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Jie Zhang
Lanzhou Veterinary Research Institute

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Short Biography

Jie Zhang, Ph.D. (born in 1971) is a professor at the Lanzhou Veterinary Research Institute (LVRI), China. She leads the FMD Diagnosis Research Group and the FMD Prevent and Control Innovation Team. Jie received her Bachelor’s and Master’s degrees in Clinical Medicine and her PhD in Microbiology from the Institute of Applied Ecology, Chinese Academy of Sciences (CAS). Her recent research activities include the development of rapid diagnostic methods, subunit vaccine and virus-like particle vaccine. Currently, her work on research themes such as the development of virus-like particle vaccine against FMD SAT2 virus and generating PRRSV as live but replication-incompetent virus vaccine is under experiment.

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Preprint content
Published: 07 June 2021
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Background: Foot and mount disease (FMD) is a highly contagious, economically and politically significant transboundary animal disease which specifically affects all cloven-hoofed animals; cattle, pig, goat, sheep and many wild artiodactyls. Five of the seven (O, A, C, SAT1, SAT2, SAT3 and Asia 1) serotypes of FMD virus (O, A, C, SAT1, SAT2) are endemic in Ethiopia; however, limited information on the current FMDV status and the circulating serotype is available in the country. Therefore, this study was conduct to isolate and molecularly identify the FMD viruses using a panel of virological detection assays. Methods: An outbreak-based cross-sectional study was conducted in Addis Ababa and Bishoftu during 2013 and 2014 to isolate and to molecularly identify the circulating serotype of FMDV. A total of 20 samples were collected from clinically infected cattle and pigs during the outbreak and virus isolation and molecular serotype identification was carried out at the National Veterinary Institute (NVI), Bishoftu Ethiopia. Cells were monitored for cytopathic effects (CPE) daily and frozen when CPE were developed. Serotyping of FMD viruses were made by applying classical PCR from cell cultures showing CPE.Results: All tested samples showed cytopathic effect (CPE) on BHK-21 cell culture and serotype O was identified using PCR. The DNA bands were visualized to the expected sizes.Conclusions: Based on these result, a continuous understanding of the molecular epidemiology of the disease along with the proper vaccination matching for the circulating serotype; O is critical for implementation effective control and prevention programs eventually for the eradication of the disease.

ACS Style

Bisratgebriel Tesfaye Muchie; Ashenafi Kiros Wubshet; Aklilu Feleke Haile; Hayelom Michael Deyo; Kazimierz Tarasiuk; Zygmunt Pejsak; Zhang Jie. Isolation and Molecular Identification of Foot and Mouth Disease Virus Circulating Around Central Ethiopia. 2021, 1 .

AMA Style

Bisratgebriel Tesfaye Muchie, Ashenafi Kiros Wubshet, Aklilu Feleke Haile, Hayelom Michael Deyo, Kazimierz Tarasiuk, Zygmunt Pejsak, Zhang Jie. Isolation and Molecular Identification of Foot and Mouth Disease Virus Circulating Around Central Ethiopia. . 2021; ():1.

Chicago/Turabian Style

Bisratgebriel Tesfaye Muchie; Ashenafi Kiros Wubshet; Aklilu Feleke Haile; Hayelom Michael Deyo; Kazimierz Tarasiuk; Zygmunt Pejsak; Zhang Jie. 2021. "Isolation and Molecular Identification of Foot and Mouth Disease Virus Circulating Around Central Ethiopia." , no. : 1.

Journal article
Published: 27 May 2021 in Viruses
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An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SAT2 are poorly understood. Similarly, the particular roles of the three structural proteins in novel vaccine design and development remain unexplained. We therefore constructed VP1, VP0, and VP3 encoding gene (SAT2:JX014256 strain) separately fused with His-SUMO (histidine-small ubiquitin-related modifier) inserted into pET-32a cassette to express the three recombinant proteins and separately evaluated their antigenicity and immunogenicity in mice. The fusion protein was successfully expressed and purified by the Ni-NTA resin chromatography. The level of serum antibody, spleen lymphocyte proliferation, and cytokines against the three distinct recombinant proteins were analyzed. Results showed that the anti-FMDV humoral response was triggered by these proteins, and the fusion proteins did enhance the splenocyte immune response in the separately immunized mice. We observed low variations among the three fusion proteins in terms of the antibody and cytokine production in mice. Hence, in this study, results demonstrated that the structural proteins of SAT2 FMDV could be used for the development of immunodiagnostic kits and subunit vaccine designs.

ACS Style

Guoxiu Li; Ashenafi Wubshet; Yaozhong Ding; Qian Li; Junfei Dai; Yang Wang; Qian Hou; Jiao Chen; Bing Ma; Anna Szczotka-Bochniarz; Susan Szathmary; Yongguang Zhang; Jie Zhang. Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus. Viruses 2021, 13, 1005 .

AMA Style

Guoxiu Li, Ashenafi Wubshet, Yaozhong Ding, Qian Li, Junfei Dai, Yang Wang, Qian Hou, Jiao Chen, Bing Ma, Anna Szczotka-Bochniarz, Susan Szathmary, Yongguang Zhang, Jie Zhang. Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus. Viruses. 2021; 13 (6):1005.

Chicago/Turabian Style

Guoxiu Li; Ashenafi Wubshet; Yaozhong Ding; Qian Li; Junfei Dai; Yang Wang; Qian Hou; Jiao Chen; Bing Ma; Anna Szczotka-Bochniarz; Susan Szathmary; Yongguang Zhang; Jie Zhang. 2021. "Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus." Viruses 13, no. 6: 1005.

Microbiology
Published: 23 April 2021 in Frontiers in Microbiology
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African swine fever (ASF) has caused huge economic losses to the swine industry worldwide. Since there is no commercial ASF vaccine available, an early diagnosis is extremely important to prevent and control the disease. In this study, ASF virus (ASFV) capsid protein-encoding gene (p72) was selected and used to design primers for establishing a one-step visual loop-mediated isothermal amplification (LAMP) assay with neutral red, a pH-sensitive dye, as the color shift indicator. Neutral red exhibited a sharp contrast of color change from faint orange (negative) to pink (positive) during LAMP for detection of ASFV. The designed primer set targeting highly conserved region of the p72 gene was highly specific to ASFV and showed no cross-reactivity with other swine viruses. The detection limit for the one-step visual LAMP developed was 10 copies/reaction based on the recombinant plasmid containing the p72 gene of ASFV. More importantly, the developed one-step visual LAMP showed high consistency with the results of the real-time polymerase chain reaction (qPCR) method recommended by World Organization for Animal Health (OIE). Furthermore, the results demonstrate that the colorimetric detection with this LAMP assay could be directly applied for the whole blood and serum samples without requiring genome extraction. Based on our results, the developed one-step visual LAMP assay is a promising penside diagnostic tool for development of early and cost-effective ASF monitoring program that would greatly contribute to the prevention and control of ASF.

ACS Style

Yang Wang; Junfei Dai; Yongsheng Liu; Jifei Yang; Qian Hou; Yunwen Ou; Yaozhong Ding; Bing Ma; Haotai Chen; Miaomiao Li; Yuefeng Sun; Haixue Zheng; Keshan Zhang; Ashenafi Kiros Wubshet; Alexei D. Zaberezhny; Taras I. Aliper; Kazimierz Tarasiuk; Zygmunt Pejsak; Zhijie Liu; Yongguang Zhang; Jie Zhang. Development of a Potential Penside Colorimetric LAMP Assay Using Neutral Red for Detection of African Swine Fever Virus. Frontiers in Microbiology 2021, 12, 1 .

AMA Style

Yang Wang, Junfei Dai, Yongsheng Liu, Jifei Yang, Qian Hou, Yunwen Ou, Yaozhong Ding, Bing Ma, Haotai Chen, Miaomiao Li, Yuefeng Sun, Haixue Zheng, Keshan Zhang, Ashenafi Kiros Wubshet, Alexei D. Zaberezhny, Taras I. Aliper, Kazimierz Tarasiuk, Zygmunt Pejsak, Zhijie Liu, Yongguang Zhang, Jie Zhang. Development of a Potential Penside Colorimetric LAMP Assay Using Neutral Red for Detection of African Swine Fever Virus. Frontiers in Microbiology. 2021; 12 ():1.

Chicago/Turabian Style

Yang Wang; Junfei Dai; Yongsheng Liu; Jifei Yang; Qian Hou; Yunwen Ou; Yaozhong Ding; Bing Ma; Haotai Chen; Miaomiao Li; Yuefeng Sun; Haixue Zheng; Keshan Zhang; Ashenafi Kiros Wubshet; Alexei D. Zaberezhny; Taras I. Aliper; Kazimierz Tarasiuk; Zygmunt Pejsak; Zhijie Liu; Yongguang Zhang; Jie Zhang. 2021. "Development of a Potential Penside Colorimetric LAMP Assay Using Neutral Red for Detection of African Swine Fever Virus." Frontiers in Microbiology 12, no. : 1.

Review
Published: 18 November 2019 in Viruses
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Foot and mouth disease (FMD) endemicity in Ethiopia’s livestock remains an ongoing cause for economic concern, with new topotypes still arising even in previously unaffected areas. FMD outbreaks occur every year almost throughout the country. Understanding the outbreak dynamics, endemic serotypes, and lineage profiles of FMD in this country is very critical in designing control and prevention programs. For this, detailed information on outbreak dynamics in Ethiopia needs to be understood clearly. In this article, therefore, we review the spatial and temporal patterns and dynamics of FMD outbreaks from 2008 to 2018. The circulating serotypes and the topotypic profiles of the virus are also discussed. FMD outbreak data were obtained from; reports of MoARD (Ministry of Agriculture and Rural Development)/MoLF (Ministry of livestock and Fishery, NVI (National Veterinary Institute), and NAHDIC (National Animal Health Diagnostic and Investigation Center); published articles; MSc works; PhD theses; and documents from international organizations. To effectively control and prevent FMD outbreaks, animal health agencies should focus on building surveillance systems that can quickly identify and control ongoing outbreaks and implement efficient preventive measures.

ACS Style

Ashenafi Kiros Wubshet; Junfei Dai; Qian Li; Jie Zhang. Review on Outbreak Dynamics, the Endemic Serotypes, and Diversified Topotypic Profiles of Foot and Mouth Disease Virus Isolates in Ethiopia from 2008 to 2018. Viruses 2019, 11, 1076 .

AMA Style

Ashenafi Kiros Wubshet, Junfei Dai, Qian Li, Jie Zhang. Review on Outbreak Dynamics, the Endemic Serotypes, and Diversified Topotypic Profiles of Foot and Mouth Disease Virus Isolates in Ethiopia from 2008 to 2018. Viruses. 2019; 11 (11):1076.

Chicago/Turabian Style

Ashenafi Kiros Wubshet; Junfei Dai; Qian Li; Jie Zhang. 2019. "Review on Outbreak Dynamics, the Endemic Serotypes, and Diversified Topotypic Profiles of Foot and Mouth Disease Virus Isolates in Ethiopia from 2008 to 2018." Viruses 11, no. 11: 1076.

Journal article
Published: 01 December 2018 in Journal of Veterinary Research
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Introduction: The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information. Material and Methods: A FMDV SAT-D8 one-step RT-PCR was established based on the 1D2A2B genes of the SAT serotype viruses with a multiplex primer set. FMDV A, O, C, and Asia 1 serotypes, other vesicular disease viruses, inactivated SAT viruses, and 125 bovine, ovine, caprine and porcine tissue samples collected from the Chinese mainland were included for evaluating the assay. Results: The new RT-PCR was proven to be specific without cross-reactions with Eurasian FMDV, swine vesicular disease virus (SVDV), Seneca valley virus (SVV), or other common viral pathogens of cattle, sheep, goat, and pig. An around 257 bp-sized amplicon clearly appeared when the inactivated SAT viruses were detected. However, all 125 samples collected from FMDV-susceptible animals from the Chinese mainland which has not known SAT epidemics showed negative results. Conclusions: A FMDV SAT-D8 one-step RT-PCR is a promising method for primary screening for FMDV SAT serotypes.

ACS Style

Ya-Li Liu; Yao-Zhong Ding; Jun-Fei Dai; Bing Ma; Ji-Jun He; Wei-Min Ma; Jian-Liang Lv; Xiao-Yuan Ma; Yun-Wen Ou; Jun Wang; Yong-Sheng Liu; Hui-Yun Chang; Yong-Lu Wang; Qiang Zhang; Xiang-Tao Liu; Yong-Guang Zhang; Jie Zhang. Development of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses. Journal of Veterinary Research 2018, 62, 431 -437.

AMA Style

Ya-Li Liu, Yao-Zhong Ding, Jun-Fei Dai, Bing Ma, Ji-Jun He, Wei-Min Ma, Jian-Liang Lv, Xiao-Yuan Ma, Yun-Wen Ou, Jun Wang, Yong-Sheng Liu, Hui-Yun Chang, Yong-Lu Wang, Qiang Zhang, Xiang-Tao Liu, Yong-Guang Zhang, Jie Zhang. Development of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses. Journal of Veterinary Research. 2018; 62 (4):431-437.

Chicago/Turabian Style

Ya-Li Liu; Yao-Zhong Ding; Jun-Fei Dai; Bing Ma; Ji-Jun He; Wei-Min Ma; Jian-Liang Lv; Xiao-Yuan Ma; Yun-Wen Ou; Jun Wang; Yong-Sheng Liu; Hui-Yun Chang; Yong-Lu Wang; Qiang Zhang; Xiang-Tao Liu; Yong-Guang Zhang; Jie Zhang. 2018. "Development of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses." Journal of Veterinary Research 62, no. 4: 431-437.

Journal article
Published: 11 July 2018 in Viruses
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Foot-and-mouth disease (FMD) is a highly contagious disease that results in enormous economic loses worldwide. Although the protection provided by vaccination is limited during early infection, it is recognized as the best method to prevent FMD outbreaks. Furthermore, the mechanism of host early responses against foot-and-mouth disease virus (FMDV) infection remains unclear. In our study, a pig kidney cell line (PK-15) was used as a cell model to reveal the mechanism of early pig responses to FMDV infection. Four non-treated control and four FMDV-treated PK-15 cells were sequenced with RNA-seq technology, and the differentially expressed genes (DEGs) were analyzed. The results showed that 1212 DEGs were in the FMDV-infected PK-15 cells, including 914 up-regulated and 298 down-regulated genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly enriched in the tumor necrosis factor (TNF), cytokine-cytokine receptor interaction, NOD-like receptor, toll-like receptor, NF-κB, and the chemokine signaling pathways. To verify the results of the DEGs, 30 immune-related DEGs (19 up-regulated and 11 down-regulated) were selected for Quantitative Reverse Transcriptase polymerase chain reaction (RT-qPCR) verification. The results showed that RT-qPCR-measured genes exhibited a similar pattern as the RNA-seq analyses. Based on bioinformatics analysis, during FMDV early infection, we found that a series of cytokines, such as interleukins (IL6), chemokines (CXCL2, CCL20 and CCL4), and transcription factors (ZFP36, FOS, NFKBIA, ZBTB3, ZNF503, ZNF283, dymeclin (DYM), and orthodenticle homeobox 1 (OTX1)) were involved in the battle between FMDV and the host. Combined with their features and functions, we propose inflammation as the main early mechanism by which the host responds to FMDV infection. These data provide an additional panel of candidate genes for deciphering the mechanisms of a host’s early response against FMDV infection.

ACS Style

Tianliang Zhang; Haotai Chen; Linlin Qi; Jie Zhang; Run Wu; Yongguang Zhang; Yuefeng Sun. Transcript Profiling Identifies Early Response Genes against FMDV Infection in PK-15 Cells. Viruses 2018, 10, 364 .

AMA Style

Tianliang Zhang, Haotai Chen, Linlin Qi, Jie Zhang, Run Wu, Yongguang Zhang, Yuefeng Sun. Transcript Profiling Identifies Early Response Genes against FMDV Infection in PK-15 Cells. Viruses. 2018; 10 (7):364.

Chicago/Turabian Style

Tianliang Zhang; Haotai Chen; Linlin Qi; Jie Zhang; Run Wu; Yongguang Zhang; Yuefeng Sun. 2018. "Transcript Profiling Identifies Early Response Genes against FMDV Infection in PK-15 Cells." Viruses 10, no. 7: 364.

Review
Published: 28 November 2017 in Virology Journal
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This review summarized the molecular determinants of the acid stability of FMDV in order to explore the uncoating mechanism of FMDV and improve the acid stability of vaccines. The foot-and-mouth disease virus (FMDV) capsid is highly acid labile and tends to dissociate into pentameric subunits at acidic condition to release viral RNA for initiating virus replication. However, the acid stability of virus capsid is greatly required for the maintenance of intact virion during the process of virus culture and vaccine production. The conflict between the acid lability in vivo and acid stability in vitro of FMDV capsid promotes the selection of a series of amino acid substitutions which can confer resistance to acid-induced FMDV inactivation. In order to explore the uncoating activity of FMDV and enhance the acid stability of vaccines, we summarized the available works about the pH stability of FMDV. In this review, we analyzed the intrinsic reasons for the acid instability of FMDV from the structural and functional aspects. We also listed all substitutions obtained by different research methods and showed them in the partial capsid of FMDV. We found that a quadrangle region in the viral capsid was the place where a great many pH-sensitive residues were distributed. As the uncoating event of FMDV is dependent on the pH-sensitive amino acid residues in the capsid, this most pH-sensitive position indicates a potential candidate location for RNA delivery triggered by the acid-induced coat disassociation. This review provided an overview of the pH stability of FMDV. The study of pH stability of FMDV not only contributes to the exploration of molecule and mechanism information for FMDV uncoating, but also enlightens the development of FMDV vaccines, including the traditionally inactivated vaccines and the new VLP (virus-like particle) vaccines.

ACS Style

Fu Yuanfang; Pinghua Li; Xueqing Ma; Zengjun Lu; Pu Sun; Xingwen Bai; Jing Zhang; Huifang Bao; Yimei Cao; Dong Li; Yuanfang Fu; Yingli Chen; QiFeng Bai; Jie Zhang; Zaixin Liu. The pH stability of foot-and-mouth disease virus. Virology Journal 2017, 14, 233 -233.

AMA Style

Fu Yuanfang, Pinghua Li, Xueqing Ma, Zengjun Lu, Pu Sun, Xingwen Bai, Jing Zhang, Huifang Bao, Yimei Cao, Dong Li, Yuanfang Fu, Yingli Chen, QiFeng Bai, Jie Zhang, Zaixin Liu. The pH stability of foot-and-mouth disease virus. Virology Journal. 2017; 14 (1):233-233.

Chicago/Turabian Style

Fu Yuanfang; Pinghua Li; Xueqing Ma; Zengjun Lu; Pu Sun; Xingwen Bai; Jing Zhang; Huifang Bao; Yimei Cao; Dong Li; Yuanfang Fu; Yingli Chen; QiFeng Bai; Jie Zhang; Zaixin Liu. 2017. "The pH stability of foot-and-mouth disease virus." Virology Journal 14, no. 1: 233-233.

Review
Published: 01 April 2016 in Genomics
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Long noncoding (lnc)RNAs comprise a diverse group of transcripts including large intervening noncoding (linc)RNAs, natural antisense transcripts (NATs) and intronic lncRNAs. The functions and mechanisms of more than 200 lncRNAs have been studied in vitro and the results suggest that lncRNAs may be molecular markers of prognosis in cancer patients. Some lncRNAs can promote virus replication and allow escape from cytosolic surveillance to suppress antiviral immunity. For example, lncRNA can cause persistent infection by Theiler's virus, and microRNA (miR)-27a/b is important for efficient murine cytomegalovirus (MCMV) replication. The available evidence suggests that lncRNAs may be potential targets of novel antiviral drugs.

ACS Style

Yao-Zhong Ding; Zhong-Wang Zhang; Ya-Li Liu; Chong-Xu Shi; Jie Zhang; Yong-Guang Zhang. Relationship of long noncoding RNA and viruses. Genomics 2016, 107, 150 -154.

AMA Style

Yao-Zhong Ding, Zhong-Wang Zhang, Ya-Li Liu, Chong-Xu Shi, Jie Zhang, Yong-Guang Zhang. Relationship of long noncoding RNA and viruses. Genomics. 2016; 107 (4):150-154.

Chicago/Turabian Style

Yao-Zhong Ding; Zhong-Wang Zhang; Ya-Li Liu; Chong-Xu Shi; Jie Zhang; Yong-Guang Zhang. 2016. "Relationship of long noncoding RNA and viruses." Genomics 107, no. 4: 150-154.

Research article
Published: 03 August 2014 in BioMed Research International
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The information about the crystal structure of porcine reproductive and respiratory syndrome virus (PRRSV) leader protease nsp1αis available to analyze the roles of tRNA abundance of pigs and codon usage of thensp1 αgene in the formation of this protease. The effects of tRNA abundance of the pigs and the synonymous codon usage and the context-dependent codon bias (CDCB) of thensp1 αon shaping the specific folding units (α-helix,β-strand, and the coil) in the nsp1αwere analyzed based on the structural information about this protease from protein data bank (PDB: 3IFU) and thensp1 αof the 191 PRRSV strains. By mapping the overall tRNA abundance along thensp1 α, we found that there is no link between the fluctuation of the overall tRNA abundance and the specific folding units in the nsp1α, and the low translation speed of ribosome caused by the tRNA abundance exists in thensp1 α. The strong correlation between some synonymous codon usage and the specific folding units in the nsp1αwas found, and the phenomenon of CDCB exists in the specific folding units of the nsp1α. These findings provide an insight into the roles of the synonymous codon usage and CDCB in the formation of PRRSV nsp1αstructure.

ACS Style

Yao-Zhong Ding; Ya-Nan You; Dong-Jie Sun; Hao-Tai Chen; Yong-Lu Wang; Hui-Yun Chang; Li Pan; Yu-Zhen Fang; Zhong-Wang Zhang; Peng Zhou; Jian-Liang Lv; Xin-Sheng Liu; Jun-Jun Shao; Fu-Rong Zhao; Tong Lin; Laszlo Stipkovits; Zygmunt Pejsak; Yong-Guang Zhang; Jie Zhang. The Effects of the Context-Dependent Codon Usage Bias on the Structure of the nsp1αof Porcine Reproductive and Respiratory Syndrome Virus. BioMed Research International 2014, 2014, 1 -10.

AMA Style

Yao-Zhong Ding, Ya-Nan You, Dong-Jie Sun, Hao-Tai Chen, Yong-Lu Wang, Hui-Yun Chang, Li Pan, Yu-Zhen Fang, Zhong-Wang Zhang, Peng Zhou, Jian-Liang Lv, Xin-Sheng Liu, Jun-Jun Shao, Fu-Rong Zhao, Tong Lin, Laszlo Stipkovits, Zygmunt Pejsak, Yong-Guang Zhang, Jie Zhang. The Effects of the Context-Dependent Codon Usage Bias on the Structure of the nsp1αof Porcine Reproductive and Respiratory Syndrome Virus. BioMed Research International. 2014; 2014 ():1-10.

Chicago/Turabian Style

Yao-Zhong Ding; Ya-Nan You; Dong-Jie Sun; Hao-Tai Chen; Yong-Lu Wang; Hui-Yun Chang; Li Pan; Yu-Zhen Fang; Zhong-Wang Zhang; Peng Zhou; Jian-Liang Lv; Xin-Sheng Liu; Jun-Jun Shao; Fu-Rong Zhao; Tong Lin; Laszlo Stipkovits; Zygmunt Pejsak; Yong-Guang Zhang; Jie Zhang. 2014. "The Effects of the Context-Dependent Codon Usage Bias on the Structure of the nsp1αof Porcine Reproductive and Respiratory Syndrome Virus." BioMed Research International 2014, no. : 1-10.

Research article
Published: 10 February 2014 in BioMed Research International
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RNA interference (RNAi) has been proved to be a powerful tool for foot-and-mouth disease virus FMDV inhibition in vitro and in vivo. We established five stable baby hamster kidney 21 cell lines (BHK-21) containing five short hairpin RNAs (shRNAs) expression plasmids (p3D1shRNA, p3D2shRNA, p3D3shRNA, p3D4shRNA, and p3D5shRNA) targeting 3D gene of FMDV. Immunofluorescent assay, virus titration, and real-time quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) were conducted to detect the effect of shRNAs on FMDV replication. After challenged with FMDV of O/CHA/99, two cell lines (p3D1shRNA and p3D4shRNA) showed a significant reduction in the synthesis of viral protein and RNA, accompanied by a sharp decrease in viral yield, and the inhibition could last for at least thirty passages. We developed an efficient procedure for the establishment and evaluation of stable cell lines for anti-FMDV research based on RNAi technology, which can be a candidate method for anti-FMDV research.

ACS Style

Yuan-Xing Gu; Zong-Liang Gao; Jian-Hua Zhou; Jie Zhang; Yong-Sheng Liu. Establishment and Evaluation of Stable Cell Lines Inhibiting Foot-and-Mouth Disease Virus by RNA Interference. BioMed Research International 2014, 2014, 1 -7.

AMA Style

Yuan-Xing Gu, Zong-Liang Gao, Jian-Hua Zhou, Jie Zhang, Yong-Sheng Liu. Establishment and Evaluation of Stable Cell Lines Inhibiting Foot-and-Mouth Disease Virus by RNA Interference. BioMed Research International. 2014; 2014 ():1-7.

Chicago/Turabian Style

Yuan-Xing Gu; Zong-Liang Gao; Jian-Hua Zhou; Jie Zhang; Yong-Sheng Liu. 2014. "Establishment and Evaluation of Stable Cell Lines Inhibiting Foot-and-Mouth Disease Virus by RNA Interference." BioMed Research International 2014, no. : 1-7.

Journal article
Published: 01 January 2014 in Journal of Veterinary Science
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A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription- PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.

ACS Style

Yao-Zhong Ding; Jian-Hua Zhou; Li-Na Ma; Yan-Ni Qi; Gang Wei; Jie Zhang; Yong-Guang Zhang. A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C. Journal of Veterinary Science 2014, 15, 423 -426.

AMA Style

Yao-Zhong Ding, Jian-Hua Zhou, Li-Na Ma, Yan-Ni Qi, Gang Wei, Jie Zhang, Yong-Guang Zhang. A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C. Journal of Veterinary Science. 2014; 15 (3):423-426.

Chicago/Turabian Style

Yao-Zhong Ding; Jian-Hua Zhou; Li-Na Ma; Yan-Ni Qi; Gang Wei; Jie Zhang; Yong-Guang Zhang. 2014. "A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C." Journal of Veterinary Science 15, no. 3: 423-426.