Tomatoes are parasitized by a number of pathogens, including Fusarium oxysporum f. sp. lycopersici, causal agent of fusarium wilt. Fresh vegetable fruits can be contaminated with various fungi that produce mycotoxins, which is an important issue for human health. The objective of this paper was to isolate, determine, and identify causal organisms of tomato wilt and fruit rot, based on the pathogens morphological and molecular characteristics. Samples of diseased plants showing symptoms of tomato wilt were collected from different localities in the production region of Vojvodina. Fruits with symptoms of fusarium rot were collected from storage and warehouses. The isolation and morphological determination of the fungus were performed on PDA and Czapek?s nutrient media. Isolates from diseased plants growing in field, designated as TFW1-TFW12 and seven isolates from diseased tomato fruits (TFM1-TFM7) were chosen for further investigation. For identification of the fungal isolates, the polymerase chain reaction (PCR) was also used. The EF1/EF2 primer pair was used for molecular identification of Fusarium sp. Nine analyzed samples were found to contain DNA fragments 700 bp in size.

Seed germination is first critical and the most sensitive stage in the life cycle of plants compromise the seedlings establishment. Salt and drought tolerance testing in initial stages of plant development is of vital importance, because the seed with more rapid germination under salt or water deficit conditions may be expected to achieve a rapid seedling establishment, resulting in higher yields. The aim of this study was to determine whether the pea seed germination and seedling growth were inhibited by the salt toxicity and osmotic effect during the seedling development, and also identification of the sensitive seedling growth parameters in response to those stresses. Based on the obtained results, pea has been presented to be more tolerant to salt than water stress during germination and early embryo growth. Investigated cultivars showed greater susceptibility to both abiotic stresses when it comes growth parameters compared to seed germination.

The aim of this study was to determine the variability of oil and protein content in winter rapeseed cultivars affected by seeding date. Four cultivars (Banaćanka, Slavica, Express, Valeska) were sown at six seeding dates (SD1-21 August, SD2-31 August, SD3-10 September, SD4-21 September, SD5-1 October, SD6-9 October) across two cropping seasons. The trial was arranged as Randomized Complete Block Design (RCBD) with four replications. Analysis of variance (ANOVA) showed that the effect of year, cultivar and seeding date were highly significant for oil and protein content. Increased oil content in the second year (2010/2011) was related to weather conditions which were favorable for rapeseed. However, protein content was significantly higher in the first year (2009/2010). Oil content ranged between 41.19% (Valeska) and 42.69% (Express). Significantly lowest oil content across seeding dates was found in SD6 (40.67%), and highest in SD4 (41.86%) and SD1 (41.61%). Valeska showed significantly highest mean protein content (21.54%). Protein content was highest in SD6 (20.18%). Oil content decreased with delayed seeding. Highly significant negative correlation (r = -0.730) existed between oil and protein content. Seeding date had significant influence on oil and protein content in rapeseed. Cultivar Express and SD4 can be pointed out, based on the achieved oil content, and cultivar Valeska for high protein content. Study results may be helpful in recommending optimal rapeseed seeding date in the region.

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