In petroleum-contaminated aquifers, iron (III) serves as an electron acceptor, enabling microbial degradation of organic matter. While previous studies have focused on iron reduction and organic matter degradation under laboratory conditions, research on iron-associated microorganisms in petroleum-contaminated aquifers is limited. To explore the diversity and distribution of such microorganisms in natural settings, this study used metagenomic analysis of an iron-rich, petroleum-contaminated aquifer. Sixteen groundwater samples from both pollution source and background areas were collected for species annotation and functional gene identification. Results show more than 7000 species were identified as iron-reducing microorganisms (IRMs), including several previously well-characterized iron-reducing species (e.g., Geobacter luticola and Geobacter sulfurreducens). However, the majority of IRMs were not found in existing iron-reducing microbial databases. Some of them, such as Sulfurospirillum sp. and Extensimonas perlucida, could be taxonomically classified at the species level, while most were only annotated as unclassified bacteria. In the contamination source zone, these microorganisms proliferated extensively, which led to hydrocarbon degradation predominantly driven by iron reduction in the aquifer. This study enhances our understanding of hydrocarbon-degrading microorganisms and supports the management of petroleum-contaminated sites.

An increasing number of studies have demonstrated that natural source zone depletion (NSZD) in the vadose zone accounts for the majority (90%~99%) of the natural attenuation of light non-aqueous phase liquid (LNAPL). Until now, 0.05 to 12 kg/a.m² NSZD rates at tens of petroleum LNAPL source zones have been determined in the middle or late evolution stage of LNAPL release, in which limited volatile organic compounds (VOCs) and methane (CH₄) were detected. NSZD rates are normally estimated by the gradient method, yet the associated functional microbial activity remains poorly investigated. Herein, the NSZD at an LNAPL-releasing site was studied using both soil gas gradient methods quantifying the O₂, CO₂, CH₄, and VOCs concentrations and molecular biology methods quantifying the abundance of the pmoA and mcrA genes. The results showed that the methanogenesis rates were around 4 to 40 kg/a.m². The values were greater than the rates calculated by the sum of CH₄ escaping (0.3~1.2 kg/a.m²) and O₂ consuming (3~13 kg/a.m²) or CO₂ generating rates (2~4 kg/a.m²), suggesting that the generated CH₄ was oxidized but not thoroughly to CO₂. The functional gene quantification also supported the indication of this process. Therefore, the NSZD rates at the site roughly equaled the methanogenesis rates (4~40 kg/a.m²), which were greater than most of the previously studied sites with a 90th percentile value of 4 kg/a.m². The study extended the current knowledge of the NSZD and has significant implications for LNAPL remediation management.

The frequent occurrence of urban flood disasters is a major and persistent problem threatening the safety of cities in China and elsewhere in the world. As this issue is so pervasive, exploring new methods for more effective risk prevention and urban flood disaster control is now being prioritized. Taking the case of the city of Zhengzhou as an example, this paper proposes using geological, hydrogeological, ecological, and environmental conditions together with appropriate engineering designs to address the problem of urban flooding. The strategy includes integrating urban sponge–hydrogeological conditions, ecological engineering, and the construction of deep underground water storage facilities. Field investigations, data collection and analysis, in situ observations, testing, and laboratory experiments, are analyzed to explain the formation mechanism and means to mitigate flood disasters in Zhengzhou. Our results suggest that the appropriate use of geological, ecological, and hydrogeological aspects, combined with effective engineering practices, can significantly improve the city’s flood control capacity. These measures can solve the problem of the “once-in-a-millennium” occurrence of torrential rain disasters such as the “720” torrential rainstorm that has affected the city of Zhengzhou.

Introduction: Groundwater is considered the best candidate for drinking water supply in the karst area. The groundwater water resources, however, are vulnerable to pathogenic microorganism contamination because of the typically thin soil layers overlying aquifers and the high permeability of the aquifer host rock, resulting in short residence times and low natural attenuation capacities. Until now, little attention has been paid to the critical environmental factors affecting the pathogenic microorganism contamination in soil-groundwater systems in the karst area.Methods: In the study, orthogonality column experiments with controlling ambient temperatures, pH values of inlet water, and soil porosities were carried out to investigate the transport and lifespan of pathogenic microorganisms in the leachate of agricultural soils in the karst area of Yunnan province, China. The pathogenic indicators, i.e., total bacteria count (TBC) and total coliforms count (TCC), and hydrochemical parameters, i.e., pH and permanganate index (COD_Mn) in the leaching water, were systematically monitored.Results and Discussion: The results showed that bacteria including coliforms can survive for prolonged periods of time in karst soils. The soils overlying the karst rocks were unable to impede the bacteria from seeping into the groundwater. The soils, in turn, likely served as both reservoirs and incubators for pathogenic bacteria. The ambient temperature was the most predominant influential factor affecting both TBC and TCC. The bacteria concentrations were proportional to the temperature in the leachate. Therefore, more attention should be paid to temperature variations in protecting the water supply, particularly in the high-temperature period, such as during the summer months.

The Xiaojiang watershed in Luxi, Yunnan, is a typical rocky desertification area, in which karst groundwater pollution is severe and water resources are scarce. This article takes the watershed as an example and investigates the response mechanisms of surface karst spring water quality to agricultural pollution in rocky desertification areas. Specifically, the study was conducted as follows: (I) A total of 108 water samples from 54 sources were collected during the dry and wet seasons for analysis. (i) Principal component and correlation analyses identified the main pollution indicators in the soil surface karst zone of the area, including total bacterial count, total coliforms, COD, pH, and redox potential. (ii) It was also discovered that surface soil, impacted by agricultural activities, directly contributes to groundwater pollution in the soil surface karst zone. (II) Local soil was used to prepare soil columns under various conditions for simulation. The findings indicate: (i) Temperature significantly affects the surface karst springs, with higher temperatures leading to more pronounced water quality responses, increased enrichment of pathogen-microbiota indicators, and degraded water quality. (ii) Soil porosity substantially influences the water quality of surface karst springs. Increased porosity results in looser soil, more oxidizing conditions in the storage matrix, reduced pathogen-microbiota development, and consequently, less water pollution. This study offers theoretical and technical references for evaluating, monitoring, and issuing early warnings for pathogenic bacteria-microbiota pollution in groundwater in rocky desertification areas.

Based on the viscoelastic artificial boundary theory and the equivalent seismic load input method, this study develops a three-dimensional time-domain input method for obliquely incident SV waves; the validation of this input method was verified, and seismic response analysis was conducted on a biased rock tunnel. The results indicate that the structural seismic response under oblique wave incidence differs significantly from that under vertical incidence. With an increase in the incidence angle, the tunnel’s stress, acceleration, and damage zones all tend to concentrate toward the left arch foot and waist. At different times during the earthquake, stresses and plastic zones develop at the tunnel shoulder, along with the obliquely incident seismic wave propagation, and the stresses and plastic zones gradually concentrate in the direction facing the waves, causing damage at the tunnel foot near the earthquake source. Therefore, in tunnel structures located near the epicenter of an earthquake, the damage evolution at the foot of the tunnel facing the seismic waves should garner more attention.