Exploring the relationship between regional ecosystem service supply and demand and establishing refined ecological management zoning can help enhance the rational allocation and conservation capacity of ecosystem services. Taking Anxi County as the study area, this study integrates the InVEST model, a supply-demand matching index, and the XGBoost-SHAP method to examine the spatiotemporal characteristics and driving mechanisms of ecosystem service supply-demand matching from 2000 to 2020 and to delineate ecological management zones from a combined dynamic-static perspective. The results indicate that: (1) From 2000 to 2020, the supply of multiple ecosystem services in Anxi County generally exhibited a spatial pattern of higher levels in the west and lower levels in the east. Among them, the supply of water yield and soil retention first decreased and then increased, whereas habitat quality and carbon storage generally declined to varying degrees. Demand for all four ecosystem services increased to varying degrees. (2) From 2000 to 2020, the mean values of the four ecosystem service supply-demand ratios in Anxi County declined to varying degrees. Among them, the supply-demand ratios of habitat quality (HQ), carbon sequestration (CS), and water yield (WY) generally exhibited a spatial pattern of higher values in the west and lower values in the east, whereas the soil retention (SR) supply-demand ratio showed an opposite pattern with higher values in the east and lower values in the west. (3) The nighttime light index, elevation, GDP density, and mean annual temperature were the core variables influencing the integrated ecosystem service supply–demand matching, with the nighttime light index contributing the most; GDP density and population density generally showed negative effects, whereas elevation, NDVI, and mean annual precipitation exhibited positive effects to varying degrees. Significant nonlinear and interactive effects were observed among the driving factors. (4) At the village scale, Anxi County is divided into three ecological management zones, agricultural production zones, resilient development zones, and ecological conservation zones with differentiated management strategies implemented. This study improves the rationality and efficiency of regional resource allocation and provides a scientific basis for local governments to formulate ecological protection policies, strengthen ecological security, and implement ecological compensation mechanisms.