The 3D (three-dimensional) micro–nanostructured diatom biosilica obtained from cultivated diatoms was used as a support to immobilize epitaxially growing AgCl-Ag hybrid nanoparticles ((Ag-AgCl)NPs) for the synthesis of nanocomposites with antimicrobial properties. The prepared composites that contained epitaxially grown (Ag-AgCl)NPs were investigated in terms of their morphological and structural characteristics, elemental and mineral composition, crystalline forms, zeta potential, and photoluminescence properties using a variety of instrumental methods including SEM (scanning electron microscopy), TEM (transmission electron microscopy), EDX (energy-dispersive X-ray spectroscopy), XRD (X-ray powder diffraction), zeta-potential measurement, and photoluminescence spectroscopy. The content of (AgCl-Ag)NPs in the hybrid composites amounted to 4.6 mg/g and 8.4 mg/g with AgClNPs/AgNPs ratios as a percentage of 86/14 and 51/49, respectively. Hybrid nanoparticles were evenly dispersed with a dominant size of 5 to 25 nm in composite with an amount of 8.4 mg/g of silver. The average size of the nanoparticles was 7.5 nm; also, there were nanoparticles with a size of 1–2 nm and particles that were 20–40 nm. The synthesis of (Ag-AgCl)NPs and their potential mechanism were studied. The MIC (the minimum inhibitory concentration method) approach was used to investigate the antimicrobial activity against microorganisms Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus. The nanocomposites containing (Ag-AgCl)NPs and natural diatom biosilica showed resistance to bacterial strains from the American Type Cultures Collection and clinical isolates (diabetic foot infection and wound isolates).

Clay minerals are actively used to obtain a bioactive composite. Kaolinite, as a representative of clay minerals, possesses unique properties essential for the creation of biocomposite materials. This mineral, characterized by its distinctive layered structure, is chemically inert, highly stable, thermally resistant, eco-friendly, biocompatible, and non-toxic. Kaolinite, which plays the role of a carrier in this work, has such properties and can be the basis for biologically active composites. Antibacterial composites, namely, kaolinite/chlorhexidine and kaolinite/triclosan, were synthesized by impregnation of calcined and non-calcined samples of natural kaolinite with the antibacterial agents chlorhexidine and triclosan. The structure, morphology, elemental composition, and mineralogical characteristics of the natural and synthesized kaolinite/chlorhexidine (KAO/CHX) and kaolinite/triclosan (KAO/TCS) composites were investigated by methods of analysis such as X-ray diffraction, FTIR (Fourier-transform infrared) spectroscopy, and scanning electron microscopy. The calcined kaolinite/chlorhexidine composite at 500 °C (KAO_500°C/CHX) exhibited a higher content of antiseptics compared to the non-calcined kaolinite composite. The antibacterial activities of the kaolinite/chlorhexidine and kaolinite/triclosan composites were investigated against Gram-positive Staphylococcus epidermidis and Gram-negative Klebsiella pneumoniae and Escherichia coli strains by the well diffusion method and dilution method. The highest zone of inhibition was observed against Staphylococcus epidermidis (30.00 ± 0.00 mm and 30.67 ± 0.58 mm) by applying KAO/TCS and KAO_500°C/TCS via the well diffusion method. The minimum bactericidal concentration of the kaolinite/TCS composite was 15.63 μg/mL for Staphylococcus epidermidis and Klebsiella pneumoniae.