Objectives: Vanadium compounds have various pharmacologic effects and all available evidence reveals that the effects of vanadium compounds depend on many factors, mainly on the type of cells and dose. The proapoptotic or antiapoptotic effect of vanadium compounds depends strongly on the cell type. Materials and Methods: In this study, the effects of vanadium pentoxide (V2O5) were investigated using several tumor cell lines: a colorectal cancer cell line (Colo-205), a human breast adenocarcinoma cell line (MCF-7), and a normal human fibroblast cell line. Five different concentrations of V2O, between 25-200 mu M were applied on the cells and xCELLigence real-time cell analysis was conducted to evaluate the impedance alterations. This study is the first to show V2O5's effects on Colo-205 and MCF-7 and human fibroblast cell lines in a real-time manner. Results: In the Colo-205 cell line, cell index (CI) alterations decreased slightly at 25 mu M and 50 mu M, and increased at 100 mu M, 150 mu M and 200 mu M concentrations. In the MCF-7 cell line, CI alterations increased at all concentrations compared with the untreated control. However, in the healthy fibroblast cell line, the CI alterations decreased at all concentrations compared with the untreated control, which limits the use of V2O5 for its cytotoxic effect in vivo. Conclusion: The combination of conventional anticancer drugs can be used to increase the effectiveness and reduce the adverse effects of these drugs considering stages of cancer and cancer type. Our results suggest that V2O5 has disparate effects on several cancer cells at different concentrations.

Lung adenocarcinoma is one of the most commonly occurring cancer types, leading to cancer-related deaths worldwide. α-Chaconine and α-solanine are the glycoalkaloids found in Potato (Solanum tuberosum L.). In this study, we aim to investigate continuously monitoring the cytotoxicity of API-1, α-chaconine, α-solanine on human lung carcinoma (A549) and human bronchial epithelial (Beas-2b) cell lines. To investigate the cytotoxic effects of these molecules real time cell analyzer xCELLigence system was used. A549 (12.500 cells/well) and Beas-2b (10.000 cells/well) cells were seeded in E-plate then approximately 24 h post-seeding when the cells were in the log growth phase, the cells were treated with these molecules. Cell viability was observed during 48 h after treatment and IC50 values have been calculated. According to results, for A549 cells IC50α-solanine were 12.3 μM and 11.79 μM, and for Beas-2b cells IC50α-solanine were 13.6 μM and 13.3 μM at 24th and 48th h respectively. α-Solanine and API-1 (25 μM) have cytotoxic effects both cell lines at all concentrations. α-Chaconine has no cytotoxic and anti-proliferative effect. These molecules and data provide new information for anticancer studies. In further studies, we are planning to improve our research with identifying the action mechanisms of these compounds on A549 and Beas-2b.