Background/Objectives: Antibiotic resistance is a growing global health threat, requiring comprehensive investigations into the occurrence, distribution, and diversity of antibiotics across environmental systems. This study aimed to examine the distribution and prevalence of commonly used antibiotics in various wastewater sources across the United Arab Emirates (UAE), providing insights into potential environmental reservoirs contributing to antimicrobial resistance dissemination. Methods: Wastewater samples were collected from the outlets of three hospitals, three residential communities, and the influent and return activated sludge of two wastewater treatment plants. Samples were prepared using solid-phase extraction (SPE) and analyzed via liquid chromatography–mass spectrometry (LC–MS/MS) to quantify antibiotic concentrations and compare their distribution among sources. Results: Significant variations were observed in antibiotic concentrations across the different wastewater sources. Ciprofloxacin concentrations were highest in hospital wastewater, reaching up to 247.9 ng/mL, while cefuroxime and vancomycin ranged between 0.2 and 10.9 ng/mL across samples. Clindamycin was notably prevalent in wastewater treatment plant samples (up to 10.9 ng/mL), whereas vancomycin and cefuroxime were dominant in residential community samples, reflecting diverse antibiotic usage patterns and persistence in treatment systems. Conclusions: The findings reveal substantial antibiotic contamination in United Arab Emirates wastewater systems, underscoring the need for ongoing surveillance and regulatory measures. Continuous monitoring, coupled with effective wastewater treatment and antibiotic stewardship strategies, is critical to mitigating the environmental spread of antimicrobial resistance and safeguarding public and environmental health.

The indiscriminate use of antibiotics in agriculture has raised concerns about antibiotic residues in food products, necessitating robust analytical methods for detection and quantification. In this study, our primary aim was to develop a robust and advanced liquid chromatography-tandem mass spectrometry (LC-MS/MS) methodology specifically designed for the accurate quantification of ticarcillin degradation products in tomato leaves. The choice of ticarcillin as the target analyte stems from its frequent use in agriculture and the potential formation of degradation products, which can pose a threat to food safety. The use of tomatoes as the target sample matrix in this study is justified by their significance in human diets, their widespread cultivation, and their suitability as a model for assessing antibiotic residue dynamics in diverse agricultural environments. By optimizing the MS/MS parameters, the study successfully demonstrates the practicality and reliability of the employed LC-MS/MS method in accurately assessing ticarcillin degradation product (Thiophene-2-Acetic acid and Thiophene-3-Acetic acid) levels. The chromatographic separation was achieved using a specialized column, ensuring high resolution and sensitivity in detecting analytes. Multiple reaction monitoring (MRM) data acquisition was employed to enhance the selectivity and accuracy of the analysis. The developed method exhibited excellent linearity and precision, meeting the stringent requirements for antibiotic residue analysis in complex matrices. Key outcomes of this study include the successful identification and quantification of ticarcillin and its degradation products in tomato leaves, providing crucial insights into the fate of this antibiotic in agricultural settings. The methodology’s applicability was further demonstrated by analyzing real-world samples, highlighting its potential for routine monitoring and ensuring food safety compliance. In summary, our study constitutes a noteworthy advancement in the domain of antibiotic residue analysis, offering a reliable method for quantifying ticarcillin degradation products in tomato leaves. The optimized parameters and MRM-based LC-MS/MS approach enhance the precision and sensitivity of the analysis, opening up opportunities for further studies in the assessment of antibiotic residues in agricultural ecosystems.