NASA’s Dragonfly mission will send a rotorcraft lander to the surface of Titan in the mid-2030s. Dragonfly's science themes include investigation of Titan’s prebiotic chemistry, habitability, and potential chemical biosignatures from both water-based “life as we know it” (as might occur in the interior mantle ocean, potential cryovolcanic flows, and/or impact melt deposits) and potential “life, but not as we know it” that might use liquid hydrocarbons as a solvent (within Titan’s lakes, seas, and/or aquifers). Consideration of both of these solvents simultaneously led to our initial landing site in Titan’s equatorial dunes and interdunes to sample organic sediments and water ice, respectively. Ultimately, Dragonfly's traverse target is the 80 km diameter Selk Crater, at 7° N, where we seek previously liquid water that has mixed with surface organics. Our science goals include determining how far prebiotic chemistry has progressed on Titan and what molecules and elements might be available for such chemistry. We will also determine the role of Titan’s tropical deserts in the global methane cycle. We will investigate the processes and processing rates that modify Titan’s surface geology and constrain how and where organics and liquid water can mix on and within Titan. Importantly, we will search for chemical biosignatures indicative of past or extant biological processes. As such, Dragonfly, along with Perseverance, is the first NASA mission to explicitly incorporate the search for signs of life into its mission goals since the Viking landers in 1976.

The factors contributing to the initial selection of a dune site near the Selk impact structure on Titan as the first landing site for the Dragonfly mission are described. These include arrival geometry and aerodynamic/aerothermodynamic considerations, illumination, and Earth visibility, as well as the likely presence of exposed deposits of water-rich material, potentially including materials where molten ice has interacted with organics. Cassini observations of Selk are summarized and interpreted: near-infrared reflectance and microwave emission data indicate water-rich materials in and around the crater. Radar topography data shows the rim of Selk to have slopes on multi-km scales reaching only ∼2° degrees, an order of magnitude shallower than early photoclinometric estimates.

Organic macromolecules (“complex tholins”) were synthesized from a 0.95 N₂ / 0.05 CH₄ atmosphere in a high-voltage AC flow discharge reactor. When placed in liquid water, specific water soluble compounds in the macromolecules demonstrated Arrhenius type first order kinetics between 273 and 313 K and produced oxygenated organic species with activation energies in the range of ∼60 ± 10 kJ mol⁻¹. These reactions displayed half lives between 0.3 and 17 days at 273 K. Oxygen incorporation into such materials—a necessary step toward the formation of biological molecules—is therefore fast compared to processes that occur on geologic timescales, which include the freezing of impact melt pools and possible cryovolcanic sites on Saturn's organic-rich moon Titan. Astrobiology 8, 273–287.

New observations of Titan by the Cassini spacecraft suggest the presence of cryovolcanism on the surface. Cryovolcanism has important astrobiological implications, as it provides a means of exposing Titan's organics to liquid water, transforming hydrocarbons and nitriles into more evolved and oxidized prebiotic species. One possible cryovolcano – the 180 km structure Ganesa Macula – resembles the pancake domes seen on Venus by the Magellan spacecraft. To assess the potential of Ganesa Macula for prebiotic chemistry, we estimate its height using radarclinometry and other methods, and calculate the freezing timescale assuming an initially completely liquid dome. Given height constraints of ~200 m to 4 km, we find that liquid water or water–ammonia environments could be sustained in Ganesa Macula for timescales of the order of 10²–10⁵ years. These timescales open a window for prebiotic chemistry far wider than can be explored in terrestrial laboratory experiments.

Titan has an organic-rich atmosphere and surface with a subsurface liquid water ocean that may represent a habitable environment. In this work, we determined the amount of organic material that can be delivered from Titan's surface to its ocean through impact cratering. We assumed that Titan's craters produce impact melt deposits composed of liquid water that can founder in its lower-density ice crust and estimated the amount of organic molecules that could be incorporated into these melt lenses. We used known yields for HCN and Titan haze hydrolysis to determine the amount of glycine produced in the melt lenses and found a range of possible flux rates of glycine from the surface to the subsurface ocean. These ranged from 0 to 10 ¹¹ mol/Gyr for HCN hydrolysis and from 0 to 10 ¹⁴ mol/Gyr for haze hydrolysis. These fluxes suggest an upper limit for biomass productivity of ∼10 ³ kgC/year from a glycine fermentation metabolism. This upper limit is significantly less than recent estimates of the hypothetical biomass production supported by Enceladus's subsurface ocean. Unless biologically available compounds can be sourced from Titan's interior, or be delivered from the surface by other mechanisms, our calculations suggest that even the most organic-rich ocean world in the Solar System may not be able to support a large biosphere.