We investigated the mineral concentrations of 61 honey samples from eight Romanian regions, employing advanced techniques to assess 30 chemical elements. Potassium emerged as the dominant element, showcasing significant variations across geographical locations. Essential minerals like calcium, magnesium, sodium, and manganese maintained consistent levels, while zinc, copper, and chromium were present in smaller proportions. Critically, lead and cadmium levels exceeded established safety limits in some samples, suggesting potential environmental contamination. Additionally, elevated levels of lithium, strontium, nickel, and aluminum were detected, hinting at possible atmospheric pollution. These findings highlight the importance of regional analysis, as mineral content varied significantly between locations. Furthermore, correlation analysis revealed interdependencies among elements, suggesting shared environmental influences. Advanced statistical techniques like hierarchical clustering and principal component analysis effectively captured the impact of geographical origin on honey composition. These insights contribute valuable information for future efforts in honey quality control, traceability systems, and regulatory measures. By providing valuable insights into environmental influences on honey composition, this study informs future research endeavors and paves the way for the development of robust regulatory measures to ensure honey safety for consumers.

Evaluating cultivar susceptibility to biotic stressors in apple orchards is essential for selecting genotypes adapted to local conditions and for designing effective plant protection strategies. This study conducted a comparative assessment of five apple cultivars (‘Florina’, ‘Jonathan’, ‘Golden Delicious’, ‘Pinova’, and ‘Idared’) in response to major fungal diseases (Venturia inaequalis, Podosphaera leucotricha, and Monilinia spp.) and insect pests (Eriosoma lanigerum, Quadraspidiotus perniciosus, Anthonomus pomorum, Aphis spp., and Cydia pomonella). The cultivars were monitored over a five-year period in six orchards located in Central Transylvania, Romania. Significant differences in phytosanitary behavior were recorded among cultivars and locations. ‘Florina’ consistently showed the highest tolerance to pathogens and pests across all sites and years, while ‘Jonathan’ and ‘Golden Delicious’ proved highly susceptible, particularly to apple scab, powdery mildew, aphids, and codling moth. Pest incidence was strongly influenced by temperature, while disease occurrence was more closely linked to precipitation patterns. Heritability analysis indicated that genetic factors played a substantial role in shaping cultivar responses to most biotic stressors. The integrated approach to cultivar–location–pathogen and pest interactions offers practical insights for optimizing orchard protection strategies under variable ecological conditions.

The gut microbiome is central to immune, metabolic, and gastrointestinal health across species. Dysbiosis disrupts microbial communities and is linked to inflammatory bowel disease, celiac disease, and other immune-mediated gastrointestinal disorders. This review addresses the central question of how diet- and environment-driven gut dysbiosis in farm animals and fish is transmitted through the food chain to influence human gastrointestinal health within a One Health framework. This review synthesizes recent evidence within the One Health framework, focusing on how diet- and environment-induced dysbiosis in farm animals and fish can influence human gastrointestinal health via the food chain. We highlight mechanisms of immune modulation, alterations in food products, and the risks of pathogen transmission and antimicrobial resistance. An important limitation of the current body of evidence is the lack of studies that comprehensively trace the proposed axis from animal gut dysbiosis to human health outcomes. Emerging interventions, including precision nutrition, probiotics, and microbiota-targeted therapies, show potential for restoring microbial balance, though translating these findings into clinical practice remains challenging. By integrating human, veterinary, and environmental perspectives, this work proposes a novel cross-species microbiome–diet–immune framework to guide future research and interventions, advancing One Health strategies for disease prevention, antimicrobial resistance mitigation, and sustainable gastrointestinal health.

Pear fruit quality is a key determinant of consumer preference, yet it remains insufficiently characterized in many newly developed cultivars. This study aimed to evaluate 25 pear genotypes (Pyrus communis L.), internationally renowned cultivars and new cultivars developed through Romanian breeding programs, with distinct ripening periods, using an integrative approach based on morphological, biochemical, and sensory traits. Standardized methods were applied to assess attributes including fruit size, firmness, soluble solids, organic acid composition, skin color, and hedonic sensory responses for taste, aroma, texture, and visual appeal. Results revealed significant variability across ripening groups, with several cultivars, such as ‘Paradox’, ‘Pandora’, ‘Isadora’, and ‘Daciana’, displaying favorable combinations of appearance, internal quality, and consumer-rated acceptability. ‘Paradox’ and ‘Pandora’ achieved the highest sensory scores, comparable to or surpassing those of commercial standards like ‘Packham’s Triumph’. Multivariate analyses confirmed trait correlations and distinct phenotypic profiles among cultivars. These findings underscore the utility of multidimensional quality assessment for selecting cultivars suited to modern market demands, both for fresh consumption and processing. Moreover, several genotypes demonstrated potential as parental sources in pear breeding programs targeting improved organoleptic and physicochemical traits. The study suggests that a differentiated sensory scoring approach, rather than a uniform 1–9 hedonic scale, may more effectively identify high-quality pear cultivars for breeding programs.