A glucose biosensor was layer-by-layer assembled on a modified glassy carbon electrode (GCE) from a nanocomposite of NAD(P)⁺-dependent glucose dehydrogenase, aminated polyethylene glycol (mPEG), carboxylic acid-functionalized multi-wall carbon nanotubes (fMWCNTs), and ionic liquid (IL) composite functional polymers. The electrochemical electrode was denoted as NF/IL/GDH/mPEG-fMWCNTs/GCE. The composite polymer membranes were characterized by cyclic voltammetry, ultraviolet-visible spectrophotometry, electrochemical impedance spectroscopy, scanning electron microscopy, and transmission electron microscopy. The cyclic voltammogram of the modified electrode had a pair of well-defined quasi-reversible redox peaks with a formal potential of −61 mV (vs. Ag/AgCl) at a scan rate of 0.05 V s⁻¹. The heterogeneous electron transfer constant (k_s) of GDH on the composite functional polymer-modified GCE was 6.5 s⁻¹. The biosensor could sensitively recognize and detect glucose linearly from 0.8 to 100 µM with a detection limit down to 0.46 μM (S/N = 3) and a sensitivity of 29.1 nA μM⁻¹. The apparent Michaelis–Menten constant (${\mathrm{K}}_{\mathrm{m}}^{\mathrm{app}}$) of the modified electrode was 0.21 mM. The constructed electrochemical sensor was compared with the high-performance liquid chromatography method for the determination of glucose in commercially available glucose injections. The results demonstrated that the sensor was highly accurate and could be used for the rapid and quantitative determination of glucose concentration.

Renewable energy consumption is considered as the main form of energy consumption in the future. The carbon emissions produced by renewable energy can be approximately ignored, and renewable energy is essential for regional sustainable development. In this study, we used the Durbin model with panel data to explore the spatial dependence between renewable energy consumption the and carbon emissions of China’s 30 provinces from 1997 to 2017. The results show that: (1) there is a negative spatial correlation between renewable energy consumption and carbon emissions, and “High-Low” areas are mainly concentrated in southern provinces in 1997–2011; (2) the center of gravity of renewable energy consumption moves southwest, which is consistent with the center of gravity of carbon emissions; (3) renewable energy consumption has a significant inhibitory effect on carbon emissions of a local region, but the spatial spillover effect is not significant. Specifically, a 1% increase in renewable energy consumption in a region will reduce carbon emissions by 0.05%. Finally, on the basis of this study, it was proposed to give full play to the advantages of renewable energy in the western region, and further accelerate the development of the renewable energy industry.