Objectives: To slow down the chemical fixation of phosphate fertilizer, reduce the risk of active phosphorus leaching, stimulate the inherent phosphorus resource activity of soil, and improve phosphorus supply capacity. Methods: This study utilized a combination of field experiments and indoor chemical analysis. Six types of exogenous organic matter (fulvic acid, biochar, compound microbial fertilizer, high-energy microbial inoculum, pig manure-vermicompost, cow manure-vermicompost) were added based on conventional fertilization. The experiment was conducted under the wheat-maize rotation system in the Huang-Huai-Hai region. Results: Compared with control (CK) without exogenous organic matter (EOM), all the other treatments with EOM had an enhancing effect on the available phosphorus of the cultivated soil. During the maize harvest, the combined application of biochar, pig manure-vermicompost and cow manure-vermicompost treatment significantly increased the content of available phosphorus in 0–20 cm soil by 45.87–56.59% compared with CK. The combined application of fulvic acid, biochar, pig manure-vermicompost and cow manure-vermicompost treatment significantly increased the content of Ca₂-P in 0–20 cm soil by 34.04–65.14%. The content of Ca₁₀-P in each treatment with EOM exhibited a lower level compared to CK. EOM could slow down the fixation of phosphorus to some degree. Correlation analysis revealed significant associations between Ca₂-P, Ca₈-P, Al-P, Fe-P, neutral phosphatase activity, acid phosphatase activity, and the available phosphorus content in the soil. The combined application of fulvic acid, biochar, and cow manure-vermicompost could enhance the activity of neutral and acid phosphatase in topsoil to a certain extent, thereby facilitating the conversion of phosphorus into highly available Ca₂-P. EOM could enhance the soil phosphorus availability and decelerate the conversion of soil phosphorus into O-P and Ca₁₀-P forms with low availability. Among all treatments, biochar exhibited the most pronounced efficiency in mitigating phosphorus leaching downward. Conclusions: All the EOMs had the potential to enhance the conversion of phosphorus into soluble phosphorus (Ca₂-P), thereby mitigating the chemical fixation of soil phosphorus and ameliorating non-point source pollution caused by phosphorus. EOM enhanced the activity of neutral and acid phosphatase, which was beneficial to the conversion of organic phosphorus to inorganic phosphorus and increasing the content of available phosphorus. All EOMs had good effects on the retention of soil effective phosphorus, among which biochar had the best effect on retaining effective phosphorus in the tillage layer and blocking phosphorus leaching downward.

To achieve a scientific and objective evaluation of soil acidification, secondary salinization, and nutrient imbalance in old protected vegetable fields (OPVs) with over 30 years of cultivation history, a soil surface breeding vigorous moss was investigated. Here, quantitative laboratory analysis and mathematical statistics were employed to explore the spatial distribution of soil salinity and nutrients, as well as their relationships. The results revealed that OPVs exhibited slightly acidified values. The measured anions and cations in the soil salt composition constituted approximately 77% of the total ions. Among which, Ca²⁺ was the dominant cation, while SO₄²⁻ and NO₃⁻ were predominant anions. The total water-soluble salt (TDS) content of the surface soil reached 4.52 g kg⁻¹, exceeding the Chinese Saline Soils standard (1.0 g kg⁻¹) by 350%. In the OPVs, nitrate nitrogen was significantly higher than ammonium nitrogen, and available phosphorus and available potassium were generally abundant. Despite exhibited various soil health concerns, a field visit survey presented consistently high and stable yields in OPVs. We hypothesize that this seemingly contradictory finding may be attributable to several factors, including the abundance of divalent cations (Ca²⁺ and Mg²⁺), the soil fertility and water retention capacity of unsaturated salt-based suitable soil, as well as good soil aggregate structure. These factors had the potential to reduce the stresses on the soil. This study provided a foundational understanding of the nutrient and salinity status of soils in OPVs, offering valuable data and theoretical groundwork for future research endeavors.

Plants commonly encounter spatially heterogeneous distributions of nitrogen (N) forms and iron (Fe) in soils, but how N form modifies plant responses to heterogeneous Fe availability remains poorly understood. Using a split-root system, we examined the phenotypic responses of Arabidopsis thaliana and wheat to contrasting Fe availability combined with ammonium (NH₄⁺) or nitrate (NO₃⁻) supply. Plants were exposed to high (100 μM) or low (5 μM) Fe in combination with NH₄⁺ or NO₃⁻, either under homogeneous or spatially heterogeneous conditions. Within each species, NH₄⁺ and NO₃⁻ treatments were supplied at equivalent total N concentrations. In both species, high Fe combined with sole NH₄⁺ supply resulted in pronounced reductions in shoot and root growth, whereas spatial presence of NH₄⁺ and NO₃⁻ between root compartments was associated with improved growth. Under high-Fe conditions, sole NH₄⁺ supply to both root compartments (HAHA) resulted in pronounced reductions in shoot and root growth, whereas supplying NH₄⁺ and NO₃⁻ to separate root compartments (HAHN) was associated with improved growth. Under these conditions, shoot fresh weight in HAHN was 178% higher than that in HAHA in Arabidopsis and 231% higher in wheat. In wheat, root fresh weight under HAHN was 471% higher than under HAHA. Wheat exhibited stronger spatial differentiation of root growth between contrasting nutrient compartments, whereas Arabidopsis showed greater variation in whole-plant growth in response to N form. Chlorophyll accumulation also showed species- and Fe-dependent responses to N form. Overall, these results indicate that N form modifies plant phenotypic responses to heterogeneous Fe–N supply and that wheat exhibits greater root plasticity under spatially heterogeneous Fe–N conditions. The roles of plant Fe and N status, rhizosphere pH, and long-distance signaling in these responses remain to be determined.