The only cells in an organism that could do any other sort of cell until 2006 (except sperm or egg) were known as embryonic stem cells, ESC

This study investigates the chemical composition of soyhulls (SHs) as an alternative feed ingredient and their effect on nutrient and amino acid (AA) digestibility in laying hens during peak production. A total of 200 golden brown hens (28 weeks old) were subjected to random allocation across 5 dietary treatments: a corn–soybean meal (SBM) reference diet and 4 test diets with 25% SHs from different mills (SH1, SH2, SH3, and SH4). Each treatment was replicated four times with ten birds per replicate. Digesta samples were collected during three phases (28–32, 32–36, and 36–40 weeks of age) to measure apparent metabolizable energy (AME), the apparent ileal digestibility (AID) of nutrients, and the standard ileal digestibility (SID) of AAs. The SBM diet had 30.0% crude protein (CP) and 3.78% crude fiber (CF), while the SH diets had 21.0 to 21.5% CP and 11.6% CF. The findings revealed that the AME was lower (p < 0.05) with SH diets (2404 kcal/kg) compared to the SBM diet (2627 kcal/kg) in all three phases. The SH diets had a lower AID of dry matter (DM), crude protein (CP), ash, ether extract (EE), and crude fiber (CF) than the SBM diet by an average of 2.88, 2.25, 4.93, 4.99, and 3.36%, respectively. The AID of nitrogen-free extract (NFE) was higher in the SH diets than the SBM diet by 3.42% in all three phases (p < 0.05). The SH diets had lower uric acid excretion (about 66.93 mg/100 mL) than the SBM diet (about 76.43 mg/100 mL) on average in all three phases. The SH diets had a lower SID of arginine, histidine, isoleucine, lysine, cysteine, valine, and tyrosine than the SBM diet by 2 to 10%, while the SID of methionine was higher in the SH diets than the SBM diet by 2.2% on average in all three phases (p < 0.05). The SH from Sadiq Brother Feed (SH1) had the highest AME and AID of DM, ash, CP, EE, CF, and the SID of AA among the SH diets. These results indicate that SH can partially replace SBM in laying hen diets, but the source and quality of SH should be considered.

Globally, ruminants contribute largely to the livelihood and supply of quality food for human consumption. However, small ruminants face numerous problems, including infectious diseases, in lower- and middle-income countries (LMIC). Brucellosis is one of the important zoonotic diseases affecting the range of animals caused by Brucella species, including Brucella abortus and Brucella mellitensis. Although brucellosis caused by B. mellitensis in small ruminants has never been reported in the study areas, its zoonotic importance can never be underestimated. Therefore, this study was designed to investigate the sero-molecular prevalence of B. mellitensis in small ruminants in districts Mohmand and Charsadda of Khyber Pakhtunkhwa, Pakistan. A total of 400 blood samples were collected from sheep and goats (n = 200 from each species) and analyzed by Rose Bengal precipitation test (RBPT), the indirect enzyme-linked immunosorbent assay (i-ELISA) and polymerase chain reaction (PCR). The findings of the study indicated 13.5% and 7% of sheep while 12.5% and 12.5% of goat’s samples by RBPT and (i-ELISA) respectively. The species-specific PCR confirmed B. abortus in 70% of sheep samples and 37.5% of goat’s samples and B. mellitensis in 25% of sheep and 62.5% of goat’s samples by targeting IS711. The findings of the study concluded that B. abortus and B. melitensis were circulating in sheep and goats with a higher prevalence in the study areas. This study detected the presence of B. mellitensis for the first time in small ruminants in the study areas.