Aphids pose a significant threat to global agricultural crop production, leading to widespread pesticide use and resistance. This necessitates the use of alternative substances, like plant secondary metabolites (PSMs). Plants have developed protective compounds known as alkaloids, terpenoids, phenolics, sulfur- and nitrogen-containing metabolites. These compounds exhibit promising characteristics against aphids, such as antifeedant, aphicidal, and disrupting survival fitness. This review highlights the importance and application of secondary metabolites in combating aphid populations. Different insect-resistant substances have different mechanisms for managing aphids and other pests, including defensive signaling, inhibiting growth, and attracting natural predators by releasing herbivore-induced volatiles (HIPV). The application of plant secondary metabolites as biopesticides has proven to be an effective, economical, and eco-friendly alternative to synthetic pesticide chemicals. Furthermore, this review comprehensively discusses the principle role of plant secondary metabolites, encouraging sustainable agricultural practices and emphasizing the integrated management of the aphid population.

Mosquitoes, as disease vectors causing global morbidity and mortality through diseases like malaria, dengue, and Zika, necessitate mosquito population control methods. This study investigated the efficacy of nano-formulated insecticide-based sugar baits in controlling Anopheles gambiae populations and assessed their potential non-target impact on Coccinella septempunctata. This laboratory-based study employed thiolated polymer-coated attractive toxic sugar bait (ATSB) nano-formulations, delivering pesticides via nano-carriers. Adult and larvae populations of insects were collected from rice and cotton fields subjected to bioassays with 0.5% and 1% concentrations of each nano-formulated and conventional insecticide within ATSB solution, alongside a control 100% attractive sugar bait (ASB). Mosquitoes interacted overnight with insecticide-treated baits, and mortality was assessed. Further observations up to 72 h were conducted for potential delayed toxic effects. Results highlighted nano-ATSB carbosulfan’s effectiveness, particularly among organophosphates and pyrethroids. Among pyrethroids, nano-ATSB cypermethrin exhibited high efficacy, while Deltamethrin displayed lower mortality. Among organophosphates, nano-ATSB chlorpyrifos induced substantial mortality. The nano-formulations of insecticide were harmless against C. septempunctata compared to their conventional form. Nano-formulations demonstrated enhanced mortality rates and prolonged efficacy against mosquitoes, having a benign impact on non-target beetles. We expect these results to aid in developing effective plant protection products suitable for IPM practices.

Postharvest losses of fruits and vegetables due to physiological decay, microbial spoilage, and environmental stressors remain a significant challenge in the food supply chain. This review critically examines the synergistic role of essential oil (EO) nanoemulsions and biopolymer-based edible coatings in preserving the quality, safety, and shelf life of perishable produce during cold storage. Nanoemulsions enhance the stability, solubility, and controlled release of bioactive EO compounds (e.g., thymol, carvacrol, cinnamaldehyde), overcoming limitations such as volatility and poor water solubility. Meanwhile, biopolymers like chitosan, alginate, starch, and pectin provide a biodegradable, semi-permeable matrix that mitigates moisture loss, gas exchange, and oxidative damage. Combined, these systems exhibit superior antimicrobial, antioxidant, and barrier properties, effectively delaying ripening, inhibiting pathogen growth (e.g., Botrytis cinerea, E. coli), and retaining nutritional attributes (e.g., vitamin C, phenolic content). Case studies highlight the efficacy of coatings such as chitosan-thyme nanoemulsions for strawberries and alginate-oregano films for apples, demonstrating extended shelf life by 7–15 days under refrigeration. Challenges, including optimisation of EO concentration, sensory compatibility, and industrial scalability, are also discussed. By integrating nanotechnology with green chemistry, biopolymer-EO nanoemulsion coatings represent a sustainable, consumer-friendly alternative to synthetic preservatives, aligning with global demands for reducing food waste and enhancing postharvest resilience.

The water lily aphid, Rhopalosiphum nymphaeae, is the only known aphid that can live in both terrestrial and aquatic conditions. In this study, the complete mitochondrial genome of R. nymphaeae was generated using Illumina sequencing technology. The typical circular DNA mitochondrial genome of R. nymphaeae is 15,772 bp in length, with a high A+T content (84.34%). It contains 37 coding genes (13 protein-coding genes, 22 transport RNAs, and two ribosomal RNAs) and two non-coding regions (one control region and one repeat region). Enc-plot, PR2-bias, and neutrality plot analysis indicated that the codon usage of the protein-coding genes is mainly affected by natural selection. The evolution rate analysis (the ratio of nonsynonymous to synonymous, Ka/Ks) indicated that all the PCGs in R. nymphaeae are under a strong purifying selection. The control region has conserved structure elements, and two types of tandem repeat units exist. The length and sequence of the aphid-unique repeat region has high similarity with closely related species. Phylogenetic analyses determined by both maximum likelihood and Bayesian inference support the monophyly of Aphidinae, Aphidini, Aphidina, and Rhopalosiphina. However, the monophyly of the genera in Rhopalosiphina, such as Rhopalosiphum, is still not resolved. This study may help us to understand the phylogenetic relationship of aphids, and much more aphid data are needed in future studies.

Pollution coming from plastic polymers, particularly polyethylene (PE), poses a serious threat to both humans and animals. The biodegradation of plastics facilitated by insects is a crucial and eco-friendly approach that can be employed to combat this global concern. Recently, the larvae of the greater wax moth Galleria mellonella (L.) have been recognized as avid ‘plastivores’. The current study was aimed at evaluating the feeding efficiency of G. mellonella larvae on PEs of various densities with a co-diet supplementation of wheat germ + honey and beeswax. The results reveal that maximum PE consumption (9.98 ± 1.25 mg) was recorded in the case of 1.0 mm thick PE after a 24 h interval; however, the same scenario also achieved the greatest reduction in larval weight (27.79 ± 2.02 mg). A significant reduction in PE mass (5.87 ± 1.44 mg) was also observed in 1.0 mm PE when fed beeswax; however, the larvae experienced minimal weight loss (9.59 ± 3.81 mg). The larvae exhibited a higher PE consumption in 1.0 mm PE, indicating that the lower the density of PE, the greater the consumed area. Moreover, the biodegradation levels were notably higher within the 24 h interval. In conclusion, these findings suggest that the density of PEs and the supplementation of the co-diet have an impact on PE biodegradation. Additionally, the utilization of G. mellonella for the biodegradation of PE proves effective when combined with beeswax, resulting in minimal weight loss of the larvae. Our findings offer initial insights into how Galleria mellonella larvae biodegrade polyethylene (PE) of four different densities, along with co-diet supplementation. This approach helps us evaluate how varying densities affect degradation rates and provides a better understanding of the larvae’s capabilities. Additionally, our observations at three specific time intervals (24, 48, and 72 h) allow us to identify the time required for achieving degradation rates. Through examining these time points, our method offers valuable insights into the initial phases of plastic consumption and biodegradation.