Lead contamination in aquatic products is one of the main hazard factors. The aptasensor is a promising detection method for lead ion (Pb(II)) because of its selectivity, but it is easily affected by pH. The combination of ion-imprinted polymers(IIP) with aptamers may improve their stability in different pH conditions. This paper developed a novel electrochemical biosensor for Pb(II) detection by using aptamer-imprinted polymer as a recognition element. The glassy carbon electrode was modified with gold nanoparticles and aptamers. After the aptamer was induced by Pb(II) to form a G-quadruplex conformation, a chitosan-graphene oxide was electrodeposited and cross-linked with glutaraldehyde to form an imprint layer, improving the stability of the biosensor. Under the optimal experimental conditions, the current signal change (∆I) showed a linear correlation of the content of Pb(II) in the range of 0.1–2.0 μg/mL with a detection limit of 0.0796 μg/mL (S/N = 3). The biosensor also exhibited high selectivity for the determination of Pb(II) in the presence of other interfering metal ion. At the same time, the stability of the imprinted layer made the sensor applicable to the detection environment with a pH of 6.4–8.0. Moreover, the sensor was successfully applied to the detection of Pb(II) in mantis shrimp.

Based on dual-template molecular imprinting polymerization technology, a fluorescent molecularly imprinted polymer doped with CdSe/ZnS quantum dots was developed to construct a “Turn-on” fluorescence sensor for the rapid, sensitive, and specific detection of two biogenic amines. The biogenic amines bind to the quantum dots, which eliminates surface defects and enhances the fluorescence emission intensity of the quantum dots. By optimizing both the polymerization and detection processes, the results demonstrate that the sensor can detect biogenic amines within the range of 0.01–10 mmol/L, with a low detection limit of 14.57 μmol/L and a detection time of only ten minutes. Moreover, the sensor is cost-effective and does not require specialized instrument operation, offering a practical approach for the rapid detection of biogenic amines in complex food matrices. This study advances the development of simultaneous recognition and rapid detection technologies for multiple target molecules.

In this study, a dispersive solid-phase extraction (DSPE) pretreatment procedure using zeolite H-Beta as a sorbent was exploited for the determination of eight neonicotinoids in bottled water and honey products based on ultra-high-performance liquid chromatography–tandem mass spectrometry analysis. The zeolite H-Beta was demonstrated to be a suitable sorbent for neonicotinoid insecticides, even after 10 recycles of reuse. The method performance was evaluated by the linearity (R² ≥ 0.998), recovery (71–108%), precision (0.1–7.8%), limit of detection (0.05–0.1 ng/mL) and limit of quantification (0.1–0.2 ng/mL), which suggested excellent stability and high sensitivity with the use of the DSPE procedure. The method was further successfully applied in the test of neonicotinoid insecticides in 34 samples. Zeolite H-Beta shows promise as an efficient and practical material for monitoring neonicotinoid insecticides in bottled water and multiplex honey matrices.