Correction Note:Article KILIÇKAYA SELVI, E., GÜLER, N., GÜVEN, S., & MAKBUL, S. (2024). Determination of Chemical Compositions, Antioxidant, DNA Cleavage and Binding Properties of Vincetoxicum Tmoleum Extract. Studia Universitatis Babeș-Bolyai Chemia, 69(1), 163–174, https://doi.org/10.24193/subbchem.2024.1.10 has been corrected in the Platform (Volume 69, No. 1, 2024 | Studia Universitatis Babeș-Bolyai Chemia).Date: Wednesday, September 10, 2025 10:40 AMSubject: Request for Article Correction - Request from Emine Kılıçkaya Selvi.The changes include:-References 34 and 43 was revised.-Figure 1 and Table 1 was revised. Other parts of the article were left untouched. No changes were made that would compromise the integrity and content of the article. Editors’ Note: The article was corrected on 10.09.2025. The pdf file of the volume also includes a Corrigendum. In the present study, phenolic compounds, antioxidant activities, DNA cleavage and binding effects of Vincetoxicum tmoleum (Vt) collected from west of Anatolia (Manisa city) were investigated. Antioxidant potentials of the extracts were characterized with their total phenolic and flavonoid contents, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), and FRAP (ferric reducing antioxidant power assay) tests. Besides, the DNA cleavage and binding features of V. tmoleum extract were studied using pBR322 DNA and CT-DNA, respectively. Phenolic compounds of the extract were analyzed by high performance liquid chromatography (HPLC-DAD). As a result, V. tmoleum methanol extract was found to have the high total phenolic and total flavonoid content and antioxidant effect. Strong positive correlations were also found between DPPH and TFC (r = 0.995; p < 0.01), and TPC (r = 0.989;p < 0.01), ABTS (r = 0.994;p < 0.01) and FRAP(r=0,995; p < 0.01). Methanol extract of V. tmoleum had large amounts of p-coumaric acid, vanillic acid and catechin. While V. tmoleum samples showed weak DNA cleavage activity, they showed DNA binding activity at 50 µM concentration, that is, the potential to be an intercalation agent in this concentration.

Vincetoxicum members offer important qualities in both conventional and modern medicine. In the present study, V. scandens from the northeast of Turkey (Trabzon city) was investigated for its phenolic compounds, antioxidant activities, DNA cleavage and binding effects. Phenolic compounds of the V. scandens methanol extract were analysed by high performance liquid chromatography (HPLC-DAD), while their total phenolic and total flavonoid contents were determined spectrophotometrically. The antioxidant potential of the extract was characterised using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2?-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), and FRAP (ferric reducing antioxidant power assay) tests. In addition, the DNA cleavage and binding features of the V. scandens extract were studied using pBR322 DNA and CTDNA, respectively. The results indicated that the V. scandens methanol extract contained a large amount of catechin, as well as a high total phenolic and total flavonoid content. On the other hand, the DPPH and ABTS antioxidant tests revealed that V. scandens did not exhibit high antioxidant activity. The methanol extract of the studied plant was also rich in catechin, p-coumaric acid and vanillic acid. While the V. scandens methanol extract exhibited weak antioxidant and DNA cleavage activity, it showed DNA binding activity at 50 ?M, thus demonstrating the potential to be an intercalation agent in this concentration. This study provides the first report on the total phenolic content, total flavonoid content, DNA cleavage and DNA binding activity as well as antioxidant activity of V. scandens.

Bovine rotaviruses (BRVs) are significant causative agents of severe diarrhea in newborn calves, resulting in substantial economic losses in the livestock industry. Inhibition of bovine rotavirus using extracts prepared from a Cucurbitaceae plant, which contains trypsin protease inhibitors, might offer a potential anti-rotaviral effect in vitro. Ecballium elaterium (E. elaterium) belongs to the Cucurbitaceae family, indigenous to the Mediterranean, contains E. elaterium trypsin isoinhibitors (EETIso), and has been used in traditional medicine. This study aimed to evaluate the in vitro efficacy of E. elaterium extract against bovine rotavirus infections. Ethanol extracts were prepared from E. elaterium seeds and fruit juice, and their non-toxic concentrations were determined using MA-104 cells. The cells were infected with bovine rotavirus in the presence of E. elaterium extract. The results demonstrated a significant decrease in the rotavirus titer in vitro upon treatment with the E. elaterium extract, suggesting its potential as a therapeutic agent against bovine rotavirus-induced diarrhea in calves. The utilization of E. elaterium extract may contribute to reduced calf mortality, lower medication costs, and improved economic value in cattle farming.

Background: Apart from the areas where natural dyes are used a lot, if staining studies on tissues increase, their use in pathology laboratory will become widespread. Two basic elements in cell tissues, the nucleus, and cytoplasm, should be stained and evaluated together for histopathological diagnosis. The most commonly used dye combinations for this diagnosis are Hematoxylin-Eosin dyes, also known as nuclei and cytoplasm dyes. Histological staining studies of natural origin did not reveal staining studies on the same tissue with the extracts of two plants. Methods and Results: In this study, roselle (Hibiscus sabdariffa L.) and red cabbage (Brassica oleracea) leaves were used as a source of natural dye and also liver and kidney tissues of Wistar albino rat as dye biomaterials. The nuclei and cytoplasm regions of rat kidney and liver tissues were stained in different colors with these plants and the use of metal salts. Relying upon hematoxylin-eosin should it ever be scarce will result in the scarcity of plant-based dyes on the market.Conclusion: Since a more colorful, different nucleus and cytoplasm staining was obtained compared to previous work with the same tissues. It is expected that the introduction of new natural dye alternatives where histological dye production is not widespread will contribute to the world’s economy and industry.

In this study, Xanthoria parietina samples were collected from different regions of Türkiye like Yozgat (Xp3), Izmit (Xp14), and Kütahya (Xp20). Anthracenedione, anthraquinone (parietin) contents of the lichens were determined quantitatively by GC-MS and spectrophotometric methods. The interaction of lichen extracts with pBR322 DNA and CT-DNA was examined by performing an agarose gel electrophoresis method. The cell proliferative activities of Xanthoria parietina samples were tested against the colon cancer cell line (DLD-1) by MTT assay. As a results of the GC-MS and spectrophotometric analysis, the highest and the lowest parietin contents were found for Xp20 and Xp14 extracts, respectively. These results were supported by those of the DNA cleavage, binding, and toxicity studies. The Xp14 sample can be considered as a drug that could be a new approach to cancer treatment, as it has the lowest polyaromatic hydrocarbon content and is not toxic for the cell.

This study aimed to evaluate the anticancer and antibacterial drug status of biomass, including zinc oxide (ZnO) nanoparticles (NPs) synthesized from two plant extracts according to the results of Topoisomerase-I enzyme inhibition, DNA cleavage, DNA binding, and antibacterial activity tests. The Malus domestica (apple) and Tilia cordata (linden) plants were dried and powdered, and their extracts were prepared in pure water. X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Fourier Transform Infrared (FTIR) analyses were applied for the characterization of the light-yellow powder ZnO NPs. Additionally, Topoisomerase-I enzyme inhibition, DNA cleavage, and DNA binding analyses were performed by agarose gel electrophoresis method. Powder ZnO NPs (biomass) were analyzed by the agar well diffusion method by using two gram-positive and two gram-negative bacteria. According to the XRD analysis results, the peaks obtained show that there is no foreign matter or secondary diffraction peak other than the hexagonal ZnO structure. It was observed that ZnO NPs from Tilia cordata extract are effective against Klebsiella pneumoniae. ZnO NPs from Malus domestica extract have the potential to be an effective human Topoisomerase-I enzyme inhibitor. There was poor DNA cleavage activity in all samples. In ZnO NPs from linden extract, there was an interaction at a 1mg/mL concentration of CT-DNA.