Super-resolution vibrational microscopy is promising to increase the degree of multiplexing of nanometer-scale biological imaging because of the narrower spectral linewidth of molecular vibration compared to fluorescence. However, current techniques of super-resolution vibrational microscopy suffer from various limitations including the need for cell fixation, high power loading, or complicated detection schemes. Here, we present reversible saturable optical Raman transitions (RESORT) microscopy, which overcomes these limitations by using photoswitchable stimulated Raman scattering (SRS). We first describe a bright photoswitchable Raman probe (DAE620) and validate its signal activation and depletion characteristics when exposed to low-power (microwatt level) continuous-wave laser light. By harnessing the SRS signal depletion of DAE620 through a donut-shaped beam, we demonstrate super-resolution vibrational imaging of mammalian cells with excellent chemical specificity and spatial resolution beyond the optical diffraction limit. Our results indicate RESORT microscopy to be an effective tool with high potential for multiplexed super-resolution imaging of live cells.

In-plane moving MEMS bistable elements with symmetrical stable states can be realized using post-process compression of a straight beam to be made buckle. This paper derives and experimentally demonstrates an analytical theory of this element. Design parameters of the component structures, a buckling beam, supporting arms, and compression mechanisms with a receptacle that can be made flexible are related to the behavior including the beam’s profile, the force required to make a flip to the other state, and the displacement where the flip happens. Test devices were fabricated using the silicon-on-insulator MEMS technique and their response to an electrostatic attraction was measured. The experimental results matched the developed theory. The theory and the experimental results presented in this paper can facilitate the introduction of symmetrical bistable structures as a new mechanical element in future MEMS devices. [2025-0002]

Atmospheric pressure measurements based on microelectromechanical systems (MEMSs) can extend accessibility to altitude information. A differential pressure sensor using a thin cantilever and an air chamber is a promising sensing element for sub-centimeter resolution. However, its vulnerability to wind and the lack of height estimation algorithms for real-time operation are issues that remain to be solved. We propose a sensor “cap” that cancels the wind effect and noise by utilizing the airflow around a sphere. A set of holes on the spherical cap transmits only the atmospheric pressure to the sensor. In addition, we have developed a height estimation method based on a discrete transfer function model. As a result, both dynamic pressure and noise are suppressed, and height is estimated under a 5 m/s wind, reconstructing the trajectory with an estimation error of 2.8 cm. The developed sensing system enhances height information in outdoor applications such as unmanned aerial vehicles and wave height measurements.

This document provides a design description of a data acquisition device that allows an alert to be issued to suggest to the driver to take a break after having subjected his body to a certain amount of acceleration and pressure changes after driving on a road with too many curves. The tests were carried out using sensors based on microelectromechanical systems. The system was strategically installed at specific points on the body of the driver and car. Several electronic arrays were carried out, like the design of a printed circuit board. The establishment of an inter-integrated circuit communication and its multiplexing to work with several devices with the same address simultaneously. Finally, in this context, the document also presents the critical velocity for each curve in the Hakone roadway, which was obtained by using a mathematical model and contrasted with data acquisition values for acceleration. The risk of skidding on a curve increases when the driver does not reduce driving velocity; only a slight variance in acceleration or environmental conditions is enough. The value of acceleration was acquired for the analysis of each curve; there is a greater possibility of skidding in curves 2 and 4 because their radius is smaller and the critical speed is approximately 60 km/h, which is very close to the driving speed. On the other hand, the deceleration value of −0.65 G read on the head accelerometer can increase fatigue symptoms such as blurry vision or dizziness.