Due to the spatial network structure, heavy oil has a high threshold pressure gradient when it flows through porous media, and the threshold pressure gradient plays a crucial role in the distribution of remaining oil. In previous study, the common methods to measure the threshold pressure gradient include the microflow-established differential pressure (MFEDP) method, capillary equilibrium method, and the percolation curve fitting method. In this study, a sample from the SZ36-1 oilfield was analyzed for the basic physical properties based on the comparison of the previous measurement to study the influence of mobility on the threshold pressure gradient and then an independently developed numerical simulator was established to study the effect of the threshold pressure gradient on the remaining oil distribution considering the permeability range, crude oil viscosity, well network deployment, well spacing, and fluid recovery rate. The results show that the SZ36-1 oilfield fluid belongs to Bingham fluids with yield stress and the mobility having an exponent relation to the threshold pressure gradient based on the measurement of the MFEDP method. Considering the threshold pressure gradient of heavy oil, the uneven distribution of remaining oil is intensified and the remaining oil is enriched. This study provides a reference for efficient development of heavy oil reservoirs.