Drought stress disrupts the molecular-level water balance in plants, and severe water deficiency can be fatal for cotton plants. However, mild water deficits or short-term drought stress may enhance crop resilience, increasing yields. The present study aims to determine the optimal watering time and irrigation amount to induce drought tolerance in cotton seedlings during drought training. Specifically, the investigation focuses on identifying the ideal day for watering and the corresponding irrigation volume that effectively triggers the transition of cotton plants into a state of enhanced resistance to drought stress during the seedling stage. In this study, the CSM-CROPGRO-Cotton model was utilized, and our objectives were to (i) evaluate the predictive capability of CSM-CROPGRO-Cotton for yield estimation in field experiments in Xinjiang and (ii) simulate and assess the range of time during the seedling stage when cotton plants can withstand drought stress without reducing yields, identifying irrigation strategies that induce drought training while maintaining yield under mild water deficiency. The model was validated using yield data from field experiments conducted in 2023. The validation criteria included a normalized root mean square error $\left(nRMSE\right)>10\%$ and a coefficient of determination $\left(r^2\right)>85\%$ for yield; for the leaf area index (LAI), the criterion was $\left(r^2\right)>90\%$, with a degree of agreement of $\left(d\right)>75\%$. The results demonstrated the accuracy of the CSM-CROPGRO-Cotton model in predicting cotton yield. Based on the validated CSM-CROPGRO-Cotton model, this study employed the LINUX crop model batch-processing technique to efficiently simulate 357 different irrigation strategies by adjusting the amount of “first irrigation” and timing. The findings revealed that in the irrigation scheme for cotton during the seedling stage, when the amount of first irrigation was in the lower range of 10 mm to 15 mm, the cotton plants underwent drought training during the early growth stage, and their yields did not exhibit drastic fluctuations due to reduced amounts of first irrigation. The suitable period for first irrigation for drought training was from 25 June to 6 July, and the amount of first irrigation could save approximately 57.14% in irrigation water. This implies that subjecting cotton plants to a certain level of drought training can enhance their stress tolerance and increase yields. This finding holds great significance for cotton cultivation in drought-prone regions.

To explore the effects of different water-saving treatments on rice plant growth and select suitable water-saving irrigation strategies for aerobic rice varieties, we conducted relevant field experiments from June to October 2023 at Jiangsu Runguo Agricultural Development Co., Ltd., China, which is located in a north subtropical monsoon climate where the soil is alkaline sandy loam. Four water treatments were set up, including the control of local conventional irrigation (CK, without water stress), mild water-saving treatment (W1, 20% more water saved than CK), moderate water-saving treatment (W2, 30% more water saved than CK), and severe water-saving treatment (W3, 40% more water saved than CK). The experiment results showed that rice plant heights were inhibited and leaf chlorophyll contents increased under all water-saving treatments compared to CK. Among them, the MDA content in paddy leaves under the W1 treatment decreased, while the activities of SOD and POD were enhanced and the membrane lipid peroxidation capacity of rice was also enhanced. Meanwhile, the results showed that the rice yield and quality under the W1 treatment significantly improved. Based on those experiments, a comprehensive evaluation of rice plant height, chlorophyll content, grain yield, yield components, and rice quality was conducted using the TOPSIS entropy weight method. It was preliminarily concluded that the suitable irrigation scheme for south and central Jiangsu was 20% water-saving irrigation compared with CK. In summary, under the premise of maintaining the economic yield of rice cultivation, an appropriate water irrigation plan helped save water resources and promote rice growth.

With the changing global climate, drought stress will pose a considerable challenge to the sustainable development of agriculture in arid regions. The objective of this study was to explore the resistance and water demand of cotton plants to water stress during the flowering and boll setting stage. The experimental plot was in Huaxing Farm of Changji city. The plots were irrigated, respectively, at 100% (as the control), 90%, 85% and 80% of the general irrigation amount in the local area. The relationship between the various measured indexes and final yield under different deficit irrigation (DI) treatments was studied. The results showed that deficit irrigation impacted the growth and development processes of cotton during the flowering and boll setting stage. There was a high negative correlation (R² > 0.95) between the maximum leaf area index and yield. Similarly, there was a high correlation between malondialdehyde content and yield. Meanwhile, 90% of the local cotton irrigation contributed to water saving and even increasing cotton yield. Furthermore, based on the results, the study made an initial optimization to the local irrigation scheme by utilizing the DSSAT model. It was found that changing the irrigation interval to 12 days during the stage could further enhance cotton yield and conserve resources.

Wheat plants are impacted by Fusarium head blight (FHB) infection, which poses a huge threat to wheat growth, development, storage and food safety. In this study, a fungal strain was isolated from diseased wheat plants and identified as Fusarium asiaticum F1, known to be a member of the Fusarium graminearum species complex, agents causally responsible for FHB. In order to control this disease, new alternatives need to be developed for the use of antagonistic bacteria. Bacillus velezensis E2 (B. velezensis E2), isolated from a previous investigation in our laboratory, showed a notable inhibitory effect on F. asiaticum F1 growth and deoxynivalenol (DON) synthesis in grains. The spore germination of F. asiaticum F1 was significantly reduced and the spores showed vesicular structures when treated with B. velezensis E2. Observations using scanning electron microscopy (SEM) showed that the hyphae of F. asiaticum F1 were shrunken and broken when treated with B. velezensis E2. The RNA-seq results of F1 hyphae treated with B. velezensis E2 showed that differentially expressed genes (DEGs), which were involved in multiple metabolic pathways such as toxin synthesis, autophagy process and glycan synthesis, especially the genes associated with DON synthesis, were significantly downregulated. In summary, those results showed that B. velezensis E2 could inhibit F. asiaticum F1 growth and reduce the gene expression of DON synthesis caused by F1. This study provides new insights and antagonistic mechanisms for the biological control of FHB during wheat growth, development and storage.

Investigating the physiological responses and resistance mechanisms in plants under drought stress provides critical insights for optimizing irrigation water utilization efficiency and promoting the development of irrigation science. In this study, cotton seedlings were cultivated in a light incubator. Three drought stress levels were applied: mild (M1, 50–55% field moisture), moderate (M2, 45–50%), and severe (M3, 40–45%). Transcriptome analysis was performed under mild and severe stress. The results revealed that differentially expressed genes (DEGs) related to proline degradation were down-regulated and proline content increased in cotton. Under different stress treatments, cotton exhibited a stress-intensity-dependent regulation of carbohydrate metabolism and soluble sugar content decreased and then increased. And the malondialdehyde content analysis revealed a dose-dependent relationship between stress intensity and membrane lipid peroxidation. Stress activated the antioxidant system, leading to the down-regulation of DEGs for reactive oxygen species production in the mitogen-activated protein kinase (MAPK) signaling pathway. Concurrently, superoxide dismutase activity and peroxidase content increased to mitigate oxidative damage. Meanwhile, the photosynthetic performance of cotton seedlings was inhibited. Chlorophyll content, stomatal conductance, the net photosynthetic rate, the transpiration rate and water use efficiency were significantly reduced; intercellular carbon dioxide concentration and leaf stomatal limitation value increased. But photosynthesis genes (e.g., PSBO (oxygen-evolving enhancer protein 1), RBCS (ribulose bisphosphate carboxylase small chain), and FBA2 (fructose-bisphosphate aldolase 1)) in cotton were up-regulated to coordinate the photosynthetic process. Furthermore, cotton seedlings differentially regulated key biosynthesis and signaling components of phytohormonal pathways including abscisic acid, indoleacetic acid and gibberellin. This study elucidates the significant gene expression of drought-responsive transcriptional networks and relevant physiological response in cotton seedlings and offers a theoretical basis for developing water-saving irrigation strategies.

Screening of Bacillus with antagonistic effects on paddy mold pathogens to provide strain resources for biological control of mold in Oryza sativa L. screening of Bacillus isolates antagonistic towards Aspergillus tubingensis from rhizosphere soil of healthy paddy; classification and identification of antagonistic strains by biological characteristics and 16S rDNA sequence analysis; transcriptome sequencing after RNA extraction from Bacillus-treated Aspergillus tubingensis; and extraction of inhibitory crude proteins of Bacillus by ammonium sulfate precipitation; inhibitory crude protein and Bacillus spp. were treated separately for A. tubingensis and observed by scanning electron microscopy (SEM). An antagonistic strain of Bacillus, named B7, was identified as Paenibacillus polymyxa by 16S rDNA identification and phylogenetic evolutionary tree comparison analysis. Analysis of the transcriptome results showed that genes related to secondary metabolite biosynthesis such as antifungal protein were significantly downregulated. SEM results showed that the mycelium of A. tubingensis underwent severe rupture after treatment with P. polymyxa and antifungal proteins, respectively. In addition, the sporocarp changed less after treatment with P. polymyxa, and the sporangium stalks had obvious folds. P. polymyxa B7 has a good antagonistic effect against A. tubingensis and has potential for biocontrol applications of paddy mold pathogens.