The forest community structure and dynamic structure provide basic data for in-depth study of the community maintenance mechanisms and succession processes of urban forest ecosystems. Moreover, the study on the dynamics of suburban forest communities can provide a reference for the succession process of forest communities under severe human disturbance. In this context, this study analyzes the species composition and community structure of a 6 ha subtropical secondary evergreen broad-leaved forest plot in Wuchaoshan National Forest Park, Hangzhou, Eastern China. There are 36,757 existing woody plants (with DBH ≥ 1 cm), belonging to 94 species (45 evergreen species, and 48 deciduous species) in 63 genera of 34 families, with a plant density of 6126 plants/ha. The overall size–class distribution shows an obvious inverted “J” shape. Species with the top-five important values in the sample are Schima superba, Camellia fraternal, Symplocos anomala, Cyclobalanopsis glauca, Eurya rubiginosa, accounting for 57.3% of all individuals within the sample. In addition, different species have different habitat preferences and different distribution areas. The Wuchaoshan forest is in the middle and late stages of the succession. Different interference histories in the community succession process are demonstrated through studies of secondary evergreen broad-leaved forests. Long-term community monitoring contributes to indicating the direction and potential of the secondary forest succession.

Leaf color mutants serve as excellent models for investigating the metabolic pathways involved in chlorophyll biosynthesis, chloroplast development, and photosynthesis in plants. This study aimed to elucidate the mechanisms underlying color formation in the yellow–green leaf mutant (YL) of Magnolia sinostellata by employing physiological, cytological and transcriptomic analyses to compare the mutant with control plants (wild type Magnolia sinostellata, WT). Physiological assessments revealed a reduction in chlorophyll content, particularly chlorophyll b, alongside an increase in the flavonoid level in YL relative to WT. Cytological examinations indicated the presence of defective chloroplasts within the mesophyll cells of the mutants. Transcriptomic analysis identified 8205 differentially expressed genes, with 4159 upregulated and 4046 downregulated. Genes associated with chlorophyll metabolism, flavonoid metabolism, photosynthesis, and signaling pathways were found to play crucial roles in leaf yellowing. In conclusion, this study delineated the phenotypic, physiological, cytological, and transcriptomic differences between YL and WT leaves, offering novel insights into the mechanisms driving leaf yellowing in Magnolia sinostellata.

Paper Mulberry (Broussonetia papyrifera) possesses medicinal, economic, and ecological significance and is extensively used for feed production, papermaking, and ecological restoration due to its ease of propagation, rapid growth rate, and strong stress resistance. The recent completion of the sequencing of the Paper Mulberry genome has prompted further research into the genetic breeding and molecular biology of this important species. A highly stable reference gene is essential to enhance the quantitative analysis of functional genes in Paper Mulberry; however, none has been identified. Accordingly, in this study, the leaves, stems, roots, petioles, young fruits, and mature fruits of Paper Mulberry plants were selected as experimental materials, and nine candidate reference genes, namely, α-TUB1, α-TUB2, β-TUB, H2A, ACT, DnaJ, UBQ, CDC2, and TIP41, were identified by RT-qPCR. Their stability was assessed using the geNorm, Normfinder, Delta Ct, BestKeeper, and RefFinder algorithms, identifying ACT and UBQ as showing the greatest stability. The expression of BpMYB090, which regulates the production of trichomes, was examined in the leaves of plants of the wild type (which have more trichomes) and mutant (which have fewer trichomes) at various developmental stages to validate the results of this study. As a result, their identification addresses a critical gap in the field of Paper Mulberry research, providing a solid foundation for future research that will concentrate on the characterization of pertinent functional genes in this economically valuable species.

Liquidambar formosana Hance is renowned for its rich leaf color and possesses notable advantages, such as robust adaptability, strong resistance to diseases and pests, and rapid growth, making it a preferred choice for urban greening and carbon sequestration forest initiatives. The completion of whole-genome sequencing of L. formosana has spurred an increased interest in exploring the molecular mechanisms underlying seasonal changes in leaf color, marking a significant focus in L. formosana breeding research. However, there is currently a lack of stable reference genes suitable for analyzing the expression patterns of functional genes in L. formosana exhibiting varying leaf colors. This study selected five L. formosana varieties with significant differences in leaf colors. Through the RT-qPCR analysis, and evaluation using BestKeeper, geNorm, NormFinder, Delta Ct, and RefFinder, the expression stability of 14 candidate reference genes was examined. Consequently, two reference genes (LifEF1-α and LifACT) with stable expression, suitable for RT-qPCR of L. formosana with diverse leaf colors, were identified. The stability of these selected reference genes was further validated by examining the LifbHLH137 gene, which promoted the biosynthesis of anthocyanins. This advancement facilitated molecular biology and genetic breeding investigations of L. formosana, providing essential data for the precise quantification of functional genes associated with leaf color variation.

In this work, human serum albumin (HSA) was used to prepare core‐shell taxanes‐loaded nanoparticle (NP) by self‐assembly method. Various experimental technologies were used to character two different taxanes‐loaded HSA NPs and analyse the structure of them. The obtained NPs had a spherical morphology and core‐shell structure. The data revealed that one paclitaxel (PTX)‐loaded HSA NP (∼40 nm) consisted of six HSA molecules and 34 PTX molecules, and one docetaxel (DOC)‐loaded HSA NP (∼30 nm) consisted of five HSA molecules and 40 DOC molecules. The infrared, three‐dimensional fluorescence and ultraviolet absorption spectra showed that intermolecular hydrogen bond occurred between taxanes and polypeptide chain of HSA and resulted in change of its secondary structure. The fluorescence spectra experiment results suggested that PTX and DOC interacted with tryptophan (Trp)‐214, which lie on site I of HSA, and the type of interactions were hydrogen bond and hydrophobic interaction, respectively. It suggested that the binding mode of taxanes with Trp‐214 is the primary factor that determined the size and loading capacity of taxanes‐loaded HSA NPs. This work indicates that such HSA NP is very promising in the field of nanoscale drug delivery systems research.