Cotton (Gossypium spp.), a crucial cash crop in the United States, requires the constant monitoring of growth parameters for informed decision-making. Recently, forecasting models have gained prominence for predicting canopy indicators, aiding in-season planning and management decisions to optimize cotton production. This study employed unmanned aerial system (UAS) technology to collect canopy cover (CC) data from a 40-hectare cotton field in Driscoll, Texas, in 2020 and 2021. Long short-term memory (LSTM) models, trained using 2020 data, were subsequently applied to forecast the CC values for 2021. These models were compared with real-time auto-regressive integrated moving average (ARIMA) models to assess their effectiveness in predicting the CC values up to 14 days in advance, starting from the 28th day after crop emergence. The results showed that multiple-input multi-step output LSTM models achieved higher accuracy in predicting the in-season CC values during the early growth stages (up to the 56th day), with an average testing RMSE of 3.86, significantly lower than other single-input LSTM models. Conversely, when sufficient testing data are available, single-input stacked-LSTM models demonstrated precision in CC predictions for later stages, achieving an average RMSE of 3.06. These findings highlight the potential of LSTM models for in-season CC forecasting, facilitating effective management strategies in cotton production.

Plant growth analysis provides insight regarding the variation behind yield differences in tested genotypes for plant breeders, but adopting this application solely for traditional plant phenotyping remains challenging. Here, we propose a procedure of using uncrewed aerial systems (UAS) to obtain successive phenotype data for growth analysis. The objectives of this study were to obtain high-temporal UAS-based phenotype data for growth analysis and investigate the correlation between the UAS-based phenotype and biomass yield. Seven different energy cane genotypes were grown in a random complete block design with four replications. Twenty-six UAS flight missions were flown throughout the growing season, and canopy cover (CC) and canopy height (CH) measurements were extracted. A five-parameter logistic (5PL) function was fitted through these temporal measurements of CC and CH. The first- and second-order derivatives of this function were calculated to obtain several growth parameters, which were then used to assess the growth of different genotypes with respect to weed competitiveness and biomass yield traits. The results show that CC and CH growth rates significantly differed among genotypes. TH16-16 was outstanding for its ground cover growth; therefore, it was identified as a weed-competitive genotype. Furthermore, TH16-22 had a higher CH maximum growth rate per day, yielding a higher biomass compared to other genotypes. The CH-based multi-temporal data as well as the growth parameters had a better relationship with biomass yield. This study highlights the application of UAS-based high-throughput phenotyping (HTP), along with growth analysis, for assisting plant breeders in decision-making.