This research aimed to understand the use of air gliding arc (GA) plasma to generate plasma-activated water (PAW) for fixing nitrogen in water and the chemical properties of PAW on the germination of rice seeds. The N₂, NO, and OH molecules in GA plasma led to NO₃⁻, NO₂⁻, and H₂O₂ formation in the PAW. The NO₃⁻, NO₂⁻, and H₂O₂ contents in PAW rapidly decreased after 5 days of storage. The experiment was arranged in a completely randomised design using GA plasma discharged above the surface of deionised (DI) water with different airflow rates (2, 3, 4, 5, and 6 L/min) compared to the control (DI water). The NO₃⁻ and NO₂⁻ contents increased, resulting in an increase in total nitrogen (N) and gibberellic acid (GA₃) accumulation in rice seeds. The PAW at an airflow rate of 5 L/min was optimal for enhancing radicle emergence at 48 and 72 h, germination, germination index, shoot length, fresh weight, and dry weight of seedlings. Therefore, air GA plasma to generate PAW is an efficient method for producing nitrogen in a soluble form, which can support the germination processes and early growth of rice seedlings.