Modern broiler chickens have ongoing bone health problems. Phosphorus (P) plays an important role in bone development and increased understanding of P metabolism should improve the skeletal health of broilers. Enterococcus faecium has been widely used as a probiotic in broiler production and is shown to improve skeletal health of rats, but its effect on the bones of broilers remains unclear. This study investigated the effect of E. faecium on P absorption and utilization in broilers and the associated changes in the gut microbiota using 16S rDNA sequencing. Dietary supplementation with E. faecium improved P absorption through upregulation of the expression of intestinal NaP-IIb mRNA and increased the concentration of serum alkaline phosphatase. These actions increased P retention and bone mineralization in E. faecium-treated broilers. The positive effects of E. faecium on P metabolism were associated with changes in the populations of the intestinal microbiota. There was increased relative abundance of the following genera, Alistipes, Eubacterium, Rikenella and Ruminococcaceae and a decrease in the relative abundance of Faecalibacterium and Escherichia-Shigella. Dietary supplementation with E. faecium changed gut microbiota populations of broilers, increased the relative abundance of SCFA (short-chain fatty acid)-producing bacteria, improved intestinal P absorption and bone forming metabolic activities, and decreased P excretion. E. faecium facilitates increased utilisation of P in broilers.

Background: Immunological stress decreases feed intake, suppresses growth and induces economic losses. However, the underlying molecular mechanism remains unclear. Label-free liquid chromatography and mass spectrometry (LC-MS) proteomics techniques were employed to investigate effects of immune stress on the hepatic proteome changes of Arbor Acres broilers (Gallus Gallus domesticus) challenged with Escherichia coli lipopolysaccharide (LPS). Results: Proteomic analysis indicated that 111 proteins were differentially expressed in the liver of broiler chickens from the immune stress group. Of these, 28 proteins were down-regulated, and 83 proteins were up-regulated in the immune stress group. Enrichment analysis showed that immune stress upregulated the expression of hepatic proteins involved in defense function, amino acid catabolism, ion transport, wound healing, and hormone secretion. Furthermore, immune stress increased valine, leucine and isoleucine degradation pathways. Conclusion: The data suggests that growth depression of broiler chickens induced by immune stress is triggered by hepatic proteome alterations, and provides a new insight into the mechanism by which immune challenge impairs poultry production.

Large amounts of spent mushroom substrate (SMS) are produced globally, but their utilization efficiency is low, which leads to negative environmental impacts, such as water, soil, and air pollution. SMS contains nutrients, such as cell proteins, with a potential application in animal feed. However, the lignocellulose in SMS restricts animal digestion and absorption, thus hindering its application in animal nutrition. We investigated the potential of cellulase, xylanase, β-galactosidase, and a variety of microorganisms to optimize the conditions for reducing sugars’ (RS) production and the degradation rate of neutral detergent fibers. The results showed that the optimum proportion of multiple enzymes for glucose production of up to 210.89 mg/g were 10% cellulase, 10% xylanase, and 2% β -galactosidase, at 50 °C and 60% moisture for a 20 h hydrolysis duration. To enhance the optimal enzymolysis combination, co-fermentation experiments with multiple microorganisms and enzymes showed that inoculation with 10% Bacillus subtilis, 2% Pediococcus acidilactici, and 2% Saccharomyces cerevisiae, in combination with 10% cellulase, 10% xylanase, 2% β-galactosidase, and 1% urea, at 36.8°C and 59% moisture for 70 h hydrolysis, could lead to a 23.69% degradation rate of the neutral detergent fiber. This process significantly increased the degradation rate of the neutral detergent fiber and the nutrient content of Pleurotus eryngii compared to the initial fermentation conditions. Overall, our study generated optimal co-fermentation conditions for bacteria and enzymes and provides a practical reference for biological feed synthesis using P. eryngii spent mushroom substrate.

The aryl hydrocarbon receptor (AhR) is a transcription factor that regulates the immune system through complicated transcriptional programs. Genistein, an AhR ligand, exhibits anti-inflammatory properties. However, its role in modulating immune responses via the AhR signaling pathway remains unclear. In this study, 360 male Arbor Acre broilers (1-day-old) were fed a basal diet supplemented with 40 or 80 mg/kg genistein and infected with or without Clostridium perfringens (Cp). Our results demonstrated that genistein ameliorated Cp-induced intestinal damage, as reflected by the reduced intestinal lesion scores and improved intestinal morphology and feed-to-gain ratio. Moreover, genistein increased intestinal sIgA, TGF-β, and IL-10, along with elevated serum IgG, IgA, and lysozyme levels. Genistein improved intestinal AhR and cytochrome P450 family 1 subfamily A member 1 (CYP1A1) protein levels and AhR⁺ cell numbers in Cp-challenged broilers. The increased number of AhR⁺CD163⁺ cells in the jejunum suggested a potential association between genistein-induced AhR activation and anti-inflammatory effects mediated through M2 macrophage polarization. In IL-4-treated RAW264.7 cells, genistein increased the levels of AhR, CYP1A1, CD163, and arginase (Arg)-1 proteins, as well as IL-10 mRNA levels. This increase was attenuated by the AhR antagonist CH223191. In summary, genistein activated the AhR signaling pathway in M2 macrophages, which enhanced the secretion of anti-inflammatory cytokines and attenuated intestinal damage in Cp-infected broilers Cp.