Grain cereals such as wheat, barley, rice, and maize are the nutritional basis of humans and animals worldwide. Thus, these crop plants are essential in terms of global food security. We conducted a bibliometric assessment of scientific documents and patents related to wheat and barley through the Scopus database. The number of documents published per year, their affiliation and corresponding scientific areas, the publishing journals, document types and languages were metricized. The main keywords included in research publications concerning these crops were also analysed globally and clustered in thematic groups. In the case of keywords related to agronomy or genetics and molecular biology, we considered documents dated up to 1999, and from 2000 to 2018, separately. Comparison of the results obtained for wheat and barley revealed some remarkable different trends, for which the underlying reasons are further discussed.

Agriculture is the basis for food production on a global scale. Sustainable agriculture tries to improve or maintain the quality of food without compromising the environment. As sessile organisms, plants cannot avoid adverse environmental conditions and contact with other living organisms. The damage caused to plants by other living organisms such as parasites and pathogens (virus, bacteria, fungi, nematodes or insects) brings about what is known as biotic stress. Plants are constantly exposed to biotic stress, which causes changes in plant metabolism involving physiological damages that lead to a reduction of their productivity. To fight biotic stress, plants have developed sophisticated defense mechanisms. Thus, understanding plant defense mechanisms might prevent important crop and economic losses. In this article, a bibliometric analysis of biotic stress is carried out. Different aspects of the publications are analyzed, such as publication type, research field, journal type, countries and their institutions, as well as the keyword occurrence frequency, and finally special attention is paid to the plant studied by the leading countries and institutions. As expected, journals selected by authors to publish their relevant findings are plant-specific journals. However, it should be noted that the fourth position, in terms of the number of publications per journal, is occupied by BMC Genomics journal. Such a journal considers mainly articles on genomics, which indicates the involvement of genetic factors in the control of biotic stress. Analysis of the keywords used in publications about biotic stress shows the great interest in the biotic–abiotic stress interaction, in the gene expression regulation in plants as well as phytohormones in the current research. In short, the great effort made by the scientific community in the biotic and abiotic stresses field with the aim to understand, regulate and control plant damages caused by biotic stress agents will help in the development of sustainable agriculture.

FVB/N mice are one of the most common inbred strains for the generation of transgenic animals. This mouse strain is preferred for transgenesis because of its fertilized oocytes, which have unique pronuclei for microinjection, and its vigorous reproductive performance along with consistently large litter sizes. However, these inbred mice carry a retinal degeneration mutation caused by a proviral insertion into the Pdeb gene, encoding the β subunit of cGMP phosphodiesterase. This mutation ( Pdeb^rd1, formerly known as rd) results in postnatal rod photoreceptor degeneration and causes severe visual impairment, which may be relevant for behavioural and vision-related research. This deficit can be overcome by crossing these mice with other mouse strains carrying the wild-type allele at the Pdeb locus. We have devised a simple polymerase chain reaction (PCR)-based method for distinguishing between the mutant and the wild-type alleles, thus allowing the efficient monitoring of the Pdeb^rd1 mutation in FVB/N-derived transgenic mice prior to experimentation where visual deficit is expected to have an influence in the phenotype.

Reproductive development of higher plants comprises successive events of organ differentiation and growth which finally lead to the formation of a mature fruit. However, most of the genetic and molecular mechanisms which coordinate such developmental events are yet to be identified and characterized. Arlequin (Alq), a semi-dominant T-DNA tomato mutant showed developmental changes affecting flower and fruit ripening. Sepals were converted into fleshy organs which ripened as normal fruit organs and fruits displayed altered ripening features. Molecular characterization of the tagged gene demonstrated that it corresponded to the previously reported TOMATO AGAMOUS-LIKE 1 (TAGL1) gene, the tomato ortholog of SHATTERPROOF MADS-box genes of Arabidopsis thaliana, and that the Alq mutation promoted a gain-of-function phenotype caused by the ectopic expression of TAGL1. Ectopic overexpression of TAGL1 resulted in homeotic alterations affecting floral organ identity that were similar to but stronger than those observed in Alq mutant plants. Interestingly, TAGL1 RNAi plants yielded tomato fruits which were unable to ripen. They displayed a yellow-orange color and stiffness appearance which are in accordance with reduced lycopene and ethylene levels, respectively. Moreover, pericarp cells of TAGL1 RNAi fruits showed altered cellular and structural properties which correlated to both decreased expression of genes regulating cell division and lignin biosynthesis. Over-expression of TAGL1 is able to rescue the non-ripening phenotype of rin and nor mutants, which is mediated by the transcriptional activation of several ripening genes. Our results demonstrated that TAGL1 participates in the genetic control of flower and fruit development of tomato plants. Furthermore, gene silencing and over-expression experiments demonstrated that the fruit ripening process requires the regulatory activity of TAGL1. Therefore, TAGL1 could act as a linking factor connecting successive stages of reproductive development, from flower development to fruit maturation, allowing this complex process to be carried out successfully.