Taking sustainable agriculture measures is critical to effectively cope with the effect of the increasing population on water shortage. Straw mulching and reduced tillage are the most successful measures adopted in arid and semi-arid regions which affect crop production by changing the crop environment. This review focuses on the effects of tillage and mulching on the soil environment, including soil organic matter, soil moisture, soil temperature, soil microorganisms, soil enzyme activity, soil fertility, soil carbon emissions, pests, weeds, and soil erosion. In addition, water use efficiency and crop production are discussed under different tillage measures. Straw mulching can increase soil organic matter content, adjust soil moisture, and prevent water loss and drought; however, it can also lead to an increase in pests and diseases, and change the structure of the soil microbial community. Straw mulching can significantly enhance WUE (water use effectively) and yield. Reducing tillage maintains soil integrity, which is conducive to soil and water conservation, but could negatively impact crop yield and WUE. Precise field management measures, taken according to crop varieties and local conditions, not only ensure the high yield of crops but also protect the environment.

This study aimed to examine the effect of nitrogen (N) application rate and time on yield, grain filling, starch metabolizing enzymes, and hormones of maize based on a long-term field experiment initiated in 2012. The total N fertilizer dose [(0 (N0), 100 (N1), 200 (N2), and 300 (N3) kg N ha^–1] was split into two (T1, one-third at sowing and two-thirds at the six-leaf stage) or three (T2, one-third each at sowing, six-leaf, and eleven-leaf stage) times application. The results showed that the highest yield was obtained under N3T2, N2T1, and N3T2 in 2018, 2019, and 2020, which was 222.49, 185.31, and 194.00% than that of N0 in each year, respectively. N2 and N3 significantly increased the yield through enhancing ears ha^–1, grains per plant, and 100-grain weight; however, N2 and N3 did not show a significant difference in yield and above-yield components. In addition, N application time did not significantly change yield under the same N rate. N0 limited the activities of starch metabolizing enzymes, resulting in insufficient accumulation of sucrose and starch. The contents of indole-3-acetic acid, cytokinin, abscisic acid, and gibberellin were decreased under N0 during grain filling. The average grain-filling rate and maximum grain-filling rate (G_max) and grain weight increment achieving G_max increased under N2 and N3, and the grain-filling parameters were positively correlated with 100-grain weight. In conclusion, 200 kg N ha^–1 with one-third application at sowing and two-thirds application at the six-leaf stage is a suitable N supply way to improve starch metabolizing enzymes, regulate hormone content, and enhance grain-filling rates, and thus increasing the maize yield in the semiarid Loess Plateau of China.

Soil physiochemical properties are regulated by cropping practices, but little is known about how tillage influences soil microbial community diversity and functions. Here, we assessed soil bacterial community assembly and functional profiles in relation to tillage. Soils, collected in 2018 from a 17-year field experiment in northwestern China, were analyzed using high-throughput sequencing and the PICRUSt approach. The taxonomic diversity of bacterial communities was dominated primarily by the phyla Proteobacteria (32–56%), Bacteroidetes (12–33%), and Actinobacteria (17–27%). Alpha diversity (Chao1, Shannon, Simpson, and operational taxonomic unit (OTU) richness) was highest under no-tillage with crop residue removed (NT). Crop residue retention on the soil surface (NTS) or incorporated into soil (TS) promoted the abundance of Proteobacteria by 16 to 74% as compared to conventional tillage (T). Tillage practices mainly affected the pathways of soil metabolism, genetic information processing, and environmental information processing. Soil organic C and NH₄–N were the principal contributors to the diversity and composition of soil microbiota, whereas soil pH, total nitrogen, total P, and moisture had little effect. Our results suggest that long-term conservation practices with no-tillage and crop residue retention shape soil bacterial community composition through modifying soil physicochemical properties and promoting the metabolic function of soil microbiomes.

Nitrous oxide (N₂O) emissions are a major source of gaseous nitrogen loss, causing environmental pollution. The low organic content in the Loess Plateau region, coupled with the high fertilizer demand of maize, further exacerbates these N losses. N fertilizers play a primary role in N₂O emissions by influencing soil denitrifying bacteria, however, the underlying microbial mechanisms that contribute to N₂O emissions have not been fully explored. Therefore, the research aimed to gain insights into the intricate relationships between N fertilization, soil denitrification, N₂O emissions, potential denitrification activity (PDA), and maize nitrogen use efficiency (NUE) in semi-arid regions. Four nitrogen (N) fertilizer rates, namely N0, N1, N2, and N3 (representing 0, 100, 200, and 300 kg ha⁻¹ yr.⁻¹, respectively) were applied to maize field. The cumulative N₂O emissions were 32 and 33% higher under N2 and 37 and 39% higher under N3 in the 2020 and 2021, respectively, than the N0 treatment. N fertilization rates impacted the abundance, composition, and network of soil denitrifying communities (nirS and nosZ) in the bulk and rhizosphere soil. Additionally, within the nirS community, the genera Cupriavidus and Rhodanobacter were associated with N₂O emissions. Conversely, in the nosZ denitrifier, the genera Azospirillum, Mesorhizobium, and Microvirga in the bulk and rhizosphere soil reduced N₂O emissions. Further analysis using both random forest and structural equation model (SEM) revealed that specific soil properties (pH, NO₃⁻-N, SOC, SWC, and DON), and the presence of nirS-harboring denitrification, were positively associated with PDA activities, respectively, and exhibited a significant association to N₂O emissions and PDA activities but expressed a negative effect on maize NUE. However, nosZ-harboring denitrification showed an opposite trend, suggesting different effects on these variables. Our findings suggest that N fertilization promoted microbial growth and N₂O emissions by increasing the abundance of nirS and nosZ denitrifiers and altering the composition of their communities. This study provides new insights into the relationships among soil microbiome, maize productivity, NUE, and soil N₂O emissions in semi-arid regions.