Chromatographic separation ofn-hexane and ethyl acetate fraction of a crude methanol extract obtained from aerial parts of theScorzonera aucherianaDC collected from Sivas province of Turkey yielded nine natural compounds; two new 3-caffeoyl-quinic acid analogs (1-2), one new taraxasterol oleate (3), and six known triterpenoids taraxasterol (4), taraxasterol acetate (5), ptiloepoxide (6), lupeol (7), lupeol acetate (8), andβ-sitosterol (9) were characterized. The structures of the isolated compounds were elucidated on the basis of NMR (1H,13C, COSY, HMBC, HSQC, and TOCSY), UV, FT-IR and LC-Q-TOF-MS spectrometric data.

Scorzonera species exhibit various biological activities largely dependent on their chemical composition. While numerous studies have investigated these species’ secondary volatile metabolite content, to our knowledge, this is the first comprehensive study focused on Scorzonera pseudolanata. The present study aimed to identify and analyze the secondary volatile metabolites in different parts of S. pseudolanata. The composition of these metabolites was determined using gas chromatography–mass spectrometry (GC-MS). The resulting data were further analyzed through biplot analysis to differentiate among the plant parts. A total of 46 secondary volatile compounds were identified across all examined tissues. Hexadecane (13.6%) was the dominant compound in the roots, phytone (16.36%) in the leaves, and nonadecane (56.45%) in the seeds. The secondary volatile metabolite profile of S. pseudolanata differs markedly from that of other Scorzonera species, a distinction effectively visualized using a biplot diagram. This study represents the first detailed investigation into the secondary volatile metabolite composition of S. pseudolanata. It offers foundational data that may inform future in-depth research, thereby contributing to a broader understanding of the phytochemistry of this species.