The effects of high-intensity light on the pigment content, photosynthetic rate, and fluorescence parameters of photosystem II in high-pigment tomato mutants (hp 3005) and low-pigment mutants (lp 3617) were investigated. This study also evaluated the dry weight percentage of low molecular weight antioxidant capacity, expression patterns of some photoreceptor-regulated genes, and structural aspects of leaf mesophyll cells. The 3005 mutant displayed increased levels of photosynthetic pigments and anthocyanins, whereas the 3617 mutant demonstrated a heightened content of ultraviolet-absorbing pigments. The photosynthetic rate, photosystem II activity, antioxidant capacity, and carotenoid content were most pronounced in the high-pigment mutant after 72 h exposure to intense light. This mutant also exhibited an increase in leaf thickness and water content when exposed to high-intensity light, suggesting superior physiological adaptability and reduced photoinhibition. Our findings indicate that the enhanced adaptability of the high-pigment mutant might be attributed to increased flavonoid and carotenoid contents, leading to augmented expression of key genes associated with pigment synthesis and light regulation.

Environmental factors, such as light of different spectral compositions and temperature, can change the level of activated photoreceptors which, in turn, can affect the biosynthesis of secondary metabolites in the cells of green fruit. By briefly irradiating the harvested fruit of Capsicum annuum L. hot peppers with red light (RL, maximum 660 nm) and far-red light (FRL, maximum 730 nm) and by keeping them at a low temperature, we attempted to determine whether the state of phytochromes in fruit affects the biosynthesis of secondary metabolites. Using HPLC, we analysed the qualitative composition and quantitative content of the main carotenoids and alkaloids and the chlorophylls and ascorbate, in pepper fruit exposed to the above factors. We measured the parameters characterising the primary photochemical processes of photosynthesis and the transcript levels of genes encoding capsaicin biosynthesis enzymes. The total carotenoids content in the fruit increased most noticeably after 24 h of RL irradiation (more than 3.5 times compared to the initial value), and the most significant change in the composition of carotenoids occurred when the fruit was irradiated with FRL for 72 h. The capsaicin alkaloid content increased markedly after 72 h of FRL irradiation (more than 8 times compared to the initial value). It was suggested that decrease in the activity of phytochromes due to a low temperature or FRL may result in an increase in the expression of the PAL and CAM genes.

The aim of this study was to investigate the effect of light quality (white fluorescent light, WFL, containing UV components), red light (RL, 660 nm), blue light (BL, 450 nm), and white LED light (WL, 450 + 580 nm) on the components of the cellular antioxidant system in Pinus sylvestris L. in needles, roots, and hypocotyls, focusing on the accumulation of key secondary metabolites and the expression of related genes. The qualitative and quantitative composition of carotenoids; the content of the main photosynthetic pigments, phenolic compounds, flavonoids (catechins, proanthocyanidins, anthocyanins), ascorbate, and glutathione; the activity of the main antioxidant enzymes; the content of hydrogen peroxide; and the intensity of lipid peroxidation (MDA and 4-HNE contents) were determined. RL resulted in an increase in the content of hydrogen peroxide and 4-HNE, as well as the total fraction of flavonoids in the needles. It also enhanced the expression of several PR (pathogen-related) genes compared to BL and WL. WFL increased the content of phenols, including flavonoids, and enhanced the overall activity of low-molecular antioxidants in needles and hypocotyls. BL increased the content of ascorbate and glutathione, including reduced glutathione, in the needles and simultaneously decreased the activity of peroxidases. Thus, by modifying the light quality, it is possible to regulate the accumulation of secondary metabolites in pine roots and needles, thereby influencing their resistance to various biotic and abiotic stressors.

The effects of high-intensity blue light (HIBL, 500/1000 µmol m⁻²s⁻¹, 450 nm) on Solanum lycopersicum mutants with high pigment (hp) and low pigment (lp) levels and cryptochrome 1 (cry1) deficiency on photosynthesis, chlorophylls, phenols, anthocyanins, nonenzymatic antioxidant activity, carotenoid composition, and the expression of light-dependent genes were investigated. The plants, grown under white light for 42 days, were exposed to HIBL for 72 h. The hp mutant quickly adapted to 500 µmol m⁻²s⁻¹ HIBL, exhibiting enhanced photosynthesis, increased anthocyanin and carotenoids (beta-carotene, zeaxanthin), and increased expression of key genes involved in pigment biosynthesis (PSY1, PAL1, CHS, ANS) and PSII proteins along with an increase in nonenzymatic antioxidant activity. At 1000 µmol m⁻²s⁻¹ HIBL, the lp mutant showed the highest photosynthetic activity, enhanced expression of genes associated with PSII external proteins (psbO, psbP, psbQ), and increased in neoxanthin content. This mutant demonstrated greater resistance at the higher HIBL, demonstrating increased stomatal conductance and photosynthesis rate. The cry1 mutant exhibited the highest non-photochemical quenching (NPQ) but had the lowest pigment contents and decreased photosynthetic rate and PSII activity, highlighting the critical role of CRY1 in adaptation to HIBL. The hp and lp mutants use distinct adaptation strategies, which are significantly hindered by the cry1 mutation. The pigment content appears to be crucial for adaptation at moderate HIBL doses, while CRY1 content and stomatal activity become more critical at higher doses.

— The effect of singlet oxygen was studied in the system of Allochromatium ( Alc.) vinosumMSU membranes, rose bengal, and light (547−600nm) with and without a quencher. In the system without...