Lactococcus lactis subsp. lactis exhibits probiotic properties in humans. Considering that Caenorhabditis elegans can be used to study the effects of microorganisms on animal behavior, owing to its simple nervous system, we assessed the impacts of two strains of Lactococcus lactis subsp. Lactis—a non-nisin-producing strain, NBRC 100933 (LL100933), and a nisin-producing strain, NBRC 12007 (LL12007)—on the lifespan, locomotion, reproductive capacity of, and lipid accumulation in, C. elegans. The lifespan of adult C. elegans fed a mixture (1:1) of Escherichia coli OP50 and LL100933 or LL12007 did not show a significant increase compared to that of the group fed a standard diet of E. coli OP50. However, the nematodes fed Lactococcus strains showed notable enhancement in their locomotion at all of the tested ages. Further, the beneficial effects of LL100933 and LL12007 were observed in the daf-16 mutants, but not in the skn-1 and pmk-1 mutants. The lipid accumulation in the worms of the Lactococcus-fed group was lower than that in the control group at all experimental ages. Overall, LL100933 and LL12007 enhance the locomotor behavior of C. elegans, likely by modulating the PMK-1/p38 MAPK and SKN-1/Nrf2 transcription factors.

This study was carried out to examine the prevalence of AFM1 contamination across different areas of Chattogram, Bangladesh, and to assess the level of AFM1 in raw milk samples from various dairy farms. A cross-sectional study was conducted to assess the farming standards of three different Chattogram neighborhoods—Bakalia, Khulshi, and Pahartali—and to ascertain the amount of AFM1 in milk. In the study location, 30 commercial dairy farms were randomly chosen, and data on farming methods, milk production techniques, and knowledge of aflatoxin contamination were gathered. Using the enzyme-linked immunosorbent assay (ELISA) method, collected milk samples (n = 90) were examined for the presence of AFM1. The farms produced 71.67±14.71 liters of milk on average every day, which was primarily supplied to dairy processing plants. For feeding the cows, all farms used concentrates and forage.  The mean concentration of AFM1 in milk samples collected from Bakalia was higher (190.00±120.87 ng/L) than that in milk samples collected from the Khulshi (108.44±66.19 ng/L) and Pahartali (189.25±160.78 ng/L). The overall prevalence of AFM1 was 43% (N=39) of the total examined samples. A total of 69% (N=27) and 5% (N=2) of AFM1 positive samples exceeded the European Union Regulation (50 ng/L) and BSTI/BFSA regulations (500 ng/L) respectively. There was a significant difference in the occurrence of AFM1 in Bakalia regarding Khulshi and Pahartali. This research will aid in measuring the AFM1 content in raw milk and helping to address public health issues.

The black cumin (Nigella sativa) seed oil has a long history of use for food ingredients. The goal of this study is to figure out the nutritional and physicochemical properties of two indigenous black cumin cultivars (locally known as "Kali Jira" and "Kalonji") seed and oil. Additionally, the effects of extraction time and particle size on oil yield generation was investigated. The values of moisture content, crude protein content, crude fat, crude fiber, total ash, and carbohydrate of Kali Jira seed were 5.29%, 19.83%, 41.3%, 18.96%, 5.10%, and 9.52% whereas Kalonji had 5.43%, 19.95%, 40.3%, 14.28%, 4.35%, and 14.69%, respectively. In the Kali Jira and Kalonji types, the mineral analysis revealed significant potassium levels (829.11 mg/100 g, 746.27 mg/100 g), followed by phosphorus, calcium, sodium, iron and zinc. The minimum oil yield was 46.77% in the Kalonji variety compared to Kali Jira, which had 49.57% after the extraction time of 2 hrs for 1.4-2.5 mm particle size. On the other hand, a maximum oil yield of 91.08% and 94.77% was found in Kalonji seed and Kali Jira seed, respectively, following a 6-hour extraction time for 0.25-0.5 mm particle size. Characterization of extracted seed oil (iodine value, saponification value, and acid value) indicated the abundance of unsaturation in oil. FTIR analysis was used to investigate functional groups. According to the findings of this study, black cumin and extracted oil are rich in nutrients and can be used to treat lifestyle problems.

Dahi is a fermented milk product containing probiotic lactic acid bacteria. This study aimed to isolate, identify, and characterize lactic acid-producing bacteria from native Dahi and evaluate their viability in orange juice using natural prebiotics. Dahi samples were obtained from local shops in Chattogram and Bogura, Bangladesh. Lactic acid-producing bacteria were isolated using MRS (de Mann Rogosa and Sharpe) medium. The isolated bacteria were identified through colony morphology, biochemical tests, and probiotic characteristics. Molecular identification was performed using polymerase chain reaction (PCR) targeting conserved 16S rDNA regions. Isolates of the genus Lactobacillus and Lactococcus lactis sp. Lactis were confirmed and used to develop probiotic orange juice. Prebiotics (Asparagus falcatus and Zingiber officinale) were added to the juice to support probiotic growth. The inoculated cell’s viability and the juice’s physicochemical parameters were evaluated during fermentation (48 hours) and storage (28 days). All fruit juice samples showed a mean number of viable cells of at least 1×105 CFU/mL during the 48-hour fermentation and 28-day storage in the refrigerator. Using natural prebiotics positively affected the survival of lactic acid bacteria, as demonstrated by bacterial colony growth on Petri dishes. Developing probiotic fruit juice enriched with prebiotics could be an effective alternative for individuals allergic or intolerant to milk-based products. Incorporating lactic acid bacteria from native Dahi into orange juice, combined with natural prebiotics, resulted in viable probiotic cells throughout fermentation and storage.