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Black locust (Robinia pseudoacacia L.) was among the first North-American tree species imported in Europe. In Romania, black locust has established itself as a forest tree appreciated for multiple uses. The objective of the hereby study was to identify a quality planting material at black locust using seeds from trees with superior traits from five stands geographically close, located in North-western of Romania. An empirical selection was done, thus trees with the most favourable traits were selected as plus trees. Among the averages of the main traits (tree height, diameter at breast height, basal area, self pruning trunk length, crown diameter) of the plus trees from the five stands, there were registered significant differences, and two stands stood out with a high biomass growth. Even if the stands had different ages (between 20-35 year), the age did not influence significantly the growth traits of the trees. The seeds of the plus trees (open-pollinated) from all the stands had large size (mean seed weight of 0.057 g/seed). The seedling emergence rate was high, especially in the solarium condition (between 52.7-73.7% compared with 33.0-41.3% in the field). Coefficient of genetic correlation and heritability calculated for the seedlings belongings to half-sib families highlighted that black locust breeding can be extremely effective by a proper selection.
Andrea M. Roman; Irina M. Morar; Alina M. Truța; Cătălina Dan; Adriana F. Sestraș; Liviu Holonec; Florin Ioras; Radu E. Sestras. Trees, seeds and seedlings analyses in the process of obtaining a quality planting material for black locust (Robinia pseudoacacia L.). Notulae Scientia Biologicae 2020, 12, 940 -958.
AMA StyleAndrea M. Roman, Irina M. Morar, Alina M. Truța, Cătălina Dan, Adriana F. Sestraș, Liviu Holonec, Florin Ioras, Radu E. Sestras. Trees, seeds and seedlings analyses in the process of obtaining a quality planting material for black locust (Robinia pseudoacacia L.). Notulae Scientia Biologicae. 2020; 12 (4):940-958.
Chicago/Turabian StyleAndrea M. Roman; Irina M. Morar; Alina M. Truța; Cătălina Dan; Adriana F. Sestraș; Liviu Holonec; Florin Ioras; Radu E. Sestras. 2020. "Trees, seeds and seedlings analyses in the process of obtaining a quality planting material for black locust (Robinia pseudoacacia L.)." Notulae Scientia Biologicae 12, no. 4: 940-958.
Assessment of the diversity of reproductive traits and genetic variation is of great relevance to the conservation of genetic resources and management of silver fir (Abies alba) populations. We have evaluated reproductive characteristics associated with female cones and seed morphology, as well as seed germination after subjecting seeds to five storage methods, in nine Romanian populations of A. alba. The genetic diversity of the populations was assessed with 12 polymorphic simple sequence repeat (SSR) markers. We detected significant differences between populations for all reproductive traits and considerable differences in seed germination and storage methods; seed storage in wet sand was the method resulting in the highest germination in all populations. Genomic SSRs (gSSRs) were more informative on average than expressed sequence tag SSRs (EST-SSRs) in the populations studied. The nine populations were genetically diverse, with an average number of alleles (N) per SSR locus between 3.50 and 4.83. The observed heterozygosity (Ho) in the nine populations was always lower than the expected heterozygosity (He), which resulted in values of the inbreeding coefficient (Fis) between 0.261 and 0.709. Genetic distances between populations ranged between 0.077 and 0.410. The cluster analysis based on genetic distances did not group accessions according to their geographical proximity, and despite a positive trend, the correlation between geographic and genetic distances was non-significant. The results of an analysis of molecular variance (AMOVA) revealed that only 9.1% of the total molecular variance is attributable to differences between populations. This low degree of genetic differentiation between populations is confirmed by the intermingling of individuals of different populations in a principal coordinate analysis (PCoA). We found evidence of a positive relationship between He and germination, as well as a negative one between Fis and germination, suggesting that populations with low diversity and high consanguinity may have a reduced fitness and long-term viability. The results are relevant for the conservation and management of local genetic resources and populations, as well as for reforestation programmes of silver fir.
Irina Maria Todea Morar; Stephanie Rensen; Santiago Vilanova; Monica Boscaiu; Liviu Holonec; Adriana F. Sestras; Oscar Vicente; Jaime Prohens; Radu E. Sestras; Mariola Plazas. Genetic Relationships and Reproductive Traits of Romanian Populations of Silver Fir (Abies alba): Implications for the Sustainable Management of Local Populations. Sustainability 2020, 12, 4199 .
AMA StyleIrina Maria Todea Morar, Stephanie Rensen, Santiago Vilanova, Monica Boscaiu, Liviu Holonec, Adriana F. Sestras, Oscar Vicente, Jaime Prohens, Radu E. Sestras, Mariola Plazas. Genetic Relationships and Reproductive Traits of Romanian Populations of Silver Fir (Abies alba): Implications for the Sustainable Management of Local Populations. Sustainability. 2020; 12 (10):4199.
Chicago/Turabian StyleIrina Maria Todea Morar; Stephanie Rensen; Santiago Vilanova; Monica Boscaiu; Liviu Holonec; Adriana F. Sestras; Oscar Vicente; Jaime Prohens; Radu E. Sestras; Mariola Plazas. 2020. "Genetic Relationships and Reproductive Traits of Romanian Populations of Silver Fir (Abies alba): Implications for the Sustainable Management of Local Populations." Sustainability 12, no. 10: 4199.
The Transylvanian region of Romania is a place of rich history since ancient times, where the original natural environment around architectural heritage sites or buildings has not been severely altered by urban development. Unfortunately, many such places are left by the authorities to degrade or totally collapse for lack of funds, vision or initiatives. The current paper addresses the potential of Unmanned Aerial Vehicles (UAVs) in the assessment of a viable and feasible prospect of restoration on a 19th century mansion that belonged to a nobiliary family. UAV use is rising in many industries and has become very popular in the last decade, but for survey engineering and related domains they represent a quantum leap in technology. Integrating UAV-acquired data and structure from motion software, has enabled modern techniques to obtain useful metrics from the field, accurate photorealistic 3D models for visual inspection, structural damage analyses, architectural rehabilitation-restoration, conservation and spatial analysis of the surrounding area. In this work a socio-cultural planning and design process is explored and presented to improve the local community and inclusion in a tourist circuit based on the regional potential, as well as an evaluation of accessibility derived from a vector-raster database that highlights the central position of the cultural heritage in regards to the axis of circulation between the important metropolitan areas and the local tourist attractions. This established workflow of modern topographic and construction measurements is fully integrable into the architectural process, building information modelling, heritage conservation and reconstruction.
Paul Sestras; Sanda Roșca; Ștefan Bilașco; Sanda Naș; Stefan M. Buru; Leontina Kovacs; Velibor Spalević; Adriana F. Sestras. Feasibility Assessments Using Unmanned Aerial Vehicle Technology in Heritage Buildings: Rehabilitation-Restoration, Spatial Analysis and Tourism Potential Analysis. Sensors 2020, 20, 2054 .
AMA StylePaul Sestras, Sanda Roșca, Ștefan Bilașco, Sanda Naș, Stefan M. Buru, Leontina Kovacs, Velibor Spalević, Adriana F. Sestras. Feasibility Assessments Using Unmanned Aerial Vehicle Technology in Heritage Buildings: Rehabilitation-Restoration, Spatial Analysis and Tourism Potential Analysis. Sensors. 2020; 20 (7):2054.
Chicago/Turabian StylePaul Sestras; Sanda Roșca; Ștefan Bilașco; Sanda Naș; Stefan M. Buru; Leontina Kovacs; Velibor Spalević; Adriana F. Sestras. 2020. "Feasibility Assessments Using Unmanned Aerial Vehicle Technology in Heritage Buildings: Rehabilitation-Restoration, Spatial Analysis and Tourism Potential Analysis." Sensors 20, no. 7: 2054.
Forest ecosystems are frequently exposed to abiotic stress, which adversely affects their growth, resistance and survival. For silver fir (Abies alba), the physiological and biochemical responses to water and salt stress have not been extensively studied. Responses of one-year-old seedlings to a 30-day water stress (withholding irrigation) or salt stress (100, 200 and 300 mM NaCl) treatments were analysed by determining stress-induced changes in growth parameters and different biochemical markers: accumulation of ions, different osmolytes and malondialdehyde (MDA, an oxidative stress biomarker), in the seedlings, and activation of enzymatic and non-enzymatic antioxidant systems. Both salt and water stress caused growth inhibition. The results obtained indicated that the most relevant responses to drought are based on the accumulation of soluble carbohydrates as osmolytes/osmoprotectants. Responses to high salinity, on the other hand, include the active transport of Na+, Cl− and Ca2+ to the needles, the maintenance of relatively high K+/Na+ ratios and the accumulation of proline and soluble sugars for osmotic balance. Interestingly, relatively high Na+ concentrations were measured in the needles of A. alba seedlings at low external salinity, suggesting that Na+ can contribute to osmotic adjustment as a ‘cheap’ osmoticum, and its accumulation may represent a constitutive mechanism of defence against stress. These responses appear to be efficient enough to avoid the generation of high levels of oxidative stress, in agreement with the small increase in MDA contents and the relatively weak activation of the tested antioxidant systems.
Irina Maria Todea (Morar); Sara González-Orenga; Monica Boscaiu; Mariola Plazas; Adriana F. Sestras; Jaime Prohens; Oscar Vicente; Radu E. Sestras. Responses to Water Deficit and Salt Stress in Silver Fir (Abies alba Mill.) Seedlings. Forests 2020, 11, 395 .
AMA StyleIrina Maria Todea (Morar), Sara González-Orenga, Monica Boscaiu, Mariola Plazas, Adriana F. Sestras, Jaime Prohens, Oscar Vicente, Radu E. Sestras. Responses to Water Deficit and Salt Stress in Silver Fir (Abies alba Mill.) Seedlings. Forests. 2020; 11 (4):395.
Chicago/Turabian StyleIrina Maria Todea (Morar); Sara González-Orenga; Monica Boscaiu; Mariola Plazas; Adriana F. Sestras; Jaime Prohens; Oscar Vicente; Radu E. Sestras. 2020. "Responses to Water Deficit and Salt Stress in Silver Fir (Abies alba Mill.) Seedlings." Forests 11, no. 4: 395.
Drought periods are becoming more frequent and intense, due to the effects of climate change, threatening natural habitats worldwide, including European forests. Forest trees can also be affected by high soil salinity, because of the common practice of de-icing of mountain roads with NaCl in winter. We have evaluated the responses to salt and water stress of silver fir (Abies alba), an important forest species for which very limited information is available. One-year-old fir seedlings, with origin in seven different locations in Romania, were subjected to salt (watering with NaCl solutions of increasing concentrations) and water deficit (complete withholding of irrigation) treatments in the greenhouse. After one month, plant material was harvested and different morphological parameters were determined in the stressed and control plants. Both stress treatments inhibited growth of fir seedlings from all seven provenances, although quantitative differences in the responses to stress were observed between populations. Growth inhibition was established by the relative reduction – as compared to the non-stressed controls - in several parameters, such as stem elongation, root length, number of needles, or fresh weight and water content of roots and needles. Statistical multivariate analysis of the results suggested that seedlings from Valea Morii (population 6) were the most tolerant to both, water deficit and high (300 mM NaCl) salt concentrations. These results support the possibility to screen a large number of individuals from different populations, at the seedling stage, to select Abies alba genotypes with enhanced drought and/or salinity tolerance. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********
Irina M. Todea (Morar); Sara Gonzalez Orenga; Mariola Plazas; Adriana F. Sestras; Jaime Prohens; Oscar Vicente; Radu E. Sestras; Monica Boscaiu. Screening for Salt and Water Stress Tolerance in Fir (Abies alba) Populations. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019, 47, 1063 -1072.
AMA StyleIrina M. Todea (Morar), Sara Gonzalez Orenga, Mariola Plazas, Adriana F. Sestras, Jaime Prohens, Oscar Vicente, Radu E. Sestras, Monica Boscaiu. Screening for Salt and Water Stress Tolerance in Fir (Abies alba) Populations. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2019; 47 (4):1063-1072.
Chicago/Turabian StyleIrina M. Todea (Morar); Sara Gonzalez Orenga; Mariola Plazas; Adriana F. Sestras; Jaime Prohens; Oscar Vicente; Radu E. Sestras; Monica Boscaiu. 2019. "Screening for Salt and Water Stress Tolerance in Fir (Abies alba) Populations." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 47, no. 4: 1063-1072.
European larch (Larix decidua Mill.) has been reported either as more tolerant or as more sensitive to drought than conifers with perennial leaves. Previous studies have revealed that Carpathian populations of European larch display a high genetic variability. A comparative study of the responses of these populations to drought stress at the seedling stage might allow the identification of drought tolerant genotypes and reliable drought stress biomarkers, which could be eventually used for the early detection of drought effects in larch, not only under control greenhouse conditions, but also in their natural stands. Growth responses were analyzed in larch seedlings from six Romanian Carpathian populations, submitted to one month of mild drought stress under controlled conditions. Levels of photosynthetic pigments (chlorophylls a and b, and carotenoids), osmolytes (proline and total soluble sugars), monovalent cations (Na+ and K+), and malondialdehyde (MDA) and non-enzymatic antioxidants (total phenolics and flavonoids) were compared with control treatments and between populations. Growth and the pattern of the biochemical responses were very similar in the six populations. Drought stress lead to stem length decrease in all population, whereas reduction of fresh weight of needles was significant only in one population (BVVC), and reduction of water content of needles in two populations (BVVC and GuHo). The optimal biochemical traits for an early detection of drought symptoms in this species is the increase—in most populations—of total soluble sugars, MDA, and total phenolic compounds, whereas K+ reduction was significant in all populations. Photosynthetic pigments remained unchanged, except for the Anin population where they were reduced under stress. Multivariate principal component and hierarchical clustering analyses confirmed the impact of drought in the growth and physiology of European larch, and revealed that the humidity of the substrate was positively correlated with the growth parameters and the levels of K+ in needles, and negatively correlated with the levels of MDA, total soluble sugars, total phenolic compounds, and flavonoids in needles.
Ioana M. Plesa; Mohamad Al Hassan; Sara González-Orenga; Adriana F. Sestras; Oscar Vicente; Jaime Prohens; Monica Boscaiu; Radu E. Sestras. Responses to Drought in Seedlings of European Larch (Larix decidua Mill.) from Several Carpathian Provenances. Forests 2019, 10, 511 .
AMA StyleIoana M. Plesa, Mohamad Al Hassan, Sara González-Orenga, Adriana F. Sestras, Oscar Vicente, Jaime Prohens, Monica Boscaiu, Radu E. Sestras. Responses to Drought in Seedlings of European Larch (Larix decidua Mill.) from Several Carpathian Provenances. Forests. 2019; 10 (6):511.
Chicago/Turabian StyleIoana M. Plesa; Mohamad Al Hassan; Sara González-Orenga; Adriana F. Sestras; Oscar Vicente; Jaime Prohens; Monica Boscaiu; Radu E. Sestras. 2019. "Responses to Drought in Seedlings of European Larch (Larix decidua Mill.) from Several Carpathian Provenances." Forests 10, no. 6: 511.
Larix decidua, the European larch, does not grow in natural saline areas, but it can be affected by salinity either by the common practice of winter de-icing of mountain roads with NaCl, or when grown as an ornamental tree in urban areas by the use of low quality, salinised irrigation water. In the present study, the responses to salt stress of young seedlings obtained from seeds of seven Carpathian larch populations were analysed. After 30 days of treatments with 150 mM NaCl, growth and biochemical parameters were determinated. Salt stress inhibited growth of all seedlings, as shown by the relative reduction of stem length and fresh weight, and induced significant changes in the needle levels of several biochemical stress markers. Seedlings from all populations showed a marked reduction of photosynthetic pigments contents and an increase of proline and malondialdehyde (MDA) concentrations. Under salt stress, plants accumulated Na+ and Cl- in the needles, whereas K+ was maintained at a steady level. Responses of seedlings from the different populations were similar, with only small quantitative differences that did not allow the identification of more salt tolerant genotypes. However, the study revealed that several of the biochemical markers mentioned above can be suitable for the rapid and non-destructive assessment of the effects of salinity in European larch.
Ioana Plesa; Mohamad AL Hassan; Adriana F. Sestras; Oscar Vicente; Monica Boscaiu; Radu E. Sestras. Biochemical Markers of Salt Stress in European Larch (Larix decidua). Notulae Scientia Biologicae 2018, 10, 430 -438.
AMA StyleIoana Plesa, Mohamad AL Hassan, Adriana F. Sestras, Oscar Vicente, Monica Boscaiu, Radu E. Sestras. Biochemical Markers of Salt Stress in European Larch (Larix decidua). Notulae Scientia Biologicae. 2018; 10 (3):430-438.
Chicago/Turabian StyleIoana Plesa; Mohamad AL Hassan; Adriana F. Sestras; Oscar Vicente; Monica Boscaiu; Radu E. Sestras. 2018. "Biochemical Markers of Salt Stress in European Larch (Larix decidua)." Notulae Scientia Biologicae 10, no. 3: 430-438.
Larix decidua, the European larch, is not normally affected by drought or salinity in its natural habitats, but it may be when grown as an ornamental tree, by the widespread practice of winter de-icing of mountain roads with NaCl, and because of global warming-induced environmental changes. The responses of two-month-old larch seedlings to 30 days water deficit (withholding irrigation) or salt stress (150 mM NaCl) treatments were studied by determining stress-induced changes in several growth parameters and biochemical markers (ion and osmolyte contents, level of oxidative stress, activation of enzymatic and non-enzymatic antioxidant systems). Both treatments caused the inhibition of growth, degradation of photosynthetic pigments, a small increase in malondialdehyde (MDA, an oxidative stress biomarker), and the activation of antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR). In all cases, salinity appeared to have stronger effects on the seedlings than water deficit. The presence of relatively high concentrations of glycine betaine, both in control and stressed plants, may represent a constitutive mechanism of defence against stress in European larch. Additionally, other responses were specific for salt stress and included the activation of K+ transport from roots to shoots and the accumulation of Pro as an osmoprotectant.
Ioana M. Plesa; Sara Gonzalez Orenga; Mohamad Al Hassan; Adriana F. Sestras; Oscar Vicente; Jaime Prohens; Radu E. Sestras; Monica Boscaiu. Effects of Drought and Salinity on European Larch (Larix decidua Mill.) Seedlings. Forests 2018, 9, 320 .
AMA StyleIoana M. Plesa, Sara Gonzalez Orenga, Mohamad Al Hassan, Adriana F. Sestras, Oscar Vicente, Jaime Prohens, Radu E. Sestras, Monica Boscaiu. Effects of Drought and Salinity on European Larch (Larix decidua Mill.) Seedlings. Forests. 2018; 9 (6):320.
Chicago/Turabian StyleIoana M. Plesa; Sara Gonzalez Orenga; Mohamad Al Hassan; Adriana F. Sestras; Oscar Vicente; Jaime Prohens; Radu E. Sestras; Monica Boscaiu. 2018. "Effects of Drought and Salinity on European Larch (Larix decidua Mill.) Seedlings." Forests 9, no. 6: 320.
The influence of rootstock on the growth and fruiting of three sweet cherry cultivars (‘Bigarreau Burlat’, ‘Kordia’, ‘Regina’) was studied under the pedoclimatic conditions of Cluj-Napoca, Romania, in 2014-2016. The trees were grafted on Gisela 5 rootstock, trained as Zahn Spindle and the orchard had a density of 1666 trees/ha. Root were cut twice, at 50 cm distance from the trunk, in an angle of 45° and 30 cm depth, as followed: first time, to the autumn, in fall leaves time, on one side of the row and the second time, in spring, at blooming time, at the other side of the row. The rootstocks influenced height of the trees, the shoot growth, the number of long and fruiting branches, trunk cross sectional area yield and precocity, with differences statistically assured. The longest shoots, in mean values, gave the variant with cultivars grafted on Mahaleb rootstock (111.7 cm). ‘Gisela 5’ rootstock decreases the average length of annual growth (93.3 cm). Trees grafted on Mahaleb formed more long fruiting branches and fewer short fruiting branches than those grafted on ‘Gisela 5’. The biggest average trunks cross sectional area were obtained for the cultivars grafted on Mahaleb (62.1 cm2). Also rootstocks influenced the height of the trees. The cumulative yield was almost double in variants where the trees were grafted on ‘Gisela 5’ (23.2kg/tree), compared to the variants where the trees were grafted on Mahaleb (13.1 kg/tree).
Monica Diana Pal; Ioana Mitre; Adrian C. Asănică; Adriana F. Sestraș; Adrian G. Peticilă; Viorel Mitre. The Influence of Rootstock on the Growth and Fructification of Cherry Cultivars in a High Density Cultivation System. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2017, 45, 451 -457.
AMA StyleMonica Diana Pal, Ioana Mitre, Adrian C. Asănică, Adriana F. Sestraș, Adrian G. Peticilă, Viorel Mitre. The Influence of Rootstock on the Growth and Fructification of Cherry Cultivars in a High Density Cultivation System. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2017; 45 (2):451-457.
Chicago/Turabian StyleMonica Diana Pal; Ioana Mitre; Adrian C. Asănică; Adriana F. Sestraș; Adrian G. Peticilă; Viorel Mitre. 2017. "The Influence of Rootstock on the Growth and Fructification of Cherry Cultivars in a High Density Cultivation System." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 45, no. 2: 451-457.
Norway spruce seedlings apparently showing a relatively higher tolerance to drought can be easily selected using a battery of biomarkers such as water content, chlorophyll, and proline levels in the needles, and could be eventually used as an initial screening method in reforestation programmes. Norway spruce is a native European coniferous species distributed from the Carpathian Mountains and the Alps to northern Scandinavia. In the coming decades, spruce forests will need to cope with increasing climate changes which are already threatening their natural habitats. To identify reliable water stress biomarkers in this species, which may be eventually used to select populations responding better to forecasted drought events, we studied the physiological responses to severe water stress treatments (6-week withholding irrigation in the greenhouse) of 1-year-old spruce seedlings originating from several locations in the Romanian Carpathian Mountains. Variations in the levels of the studied photosynthetic pigments, osmolytes, and non-enzymatic antioxidants were detected across the spruce populations. Several of the parameters determined in seedling needles, such as the decrease in water content (nearly 40% reduction in the most sensitive populations), the degradation of chlorophylls, or a low increase of proline levels (up to sevenfold increment in the most sensitive populations but no change in the most tolerant), could be employed as biomarkers for an early assessment of water stress at this stage. Furthermore, seedlings from two of the populations under study responded better to water stress than the other populations and also seemed to be the least affected by osmotic stress during seed germination. Therefore, the determination of these biochemical markers at early seedling stages could represent a useful tool for the initial screening of populations with relatively high tolerance to drought, warranting further research for their potential use in spruce reforestation programmes.
Sorin T. Schiop; Mohamad Al Hassan; Adriana F. Sestras; Monica Boscaiu; Radu E. Sestras; Oscar Vicente. Biochemical responses to drought, at the seedling stage, of several Romanian Carpathian populations of Norway spruce (Picea abies L. Karst). Trees 2017, 31, 1479 -1490.
AMA StyleSorin T. Schiop, Mohamad Al Hassan, Adriana F. Sestras, Monica Boscaiu, Radu E. Sestras, Oscar Vicente. Biochemical responses to drought, at the seedling stage, of several Romanian Carpathian populations of Norway spruce (Picea abies L. Karst). Trees. 2017; 31 (5):1479-1490.
Chicago/Turabian StyleSorin T. Schiop; Mohamad Al Hassan; Adriana F. Sestras; Monica Boscaiu; Radu E. Sestras; Oscar Vicente. 2017. "Biochemical responses to drought, at the seedling stage, of several Romanian Carpathian populations of Norway spruce (Picea abies L. Karst)." Trees 31, no. 5: 1479-1490.
Alina Vilcan (Truta); Lucica Mihalte (Crisan); Adriana F. Sestras; Liviu Holonec; Radu E. Sestras. Genetic variation and potential genetic resources of several Romanian larch populations. TURKISH JOURNAL OF AGRICULTURE AND FORESTRY 2017, 41, 82 -91.
AMA StyleAlina Vilcan (Truta), Lucica Mihalte (Crisan), Adriana F. Sestras, Liviu Holonec, Radu E. Sestras. Genetic variation and potential genetic resources of several Romanian larch populations. TURKISH JOURNAL OF AGRICULTURE AND FORESTRY. 2017; 41 ():82-91.
Chicago/Turabian StyleAlina Vilcan (Truta); Lucica Mihalte (Crisan); Adriana F. Sestras; Liviu Holonec; Radu E. Sestras. 2017. "Genetic variation and potential genetic resources of several Romanian larch populations." TURKISH JOURNAL OF AGRICULTURE AND FORESTRY 41, no. : 82-91.
Drought tolerance was evaluated in twelve cultivars of three ornamentalTagetesspecies (T. patula,T. tenuifoliaandT. erecta). A stress treatment was performed by completely stopping watering of plants maintained in controlled greenhouse conditions. After three weeks, several plant growth parameters (stem length (SL), fresh weight (FW) and water content (WC)), photosynthetic pigments (chlorophylls and carotenoids (Car)), osmolytes (proline (Pro), glycine betaine (GB) and total soluble sugars (TSS)), an oxidative stress maker (malondialdehyde (MDA)) and antioxidants (total phenolic compounds (TPC) and total flavonoids (TF)) were measured. Considerable differences in the evaluated traits were found among the control and drought-stressed plants. Drought stress generally caused a marked reduction in plant growth and carotenoid pigments, and an increase in soluble solutes and oxidative stress. For most cultivars, proline levels in stressed plants increased between 30 and 70-fold compared to the corresponding controls. According to the different measured parameters, on averageT. erectaproved to be more tolerant to drought thanT. patulaandT. tenuifolia. However, a considerable variation in the tolerance to drought was found within each species. The traits with greater association to drought tolerance as well as the most tolerant cultivars could be clearly identified in a principal components analysis (PCA). Overall, our results indicate that drought tolerant cultivars ofTagetescan be identified at early stages using a combination of plant growth and biochemical markers.
Raluca Cicevan; Mohamad Al Hassan; Adriana F. Sestras; Jaime Prohens; Oscar Vicente; Radu E. Sestras; Monica Boscaiu. Screening for drought tolerance in cultivars of the ornamental genusTagetes(Asteraceae). PeerJ 2016, 4, e2133 .
AMA StyleRaluca Cicevan, Mohamad Al Hassan, Adriana F. Sestras, Jaime Prohens, Oscar Vicente, Radu E. Sestras, Monica Boscaiu. Screening for drought tolerance in cultivars of the ornamental genusTagetes(Asteraceae). PeerJ. 2016; 4 ():e2133.
Chicago/Turabian StyleRaluca Cicevan; Mohamad Al Hassan; Adriana F. Sestras; Jaime Prohens; Oscar Vicente; Radu E. Sestras; Monica Boscaiu. 2016. "Screening for drought tolerance in cultivars of the ornamental genusTagetes(Asteraceae)." PeerJ 4, no. : e2133.
The Norway spruce (Picea abies), the most important tree species in European forests, is relatively sensitive to salt and does not grow in natural saline environments. Yet many trees are actually exposed to salt stress due to the common practice of de-icing of mountain roads in winter, using large amounts of NaCl. To help develop strategies for an appropriate use of reproductive seed material on reforestation sites, ensuring better chances of seedling survival in salt-affected areas, we have studied the responses of young spruce seedlings to salt treatments. The specific aim of the work was to identify the optimal salt stress biomarkers in Picea abies, using as experimental material seedlings obtained by germination of seeds with origin in seven populations from the Romanian Carpathian Mountains. These responses included general, conserved reactions such as the accumulation of ions and different osmolytes in the seedlings needles, reduction in photosynthetic pigments levels, or activation of antioxidant systems. Although changes in the contents of different compounds involved in these reactions can be associated to the degree of stress affecting the plants, we propose that the (decreasing) levels of total phenolics or total carotenoids and the (increasing) levels of Na+ or K+ ions in Picea abies needles, should be considered as the most reliable and useful biomarkers for salt stress in this species. They all show very high correlation with the intensity of salt stress, independently of the genetic background of the seeds parental population, and relatively easy, quantitative assays are available to determine their concentrations, requiring simple equipment and little amount of plant material.
Sorin T. Schiop; Mohamad Al Hassan; Adriana F. Sestras; Monica Boscaiu; Radu E. Sestras; Oscar Vicente. Identification of Salt Stress Biomarkers in Romanian Carpathian Populations of Picea abies (L.) Karst. PLOS ONE 2015, 10, e0135419 .
AMA StyleSorin T. Schiop, Mohamad Al Hassan, Adriana F. Sestras, Monica Boscaiu, Radu E. Sestras, Oscar Vicente. Identification of Salt Stress Biomarkers in Romanian Carpathian Populations of Picea abies (L.) Karst. PLOS ONE. 2015; 10 (8):e0135419.
Chicago/Turabian StyleSorin T. Schiop; Mohamad Al Hassan; Adriana F. Sestras; Monica Boscaiu; Radu E. Sestras; Oscar Vicente. 2015. "Identification of Salt Stress Biomarkers in Romanian Carpathian Populations of Picea abies (L.) Karst." PLOS ONE 10, no. 8: e0135419.
A total of 188 German and 28 Romanian pear accessions were evaluated using flow cytometry and molecular markers to detect homonymous and synonymous genotypes, triploids, which are not useful for breeding, and genotypes having favorable traits for breeding. Genotyping was done using a set of 11 simple sequence repeat markers proposed by the European Cooperative Program for Plant Genetic Resources, along with a standard set of six reference pear genotypes used in previous studies. The observed number of alleles ranged between 21 and 38 per locus. Twenty-three accessions could be confirmed as triploids. Because of the expected cross-incompatibility they are only of limited interest for breeding. The study confirmed that 180 of the 188 tested German accessions represent unique genotypes, while some accessions were found to be genetically identical. In parallel additional markers, which are known to be linked to resistance/susceptibility to major pathogens (e.g. black spot, aphid, fire blight, and apple scab), were applied to the German accessions. The comparative screening of the Romanian pear genetic resources demonstrated that only a few accessions with identical/very similar names to German accessions really have an identical fingerprint. Mislabeling and homonymous designation of different genotypes seems to occur frequently in different collections.
Melinda Puskás; Monika Höfer; Radu E. Sestras; Andreas Peil; Adriana F. Sestras; Magda-Viola Hanke; Henryk Flachowsky. Molecular and flow cytometric evaluation of pear (Pyrus L.) genetic resources of the German and Romanian national fruit collections. Genetic Resources and Crop Evolution 2015, 63, 1023 -1033.
AMA StyleMelinda Puskás, Monika Höfer, Radu E. Sestras, Andreas Peil, Adriana F. Sestras, Magda-Viola Hanke, Henryk Flachowsky. Molecular and flow cytometric evaluation of pear (Pyrus L.) genetic resources of the German and Romanian national fruit collections. Genetic Resources and Crop Evolution. 2015; 63 (6):1023-1033.
Chicago/Turabian StyleMelinda Puskás; Monika Höfer; Radu E. Sestras; Andreas Peil; Adriana F. Sestras; Magda-Viola Hanke; Henryk Flachowsky. 2015. "Molecular and flow cytometric evaluation of pear (Pyrus L.) genetic resources of the German and Romanian national fruit collections." Genetic Resources and Crop Evolution 63, no. 6: 1023-1033.
The potential of testing new apple cultivars and the possibility to induce valuable traits is directly dependent on the availability of sufficient genetic diversity, while apple breeding has narrowed the genetic ground of commercial cultivars. Wild species were studied in regard to their influence upon progenies and their capacity to enlarge apple genetic diversity. The interspecific seedlings were framed in five biparental mating (paired crosses), in which Malus species were crossed with different cultivars, obtaining half-sib families. The number of F1 progenies per combination varied from 31 (Cluj 218/2 × M. floribunda) up to 142 (Reinette Baumann × M. floribunda), with a total of 1650 hybrids F1. The influences upon vigour and juvenile period and possible correlation among fruit size and taste were analyzed. Juvenile period varied from 6.00 (M. zumi × Jonathan) to 9.31 years (Cluj 218/2 × M. floribunda). Data based on correlation coefficient illustrated that the fructification year was not influenced by the vigour of trees. The highest value of correlation for fruit’s size and taste was obtained among M. coronaria hybrids. This result might suggest that once the fruit are larger, there is a high chance the taste is also more appreciative and fruit quality for mouth feels increase. Depending on the parental formula, additive effects may be inferior compared to genetic effects of dominance and epistasis. Although M. zumi and M. floribunda achieved the same genetic gain (0.31), M. zumi had a higher expected selection response for fruit size. The difficulty of obtaining seedlings with tasty and large fruit when wild Malus species are used as genitors is resulting from the values of expected selection response data, but in the same time results confirm that wild Malus species are suitable resources for genetic variability, both for dessert and ornamental apple cultivars.
Catalina Dan; Adriana F. Sestras; Calin Bozdog; Radu E. Sestras. Investigation of wild species potential to increase genetic diversity useful for apple breeding. Genetika 2015, 47, 993 -1011.
AMA StyleCatalina Dan, Adriana F. Sestras, Calin Bozdog, Radu E. Sestras. Investigation of wild species potential to increase genetic diversity useful for apple breeding. Genetika. 2015; 47 (3):993-1011.
Chicago/Turabian StyleCatalina Dan; Adriana F. Sestras; Calin Bozdog; Radu E. Sestras. 2015. "Investigation of wild species potential to increase genetic diversity useful for apple breeding." Genetika 47, no. 3: 993-1011.
Jegatheswaran Ratnasingam; Cristina Vacalie; Adriana F. Sestras; Florin Ioras. Public Perception of Forestry Practices in Malaysia. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2014, 42, 1 .
AMA StyleJegatheswaran Ratnasingam, Cristina Vacalie, Adriana F. Sestras, Florin Ioras. Public Perception of Forestry Practices in Malaysia. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2014; 42 (1):1.
Chicago/Turabian StyleJegatheswaran Ratnasingam; Cristina Vacalie; Adriana F. Sestras; Florin Ioras. 2014. "Public Perception of Forestry Practices in Malaysia." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 42, no. 1: 1.
M. Militaru; N. Braniste; M. Butac; Adriana F. Sestras; T. Sotiropoulos; M. Lukic; B. Ambrozič Turk; V. Dzhuvinov. REVIEW OF POME FRUIT BREEDING IN BALKAN. Acta Horticulturae 2013, 177 -184.
AMA StyleM. Militaru, N. Braniste, M. Butac, Adriana F. Sestras, T. Sotiropoulos, M. Lukic, B. Ambrozič Turk, V. Dzhuvinov. REVIEW OF POME FRUIT BREEDING IN BALKAN. Acta Horticulturae. 2013; (981):177-184.
Chicago/Turabian StyleM. Militaru; N. Braniste; M. Butac; Adriana F. Sestras; T. Sotiropoulos; M. Lukic; B. Ambrozič Turk; V. Dzhuvinov. 2013. "REVIEW OF POME FRUIT BREEDING IN BALKAN." Acta Horticulturae , no. 981: 177-184.