This research presents a thorough analysis of the nutrients and anti-nutrients contained in different products from Algerian carob tree, Ceratonia siliqua L. (pod, pulp, seeds and leaves). Other parameters such as moisture, ash content and calorific value were also determined. The main purpose was to characterize these products derived from carob tree and analyze their possible valorization in several fields such as chemical, energy or medical industries. The results show that these products contain low amount of crude lipids (2.4–4.5%), moderate amounts of protein (4–7.4%) and high levels of total sugar (up to 66.6%). The compositional analysis reveals high nutritional values but, at the same time, it is also worth underscoring their high content in anti-nutrients: (i) trypsin inhibitor (6.4–7.3 mg·g⁻¹); and (ii) phytic acid (0.6–0.94 mg·g⁻¹). This work quantifies these two secondary metabolites in carob tree products for the first time. Finally, based on the results obtained, a process scheme is proposed for the complete use of carob tree products, including the use of anti-nutrients (trypsin inhibitors and phytic acid) for bio-medical applications since many research studies support that these compounds have great potential in this field.

Microbial fuel cells (MFCs) are an environmentally friendly technology that can recover electricity directly from several wastes at ambient temperatures. This work explores the use of mineral oil refinery wastewater as feedstock in single-chamber air-cathode MFC devices. A polymer inclusion membrane based on the ionic liquid methyltrioctylammonium chloride, [MTOA⁺][Cl⁻], at a concentration of 70% w/w, was used as separator, showing a good efficiency in power production and chemical oxygen demand (COD) removal. The power and the chemical oxygen demand removal reached values of 45 mW/m³ and over 80%, respectively. The evolution of other parameters of the wastewater including nitrites, phosphates and sulphates were also studied. Kjeldahl nitrogen and sulphates were significantly reduced during MFC operation. The results show that mineral oil refinery wastewater can be used as feedstock in air breathing cathode-microbial fuel cells based on polymer ionic liquid inclusion membranes. This configuration could represent a good alternative for wastewater depuration while producing energy during the process.