on meadow chernozem mealy-carbonate soil in the forest-steppe zone of Trans-Baikal Territory. Agricultural technology used for fodder crop cultivation was common for this area. The objects of the research were the following varieties: barley Anna, oats Metis, millet Bystroe, oilseed radish Tambovchanka. The experimental work was carried out in accordance with the generally accepted guidelines for field experiments. Poaceous crops and oilseed radish were assessed in terms of their adaptability to growing conditions and their economically valuable characteristics that ensure sustainable production of high-energy feed. In multi-crop agrocenoses of poaceous crops and oilseed radish, the yield increased and the quality of feed raw material improved. The best results of multi-crop sowings were achieved by oats and millet intercropped with oilseed radish. The yield of green mass was 38.8-39.7 t/ha, dry matter content – 4.88-4.90 t/ha, digestible protein – 810820 kg/ha, and gross energy – 46.3-52.2 GJ/ha with availability of digestible protein – 180-186 g per one feed unit. Multi-crop sowings of oats and millet with radish oilseed are more productive than single-crop poaceous agrocenoses in feed units by 1.5-1.7 times, digestible protein by 2.9-3.3 times, gross energy by 1.2-1.6 times, with 10.5-10.7 MJ of exchangeable energy in one kilogram of dry matter.