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Ashenafi Kiros Wubshet is a dedicated veterinarian and microbiologist. Currently a PhD fellow at the Lanzhou Veterinary Research Institute (LVRI), Chinese Academy of Agricultural Sciences (CAAS), Gansu province, Lanzhou City, PR China. He is doing his research in novel chimeric vaccine development against FMD SAT2 serotype with the Foot-and-Mouth Disease Diagnosis Research small group, under the FMD disease Prevent and Control Innovation Team. He was born in 1986 in Northern Ethiopia. Ashenafi received his Doctor of Veterinary Medicine and Master’s degree in Veterinary Microbiology from Haramaya and Addis Ababa University, Ethiopia. He has published seven scientific articles as a first author in peer-reviewed journals. He has also co-authored for a total of 11 interesting scientific review and research articles.
Background: Foot and mount disease (FMD) is a highly contagious, economically and politically significant transboundary animal disease which specifically affects all cloven-hoofed animals; cattle, pig, goat, sheep and many wild artiodactyls. Five of the seven (O, A, C, SAT1, SAT2, SAT3 and Asia 1) serotypes of FMD virus (O, A, C, SAT1, SAT2) are endemic in Ethiopia; however, limited information on the current FMDV status and the circulating serotype is available in the country. Therefore, this study was conduct to isolate and molecularly identify the FMD viruses using a panel of virological detection assays. Methods: An outbreak-based cross-sectional study was conducted in Addis Ababa and Bishoftu during 2013 and 2014 to isolate and to molecularly identify the circulating serotype of FMDV. A total of 20 samples were collected from clinically infected cattle and pigs during the outbreak and virus isolation and molecular serotype identification was carried out at the National Veterinary Institute (NVI), Bishoftu Ethiopia. Cells were monitored for cytopathic effects (CPE) daily and frozen when CPE were developed. Serotyping of FMD viruses were made by applying classical PCR from cell cultures showing CPE.Results: All tested samples showed cytopathic effect (CPE) on BHK-21 cell culture and serotype O was identified using PCR. The DNA bands were visualized to the expected sizes.Conclusions: Based on these result, a continuous understanding of the molecular epidemiology of the disease along with the proper vaccination matching for the circulating serotype; O is critical for implementation effective control and prevention programs eventually for the eradication of the disease.
Bisratgebriel Tesfaye Muchie; Ashenafi Kiros Wubshet; Aklilu Feleke Haile; Hayelom Michael Deyo; Kazimierz Tarasiuk; Zygmunt Pejsak; Zhang Jie. Isolation and Molecular Identification of Foot and Mouth Disease Virus Circulating Around Central Ethiopia. 2021, 1 .
AMA StyleBisratgebriel Tesfaye Muchie, Ashenafi Kiros Wubshet, Aklilu Feleke Haile, Hayelom Michael Deyo, Kazimierz Tarasiuk, Zygmunt Pejsak, Zhang Jie. Isolation and Molecular Identification of Foot and Mouth Disease Virus Circulating Around Central Ethiopia. . 2021; ():1.
Chicago/Turabian StyleBisratgebriel Tesfaye Muchie; Ashenafi Kiros Wubshet; Aklilu Feleke Haile; Hayelom Michael Deyo; Kazimierz Tarasiuk; Zygmunt Pejsak; Zhang Jie. 2021. "Isolation and Molecular Identification of Foot and Mouth Disease Virus Circulating Around Central Ethiopia." , no. : 1.
An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SAT2 are poorly understood. Similarly, the particular roles of the three structural proteins in novel vaccine design and development remain unexplained. We therefore constructed VP1, VP0, and VP3 encoding gene (SAT2:JX014256 strain) separately fused with His-SUMO (histidine-small ubiquitin-related modifier) inserted into pET-32a cassette to express the three recombinant proteins and separately evaluated their antigenicity and immunogenicity in mice. The fusion protein was successfully expressed and purified by the Ni-NTA resin chromatography. The level of serum antibody, spleen lymphocyte proliferation, and cytokines against the three distinct recombinant proteins were analyzed. Results showed that the anti-FMDV humoral response was triggered by these proteins, and the fusion proteins did enhance the splenocyte immune response in the separately immunized mice. We observed low variations among the three fusion proteins in terms of the antibody and cytokine production in mice. Hence, in this study, results demonstrated that the structural proteins of SAT2 FMDV could be used for the development of immunodiagnostic kits and subunit vaccine designs.
Guoxiu Li; Ashenafi Wubshet; Yaozhong Ding; Qian Li; Junfei Dai; Yang Wang; Qian Hou; Jiao Chen; Bing Ma; Anna Szczotka-Bochniarz; Susan Szathmary; Yongguang Zhang; Jie Zhang. Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus. Viruses 2021, 13, 1005 .
AMA StyleGuoxiu Li, Ashenafi Wubshet, Yaozhong Ding, Qian Li, Junfei Dai, Yang Wang, Qian Hou, Jiao Chen, Bing Ma, Anna Szczotka-Bochniarz, Susan Szathmary, Yongguang Zhang, Jie Zhang. Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus. Viruses. 2021; 13 (6):1005.
Chicago/Turabian StyleGuoxiu Li; Ashenafi Wubshet; Yaozhong Ding; Qian Li; Junfei Dai; Yang Wang; Qian Hou; Jiao Chen; Bing Ma; Anna Szczotka-Bochniarz; Susan Szathmary; Yongguang Zhang; Jie Zhang. 2021. "Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus." Viruses 13, no. 6: 1005.
Foot and mouth disease (FMD) endemicity in Ethiopia’s livestock remains an ongoing cause for economic concern, with new topotypes still arising even in previously unaffected areas. FMD outbreaks occur every year almost throughout the country. Understanding the outbreak dynamics, endemic serotypes, and lineage profiles of FMD in this country is very critical in designing control and prevention programs. For this, detailed information on outbreak dynamics in Ethiopia needs to be understood clearly. In this article, therefore, we review the spatial and temporal patterns and dynamics of FMD outbreaks from 2008 to 2018. The circulating serotypes and the topotypic profiles of the virus are also discussed. FMD outbreak data were obtained from; reports of MoARD (Ministry of Agriculture and Rural Development)/MoLF (Ministry of livestock and Fishery, NVI (National Veterinary Institute), and NAHDIC (National Animal Health Diagnostic and Investigation Center); published articles; MSc works; PhD theses; and documents from international organizations. To effectively control and prevent FMD outbreaks, animal health agencies should focus on building surveillance systems that can quickly identify and control ongoing outbreaks and implement efficient preventive measures.
Ashenafi Kiros Wubshet; Junfei Dai; Qian Li; Jie Zhang. Review on Outbreak Dynamics, the Endemic Serotypes, and Diversified Topotypic Profiles of Foot and Mouth Disease Virus Isolates in Ethiopia from 2008 to 2018. Viruses 2019, 11, 1076 .
AMA StyleAshenafi Kiros Wubshet, Junfei Dai, Qian Li, Jie Zhang. Review on Outbreak Dynamics, the Endemic Serotypes, and Diversified Topotypic Profiles of Foot and Mouth Disease Virus Isolates in Ethiopia from 2008 to 2018. Viruses. 2019; 11 (11):1076.
Chicago/Turabian StyleAshenafi Kiros Wubshet; Junfei Dai; Qian Li; Jie Zhang. 2019. "Review on Outbreak Dynamics, the Endemic Serotypes, and Diversified Topotypic Profiles of Foot and Mouth Disease Virus Isolates in Ethiopia from 2008 to 2018." Viruses 11, no. 11: 1076.
Ashenafi Wubshet. Incidence of Heifer Mastitis and Identification of Major Associated Pathogens in Dairy Farms at Wolaita Soddo Town, Southern Ethiopia. Journal of Dairy, Veterinary & Animal Research 2017, 5, 1 -9.
AMA StyleAshenafi Wubshet. Incidence of Heifer Mastitis and Identification of Major Associated Pathogens in Dairy Farms at Wolaita Soddo Town, Southern Ethiopia. Journal of Dairy, Veterinary & Animal Research. 2017; 5 (5):1-9.
Chicago/Turabian StyleAshenafi Wubshet. 2017. "Incidence of Heifer Mastitis and Identification of Major Associated Pathogens in Dairy Farms at Wolaita Soddo Town, Southern Ethiopia." Journal of Dairy, Veterinary & Animal Research 5, no. 5: 1-9.
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Wakuma Mitku; Wolaita Sodo University School of Veterinary Medicine; Wale Tesfaye; Ashenafi Wubshet; Ashenafi Kiros Wubshet. Review on Stem Cell Therapy and their Role in Cancer Treatment. International Journal of Biotechnology and Bioengineering 2017, 3, 71 -79.
AMA StyleWakuma Mitku, Wolaita Sodo University School of Veterinary Medicine, Wale Tesfaye, Ashenafi Wubshet, Ashenafi Kiros Wubshet. Review on Stem Cell Therapy and their Role in Cancer Treatment. International Journal of Biotechnology and Bioengineering. 2017; 3 (4):71-79.
Chicago/Turabian StyleWakuma Mitku; Wolaita Sodo University School of Veterinary Medicine; Wale Tesfaye; Ashenafi Wubshet; Ashenafi Kiros Wubshet. 2017. "Review on Stem Cell Therapy and their Role in Cancer Treatment." International Journal of Biotechnology and Bioengineering 3, no. 4: 71-79.