This study is to demonstrate the performance of a new technology for complete nitrogen removal from soybean wastewater with high nitrogen concentrations. Nitrification-denitrification via nitrite was performed in three kinds of operating condition, i.e. nitrification-denitrification via nitrite under traditional SBR process, nitrification-denitrification via nitrite by alternating oxic/anoxic under fixed-time control and nitrification-denitrification via nitrite by alternating oxic/anoxic under on-line fuzzy control.The best one is nitrification-denitrification via nitrite by alternating oxic/anoxic under on-line fuzzy control. It not only improves both nitrification and denitrification rates and decreases total reaction time, but also saves the amount of added carbon source and alkalinity. In addition, reaction time can be shortened and operation cost can be saved by using real-time fuzzy control aeration and mixing time. So this method enhances the efficiency and the stability of nitrogen removal, and reduces operating costs and construction investment in the process of wastewater treatment.